Logarithmic Growth of Protein Films

Alaeddine, Simon; Nygren, Håkan

doi:10.1021/bk-1995-0602.ch003

Cited by 15 publications

(11 citation statements)

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“…One reason may be that adsorption occurs in clusters, leading to a local enrichment of Env trimers, which would allow for bivalent binding and thus affect K d calculation. 37 Together, these data enable us to conclude that binding patterns of particulate and soluble Envs are comparable and that Env does not suffer affinity loss during either attachment procedure.…”

Section: Discussionmentioning

confidence: 63%

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Thalhauser

Peterhoff

Wagner

et al. 2020

Journal of Pharmaceutical Sciences

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Inducing immune responses protecting from HIV infection or at least controlling replication poses a huge challenge to modern vaccinology. An increasingly discussed strategy to elicit a potent and broad neutralizing antibody response is the immobilization of HIV's trimeric envelope (Env) surface receptor on a nanoparticulate carrier. As a conceptual proof, we attached an Env variant (BG505 SOSIP.664) to highly stable and biocompatible silica nanoparticles (SiNPs) via site-specific covalent conjugation or nonspecific adsorption to SiNPs. First, we demonstrated the feasibility of SiNPs as platform for Env presentation by a thorough characterization process during which Env density, attachment stability, and antigenicity were evaluated for both formulations. Binding affinities to selected antibodies were in the low nanomolar range for both formulations confirming that the structural integrity of Env is retained after attachment. Second, we explored the recognition of SiNP conjugates by antigen presenting cells. Here, the uptake of Env attached to SiNPs via a site-specific covalent conjugation was 4.5-fold enhanced, whereas adsorbed Env resulted only in a moderate 1.4-fold increase compared with Env in its soluble form. Thus, we propose SiNPs with site-specifically and covalently conjugated Env preferably in a high density as a promising candidate for further investigations as vaccine platform.

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Section: Discussionmentioning

confidence: 63%

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Thalhauser

Peterhoff

Wagner

et al. 2020

Journal of Pharmaceutical Sciences

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“…It has been reported that proteins do not distribute randomly on particle surfaces, but form clusters in association with the first proteins that bind, which in turn attracts other proteins and continues until surface is filled (18). Therefore, mechanisms such as molecular relaxation and spreading depending on the time that proteins remain on the surface, can be a determining factor in making permanent attachment of the proteins.…”

Section: Resultsmentioning

confidence: 99%

Sea Urchin Extracellular Proteins Design a Complex Protein Corona on Titanium Dioxide Nanoparticle Surface Influencing Immune Cell Behavior

et al. 2019

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Extensive exploitation of titanium dioxide nanoparticles (TiO2NPs) augments rapid release into the marine environment. When in contact with the body fluids of marine invertebrates, TiO2NPs undergo a transformation and adhere various organic molecules that shape a complex protein corona prior to contacting cells and tissues. To elucidate the potential extracellular signals that may be involved in the particle recognition by immune cells of the sea urchin Paracentrotus lividus, we investigated the behavior of TiO2NPs in contact with extracellular proteins in vitro. Our findings indicate that TiO2NPs are able to interact with sea urchin proteins in both cell-free and cell-conditioned media. The two-dimensional proteome analysis of the protein corona bound to TiO2NP revealed that negatively charged proteins bound preferentially to the particles. The main constituents shaping the sea urchin cell-conditioned TiO2NP protein corona were proteins involved in cellular adhesion (Pl-toposome, Pl-galectin-8, Pl-nectin) and cytoskeletal organization (actin and tubulin). Immune cells (phagocytes) aggregated TiO2NPs on the outer cell surface and within well-organized vesicles without eliciting harmful effects on the biological activities of the cells. Cells showed an active metabolism, no oxidative stress or caspase activation. These results provide a new level of understanding of the extracellular proteins involved in the immune-TiO2NP recognition and interaction in vitro, confirming that primary immune cell cultures from P. lividus can be an optional model for swift and efficient immune-toxicological investigations.

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“…This is because protein adsorption on a polymer surfaces is dynamically influenced by the surface chemistry of the underlying substrate. 8,48,49 The evaluation of adsorption kinetics was based on surface hydrophobicity 50 in which the initial kinetics of the adsorption reaction suggested favorable interactions between adsorbed and bulk molecules in solution. The initial adsorption phase, described as a time-limiting exponential growth, indicates that the growth kinetics is initially linear at low Fn concentrations (first order) but then undergoes a strong autocatalytic growth at high concentrations.…”

Section: Fn Adsorption On Pdmsmentioning

confidence: 99%

Fibronectin adsorption on surface‐activated poly(dimethylsiloxane) and its effect on cellular function

Toworfe

Composto

Adams

et al. 2004

J Biomedical Materials Res

141

111

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This article reports that surface modification of poly(dimethylsiloxane) (PDMS) influences fibronectin (Fn) adsorption and enhances cell attachment. Controlled adsorption of Fn on chemically activated polymer substrates is known to influence cellular function. Thin films of PDMS were spun cast on silicon wafers to obtain homogeneous and molecularly smooth surfaces. The films were made hydrophilic by exposure to ultraviolet ozone activation (PDMS*). The films then were characterized by contact angle goniometry, ellipsometry, atomic force microscopy (AFM), Rutherford backscattering spectrometry and X-ray photoelectron spectroscopy. Contact angle measurements indicated higher hydrophobicity of the nonactivated PDMS substrates than PDMS*. AFM scans of the substrates indicated higher surface roughness of PDMS* (Ra = 0.55 nm) than PDMS (Ra = 0.25 nm). Although Fn surface density (Gamma) was slightly higher on PDMS than on PDMS*, due to hydrophobic interactions between substrate and Fn, cell function was greatly enhanced on the Fn-coated PDMS* (PDMS*-Fn) than on PDMS (PDMS-Fn). Higher attachment of MC3T3-E1 osteoblast-like cells was observed on PDMS*-Fn than on PDMS-Fn. Moreover, cell spreading and cytoskeleton organization after 72 h was clearly favored on the Fn-coated PDMS* surfaces.

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Logarithmic Growth of Protein Films

Cited by 15 publications

References 0 publications

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Sea Urchin Extracellular Proteins Design a Complex Protein Corona on Titanium Dioxide Nanoparticle Surface Influencing Immune Cell Behavior

Fibronectin adsorption on surface‐activated poly(dimethylsiloxane) and its effect on cellular function

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