Loliolide Presents Antiapoptosis and Antiscratching Effects in Human Keratinocytes

Abstract

Loliolide is a monoterpenoid hydroxylactone present in freshwater algae that has anti-inflammatory and antiaging activity; however, its effects on ultraviolet-damaged skin have yet to be elucidated. This study investigated the antiapoptosis and wound-healing effects of loliolide using HaCaT cells (a human keratinocyte cell line). Loliolide inhibited the expression of reactive oxygen species (ROS) induced by ultraviolet radiation as well as wrinkle formation-related matrix metalloproteinase genes and increased the expression of the damage repair-related gene SIRT1. The apoptosis signaling pathway was confirmed by Western blot analysis, which showed that loliolide was able to reduce the expression of caspases 3, 8, and 9, which are related to ROS-induced apoptosis. In addition, Western blotting, reverse-transcription polymerase chain reaction (PCR), and real-time PCR analyses showed that loliolide enhanced the expression of the epidermal growth factor receptor signaling pathway (PI3K, AKT) and migration factors, such as K6, K16, and K17; keratinocyte growth factor; and inflammatory cytokines, such as interleukin (IL)-1, IL-17, and IL-22 expressed during the cellular scratching process, suggesting a putative wound-healing ability. Because of the antiapoptosis and antiscratching effects on skin of both loliolide and loliolide-rich Prasiola japonica ethanol extract, we consider the former to be an important compound used in the cosmeceutical industry.

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

“…We further evaluated the antiwrinkle effects of AL using keratinocytes and human dermal fibroblasts. Interestingly, AL increased the mRNA expression of SIRT-1 (Figure 2c), which has a notable function in cell survival and inflammatory processes [37][38][39]. Based on our results, it is assumed that AL protects cells through antioxidative effects, inhibits MMPs by regulating AP-1 signaling, and suppresses wrinkles by inhibiting elastase activity in keratinocytes.…”

“…AL suppressed the expression of MMP-1, -2, -3, and -9 in UVB-induced HaCaT cells in a dose-dependent manner. Additionally, the expression of SIRT-1, which has notable functions in cell survival and inflammatory processes [37][38][39], was increased to approximately its baseline level by AL ( Figure 2c). ROS, which are secondary messengers to activate mitogen-activated protein kinase (MPAK), promote the expression of MMPs due to induction of the AP-1 signaling pathway [36].…”

Comprehensive Metabolome Analysis of Fermented Aqueous Extracts of Viscum album L. by Liquid Chromatography−High Resolution Tandem Mass Spectrometry

“…Twenty terpenoids and their derivatives were annotated, and three of them (carveol, loliolide, and vomifoliol) have already been described in V. album [ 34 , 35 ]. The anticancerogenic activities of terpenoids, such as loliolide, curcumol, dehydrovomifoliol and blumenol A have already been tested in tumor cell lines such as colon cancer, KB human oral epidermoid carcinoma cells, and HT29 colorectal carcinoma cells [ 36 , 37 , 38 , 39 ].…”

Phytotoxic Activity and Growth Inhibitory Substances from Albizia richardiana (Voigt.) King & Prain

“…Loliolide has various pharmaceutical functions [62]. It has been applied for its antioxidant [68], anticancer [58], antiviral, anti-melanogenic, antiinflammatory, anti-aging [69], antituberculosis [70], antidiabetic [71], antibacterial, antifungal [64], and cell senescence inhibition activities [58]. Previously, the compounds dehydrovomifoliol and loliolide have also been identified from the plants Rollinia emarginata [72], Paspalum commersonii [73], and Vitex leptobotrys [74].…”