Long Acellular Nerve Transplants for Allogeneic Grafting and the Effects of Basic Fibroblast Growth Factor on the Growth of Regenerating Axons in Dogs: A Preliminary Report

Idé, Chizuka; Tohyama, Koujiro; Tajima, Kazuki; Endoh, Katsuaki; Sano, Kei-ichi; Tamura, Makoto; Mizoguchi, Akira; Kitada, Masaaki; Morihara, Toru; Shirasu, M

doi:10.1006/exnr.1998.6921

Cited by 75 publications

(57 citation statements)

References 40 publications

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“…4 D, E: arrows) in sections from grafts that had been implanted. As has been reported by others (Ide et al, 1998;Krekoski et al, 2001), such seemingly enlarged endoneurial tubes also were found in control (cellular) grafts (data not shown).…”

Section: Effects Of Electrical Stimulation On Axon Regeneration Throusupporting

confidence: 87%

“…In these grafts, Schwann cells have been destroyed, leaving behind endoneurial tubes and their associated extracellular matrix (Ide et al, 1998;Krekoski et al, 2001) ( Fig. 4C-E).…”

Section: Discussionmentioning

confidence: 99%

“…In the third group, segments of CF nerves were used from wild type C57BL/6J mice which were rendered acellular (Ide et al, 1998;Krekoski et al, 2001). Segments of nerves were removed from the euthanized wild type donor mouse and frozen in liquid nitrogen for two minutes.…”

Section: Introductionmentioning

confidence: 99%

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Electrical stimulation promotes peripheral axon regeneration by enhanced neuronal neurotrophin signaling

et al. 2006

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Electrical stimulation of cut peripheral nerves at the time of their surgical repair results in an enhancement of axon regeneration. Regeneration of axons through nerve allografts was used to evaluate whether this effect is due to an augmentation of cell autonomous neurotrophin signaling in the axons or signaling from neurotrophins produced in the surrounding environment. In the thy-1-YFP-H mouse, a single one hour application of electrical stimulation at the time of surgical repair of the cut common fibular nerve results in a significant increase in the proportion of YFP+ dorsal root ganglion neurons that were also immunoreactive for BDNF or trkB as well as an increase in the length of regenerating axons through allografts from wild type litter mates, both one and two weeks later. Axon growth through allografts from neurotrophin-4/5 knockout mice or grafts made acellular by repeated cycles of freezing and thawing is normally very poor, but electrical stimulation results in a growth of axons through these grafts which is similar to that observed through grafts from wild type mice after electrical stimulation. When cut nerves in NT-4/5 knockout mice were electrically stimulated, no enhancement of axon regeneration was found. Electrical stimulation thus produces a potent enhancement of the regeneration of axons in cut peripheral nerves which is independent of neurotrophin production by cells in their surrounding environment but is dependent on stimulation of trkB and its ligands in the regenerating axons themselves.

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Section: Effects Of Electrical Stimulation On Axon Regeneration Throusupporting

confidence: 87%

“…In these grafts, Schwann cells have been destroyed, leaving behind endoneurial tubes and their associated extracellular matrix (Ide et al, 1998;Krekoski et al, 2001) ( Fig. 4C-E).…”

Section: Discussionmentioning

confidence: 99%

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Electrical stimulation promotes peripheral axon regeneration by enhanced neuronal neurotrophin signaling

et al. 2006

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“…To overcome this difficulty, we attempt to perform frozen allogeneic nerve grafts [5] in dogs. No immunosuppressive reagents are required with this method, and successful axonal regeneration and myelination has been confirmed by electron microscopy in mice [13], rats [14], rabbits [20], dogs [6] and monkeys [19]. Given this morphological evidence, reasonable functional recovery appears likely.…”

mentioning

confidence: 81%

Morphological Variations in Brachial Plexus of Beagle Dogs: Evaluation of Utility as Sources of Allogeneic Nerve Grafts

Nakamura

Tomizawa

Tohyama

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. Basic studies were carried out to apply frozen allogeneic nerve grafts in dogs after wide-ranging defects of the brachial plexus due to surgical resection of tumor. In this study, morphological variations in branching patterns of the brachial plexus were examined in ten beagle dogs, to evaluate whether the brachial plexus might represent a useful source of allogeneic nerve grafts. Spatial relationships between the axillary lymph node, which had the possibility of carcinomatous metastasis, and the musculocutaneous (MC) nerve, which was important for the function of the forelimbs, were also investigated. In all ten cases examined, the brachial plexus received ventral roots from the fifth cervical nerve to the first thoracic nerve. No significant variation in the branching pattern was found in any nerve except the phrenic, MC and dorsal thoracic nerves. Four communicating branches were observed and had some morphological variations which might be negligible for nerve grafting. Considering previous physiological and anatomical reports, the most important nerve to be reunited in graft operations for functional recovery is the radial nerve. The MC nerve and median or ulnar nerve sh ould also be considered as possibilities for reuniting. Distances between the axillary lymph nodes and the MC nerve ranged from 11.2 mm to 21 mm (mean ± SD: 16.1 ± 2.3 mm). In conclusion, it was suggested that morphological variations in the brachial plexus were technically acceptable to apply allogeneic nerve grafts at least in beagle dogs. KEY WORDS: axillary lymph node, beagle dog, brachial plexus, branching pattern, variation.

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“…When bFGF has been applied distal to crush lesions of rat sciatic nerves, nerve regeneration is improved (Vergara et al, 1993). In dogs, bFGF has been shown to improve nerve regeneration in the presence of acellular SC basal laminae grafts (Ide et al, 1998). Early experiments demonstrating the ineffectiveness of bFGF to enhance neuronal survival in vivo may have been handicapped from an inadequate method of bFGF delivery (Li et aI., 1994).…”

Section: Igf-i In Neuronal Survival and Target Reinnervationmentioning

confidence: 99%

Alterations in the Local Axonal Environment Influence Target Reinnervation and Neuronal Survival After PostnataI Axotomy

Dainer¹

2000

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Long Acellular Nerve Transplants for Allogeneic Grafting and the Effects of Basic Fibroblast Growth Factor on the Growth of Regenerating Axons in Dogs: A Preliminary Report

Cited by 75 publications

References 40 publications

Electrical stimulation promotes peripheral axon regeneration by enhanced neuronal neurotrophin signaling

Electrical stimulation promotes peripheral axon regeneration by enhanced neuronal neurotrophin signaling

Morphological Variations in Brachial Plexus of Beagle Dogs: Evaluation of Utility as Sources of Allogeneic Nerve Grafts

Alterations in the Local Axonal Environment Influence Target Reinnervation and Neuronal Survival After PostnataI Axotomy

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