Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum

Abstract

Parkinsons Disease (PD) is the second most common neurodegenerative disease worldwide, and is characterized by a progressive degeneration of dopaminergic neurons. Without an effective treatment, it is crucial to find new therapeutic options to fight the neurodegenerative process, which may arise from marine resources. Accordingly, the goal of the present work was to evaluate the ability of the monoterpenoid lactone Loliolide, isolated from the green seaweed Codium tomentosum, to prevent neurological cell death mediated by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y cells and their anti-inflammatory effects in RAW 264.7 macrophages. Loliolide was obtained from the diethyl ether extract, purified through column chromatography and identified by NMR spectroscopy. The neuroprotective effects were evaluated by the MTT method. Cells´ exposure to 6-OHDA in the presence of Loliolide led to an increase of cells´ viability in 40%, and this effect was mediated by mitochondrial protection, reduction of oxidative stress condition and apoptosis, and inhibition of the NF-kB pathway. Additionally, Loliolide also suppressed nitric oxide production and inhibited the production of TNF-α and IL-6 pro-inflammatory cytokines. The results suggest that Loliolide can inspire the development of new neuroprotective therapeutic agents and thus, more detailed studies should be considered to validate its pharmacological potential.

Terpene-rich fractions of Ficus mucoso (Welw) modulate lipopolysaccharide-induced inflammatory mediators and aberrant permeability of the inner mitochondrial membrane in murine animal model

“…The monoterpene lactone, loliolide which was identi ed in both TRDF and TREF was recently described as a potent anti-in ammatory and neuroprotective metabolite (Silva et al, 2021). Similarly, aesculetin and several other coumarins derivatives are well known anti-in ammatory agents (Grover & Jachak, 2015).…”

Neuroprotective Effect of Luteolin-7-O-Glucoside against 6-OHDA-Induced Damage in Undifferentiated and RA-Differentiated SH-SY5Y Cells

“…The antioxidant activity of Lut7 was evaluated by the means of different methodologies, namely (a) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability [ 19 ]; (b) oxygen radical absorbance capacity (ORAC) [ 20 ]; and (c) ferric reducing antioxidant power (FRAP) assays [ 21 ] with slight adaptations [ 15 ]. Butylated hydroxytoluene (BHT) was used as a positive control for antioxidant activity.…”

“…Its potential benefits to the CNS include the decrease of microglia activation and neuronal protection [ 3 , 4 , 5 ]. However, the glycosylated form of luteolin, known as cyranoside or luteolin-7-O-glucoside (Lut7) (PubChem ID 5280637), was reported as a selective JNK3 inhibitor, five times more selective than luteolin [ 6 ], which plays a key role in neurodegenerative diseases [ 7 , 8 , 9 , 10 , 11 ], suggesting that patients with neurodegenerative diseases might benefit from a natural or bio-inspired product-based therapy [ 12 , 13 , 14 , 15 , 16 ].…”

Supercritical CO2 Extract from Microalga Tetradesmus obliquus: The Effect of High-Pressure Pre-Treatment

“…Monoterpenoid lactone loliolide was identified in both extracts. Loliolide was previously isolated from different seaweeds, such as Codium tomentosum [43], Sargassum horneri [44], and Undaria pinnatifida [45]. It is considered to possess antioxidant and anticancer activity.…”

“…It is considered to possess antioxidant and anticancer activity. In addition, it is suggested that loliolide could be applied in the development of new neuroprotective therapeutics for Parkinson's disease, due to its antioxidant, anti-inflammatory, and neuroprotective mechanisms [43]. Moreover, among representatives of terpenoids, isoamijiol and isoamijiol oxidation products were identified.…”