Loktanella sediminilitoris sp. nov., isolated from tidal flat sediment

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated D1-W3 T , was isolated from tidal flat sediment of the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain D1-W3 T grew optimally at pH 7.0-8.0, at 25 6C and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic analyses based on 16S rRNA gene sequences revealed that strain D1-W3 T fell within the clade comprising species of the genus Loktanella, clustering with the type strains of Loktanella tamlensis, Loktanella rosea and Loktanella maricola, with which it exhibited the highest 16S rRNA gene sequence similarity values (98.1-98.2 %). The 16S rRNA gene sequence similarity values between strain D1-W3 T and the type strains of other species of the genus Loktanella were in the range 93.5-96.5 %. The DNA G+C content of strain D1-W3 T was 58.1 mol% and the mean DNA-DNA hybridization values with L. tamlensis KCTC 12722 T , L. rosea LMG 22534 T and L. maricola DSW-18 T were 13-25 %. Strain D1-W3 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, showed that strain D1-W3 T could be differentiated from other species of the genus Loktanella. On the basis of the data presented, strain D1-W3 T represents a novel species of the genus Loktanella, for which the name Loktanella sediminilitoris sp. nov. is proposed. The type strain is D1-W3 T (5KCTC 32383 T 5CECT 8284 T ).The genus Loktanella, a member of the class Alphaproteobacteria, was created by Van Trappen et al. (2004) (Van Trappen et al., 2004;Lau et al., 2004; Ivanova et al., 2005; Weon et al., 2006;Yoon et al., 2007;Hosoya & Yokota, 2007;Moon et al., 2010;Lee, 2012). In this study, we describe a Loktanella-like bacterial strain, designated D1-W3 T , which was isolated from a tidal flat in the South Sea, South Korea. The aim of the present work was to determine the exact taxonomic position of strain D1-W3 T by using a polyphasic characterization that included the determination of chemotaxonomic and other phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and DNA-DNA hybridization studies.Strain D1-W3 T was isolated by the standard dilution plating technique at 25 u C on marine agar 2216 (MA; BD) and cultivated routinely under the same conditions. Strain D1-W3 T was maintained on MA at 4 u C for short-term preservation and as a glycerol suspension (20 %, w/v in distilled water) at 280 u C for long-term preservation. L. maricola DSW-18 T , L. rosea LMG 22534 T and L. tamlensis KCTC 12722 T were used as reference strains for DNA-DNA hybridization and phenotypic characterization. Cell morphology was examined by using light microscopy (BX51; Olympus) and transmission electron microscopy (JEM1010; JEOL). The latter technique was also used to ...

Loktanella maritima sp. nov. isolated from shallow marine sediments

“…The polar profile of L. sediminilitoris KCTC 32383 T differed from those of the other four strains studied in that phosphatidylethanolamine (PE) was detected. This finding is congruent with the original description by Park et al (2013a) who reported the presence of PC, PG, PE and an unknown aminolipid as dominant and DPG, an unknown phospholipid and an unknown lipid as minor components in L. sediminilitoris KCTC 32383 T . In addition, unknown phospholipids PL1-PL3 were found in the present study.…”

“…DResults differ from those reported by Park et al (2013a). dData not consistent with those reported by Yoon et al (2007).…”

“…agnita (Ivanova et al, 2005), L. sediminilitoris KCTC 32383 T (Park et al, 2013a) and L. cinnabarina (Tsubouchi et al, 2013), and bacteria not containing PE in their polar lipid profiles, L. pyoseonensis (Moon et al, 2010), L. hongkongensis (Tsubouchi et al, 2013) and L. soesokkakensis (Park et al, 2013b). At the same time, Yoon et al (2013) reported the presence of PE in L. salsilacus whereas PE was not found in this bacterium in the study of Tsubouchi et al (2013).…”

“…The genus Loktanella was expanded to include twelve more species, including Loktanella hongkongensis (Lau et al, 2004), L. agnita, L. rosea (Ivanova et al, 2005), L. koreensis (Weon et al, 2006), L. atrilutea (Hosoya & Yokota, 2007), L. maricola (Yoon et al, 2007), L. pyoseonensis (Moon et al, 2010), L. tamlensis (Lee, 2012), L. cinnabarina (Tsubouchi et al, 2013), L. litorea (Yoon et al, 2013), L. sediminilitoris (Park et al, 2013a) and L. soesokkakensis (Park et al, 2013b). Here we report the polyphasic characterization of a Gramstain-negative, aerobic, beige-pigmented, non-motile bacterium, designated KMM 9530 T , which was isolated from a shallow sediment sample.…”

Loktanella marina sp. nov., isolated from seawater

“…Subsequently, the names Loktanella hongkongensis (Lau et al, 2004), Loktanella agnita and Loktanella rosea (Ivanova et al, 2005), Loktanella koreensis (Weon et al, 2006), Loktanella atrilutea (Hosoya & Yokota, 2007), Loktanella maricola (Yoon et al, 2007), Loktanella pyoseonensis (Moon et al, 2010), Loktanella tamlensis (Lee, 2012), Loktanella cinnabarina (Tsubouchi et al, 2013), Loktanella litorea , Loktanella sediminilitoris (Park et al, 2013), Loktanella maritima (Tanaka et al, 2014), Loktanella ponticola (Jung et al, 2014), Loktanella soesokkakensis (Park et al, 2014a), Loktanella variabilis (Park et al, 2014b) and Loktanella sediminum (Liang et al, 2015) have been validly published. In this study, we describe a bacterial strain, designated MDM-7 T , which was isolated from seawater collected at Taean of the Yellow Sea in South Korea.…”