Lomofungin, a New Antibiotic Produced by Streptomyces lomondensis sp. n

Johnson, L. E.; Dietz, Alma

doi:10.1128/aem.17.5.755-759.1969

Cited by 27 publications

(19 citation statements)

References 5 publications

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“…Lomofungin (5-formyl-1-methoxycarbonyl-4,6,8trihydroxyphenazine) is an antibiotic from Streptomyces lomodensis (Johnson & Dietz, 1969). It rapidly inhibits RNA synthesis in yeast, but has no immediate effect on cell growth or protein synthesis (Gottlieb & Nicolas, 1969;Lampen et al, 1973;Fraser et al, 1973;Cannon et al, 1973).…”

mentioning

confidence: 99%

The mechanism of inhibition of ribonucleic acid synthesis by 8-hydroxyquinoline and the antibiotic lomofungin.

Fraser

Creanor

1975

Biochemical Journal

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RNA synthesis in yeast is rapidly inhibited by 8-hydroxyquinoline and the phenazine antibiotic lomofungin (5-formyl-1-methoxycarbonyl-4,6,8-trihydroxyphenazine). It is shown that lomofungin, like 8-hydroxyquinoline, is a chelating agent for bivalent cations. The mechanism of inhibition of RNA synthesis by lomofungin and 8-hydroxyquinoline was investigated in experiments with isolated Escherichia coli RNA polymerase. The results show that both inhibitors are capable of inhibiting polymerase activity solely by chelating the dissociable cations Mn2+ and Mg2+. Evidence is presented which shows that inhibition may occur in the absence of any direct contact between the RNA polymerase or DNA template and the inhibitor. The possibility that inhibition might also occur by chelation of the Zn2+, which is tightly bound to the polymerase, is discussed: it is concluded that lomofungin or 8-hydroxyquinoline is likely to inhibit the enzyme by removal of Mn2+ and Mg2+ before chelating the Zn2+. On the basis of inhibition by chelation of Mn2+ and Mg2+, explanations are proposed for why lomofungin and 8-hydroxyquinoline inhibit synthesis of ribosomal and polydisperse RNA more than that of 5S RNA and tRNA, and for why protein synthesis is not immediately inhibited in the intact yeast cell.

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The mechanism of inhibition of ribonucleic acid synthesis by 8-hydroxyquinoline and the antibiotic lomofungin.

Fraser

Creanor

1975

Biochemical Journal

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“…In addition to proflavine, inhibitors of RNA synthesis which proved effective in preventing isomerase production included acriflavine neutral, Lomofungin (Upjohn) (1,11), and 8-quinolinol (6). Proflavine was chosen for use in further experiments because its mechanism of action (intercalation) is well established (14) and it is readily available.…”

Section: Resultsmentioning

confidence: 99%

Regulation of isopropylmalate isomerase synthesis in Neurospora crassa

Reichenbecher¹,

Fischer²,

Gross³

1978

J Bacteriol

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The capacity to synthetize isopropylmalate isomerase (EC 4.2.1.33) by Neurospora crassa increased during induction in the presence of cycloheximide but was inhibited by proflavine and other inhibitors of RNA synthesis. Turnover of the enzyme once formed appeared negligible, but the message (measured as enzyme-forming capacity) had a half-life of 4 to 8 min. A comparison of the kinetics of induction in the wild type and a newly isolated alpha-isopropylmalate-permeable strain suggested strongly that feedback control by leucine of alpha-isopropylmalate production can adequately serve as the primary physiological regulator of endogenous inducer concentration. Genetic data are presented which implicate the involvement of two unlinked genes, ipm-1 and ipm-2, in determining permeation of alpha-isopropylmalate.

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“…(13). These include S. griseoluteus (14), S. kebangsaanensis (13), S. lomondensis (15), S. anulatus (16), S. niveus (17) and several other species (14,18,19). Luo et al (14) also noted that members of Streptomyces have commonly been used as a source of various phenazines complex such as griseolutein, phenacetin, lomofungin, and 5,10-dihydrophenazine.…”

Section: Introductionmentioning

confidence: 99%

Isolation and structure elucidation of phenazine derivative from Streptomyces sp. strain UICC B-92 isolated from Neesia altissima (Malvaceae)

Pratiwi¹,

Hidayat²,

Hanafi³

et al. 2020

IJM

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Background and Objectives: Endophytic actinomycetes have been known as a promising source for new antibiotics discov- ery against susceptible and resistant forms of pathogenic microorganisms. This study was aimed at determining antibacterial compound from Streptomyces sp. strain B-92 isolated from a medicinal plant Neesia altissima. Materials and Methods: Streptomyces sp. strain UICC B-92 was endophytic actinomycetes of N. altissima that obtained from Universitas Indonesia Culture Collection (UICC). Isolation and determination of bioactive compound were carried out using thin layer chromatography (TLC), nuclear magnetic resonance spectroscopy (NMR), and liquid chromatography mass spectrometry (LC-MS) analyses. An in vitro antibacterial assay of pure bioactive compound from the endophytic actinomycetes strain was performed against Bacillus cereus strain ATCC 10876, Escherichia coli strain ATCC 25922, Salmonella typhimurium strain ATCC 25241, Shigella flexneri strain ATCC 12022 and Staphylococcus aureus strain ATCC 25923. Results: The bioactive compound was identified as 4-((3S,4R,5S)-3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H- pyran-2-yloxy) phenazine-1-carboxylic acid. In vitro antimicrobial assay showed that bioactive compound of Streptomyces sp. strain UICC B-92 exhibited antagonistic activities against two Gram-positive bacteria, viz, B. cereus strain ATCC 10876 and S. aureus strain ATCC 25923. Conclusion: The findings of this research showed that, bioactive compound of Streptomyces sp. strain UICC B-92 is sug- gested a new compound based on glycoside structure and its position.

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Lomofungin, a New Antibiotic Produced by Streptomyces lomondensis sp. n

Cited by 27 publications

References 5 publications

The mechanism of inhibition of ribonucleic acid synthesis by 8-hydroxyquinoline and the antibiotic lomofungin.

The mechanism of inhibition of ribonucleic acid synthesis by 8-hydroxyquinoline and the antibiotic lomofungin.

Regulation of isopropylmalate isomerase synthesis in Neurospora crassa

Isolation and structure elucidation of phenazine derivative from Streptomyces sp. strain UICC B-92 isolated from Neesia altissima (Malvaceae)

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