Locustatachykinin I and II, two novel insect neuropeptides with homology to peptides of the vertebrate tachykinin family

Schoofs, Liliane; Holman, G. Mark; Hayes, Timothy K.; Nachman, Ronald J.; Loof, Arnold De

doi:10.1016/0014-5793(90)80601-e

Cited by 216 publications

(72 citation statements)

References 26 publications

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“…Because adult mosquitoes feed on vertebrate blood, it was postulated that these sialokinins probably function in the animal's host(s) as vasodilators by interacting with vertebrate-type tachykinin receptors (Champagne and Ribeiro 1994). By means of an HPLC-based purification strategy, the first true insect TK/TRPs were identified from L. migratoria brain-retrocerebral complex-SOG extracts and were termed "locustatachykinins" or Lom-TKs (Schoofs et al 1990a(Schoofs et al , 1990b. These Lom-TKs were isolated and identified based on their myotropic properties on the L. maderae hindgut, and they were shown to stimulate contractions of the L. migratoria foregut, midgut, and oviduct.…”

Section: Tachykinin-related Peptidesmentioning

confidence: 99%

Peptidergic control of food intake and digestion in insects¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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Like all heterotrophic organisms, insects require a strict control of food intake and efficient digestion of food into nutrients to maintain homeostasis and to fulfill physiological tasks. Feeding and digestion are steered by both external and internal signals that are transduced by a multitude of regulatory factors, delivered either by neurons innervating the gut or mouthparts, or by midgut endocrine cells. The present review gives an overview of peptide regulators known to control feeding and digestion in insects. We describe the discovery and functional role in these processes for insect allatoregulatory peptides, diuretic hormones, FMRFamide-related peptides, (short) neuropeptide F, proctolin, saliva production stimulating peptides, kinins, and tachykinins. These peptides control either gut myoactivity, food intake, and (or) release of digestive enzymes. Some peptides exert their action at multiple levels, possibly having a biological function that depends on their site of delivery. Many regulatory peptides have been physically extracted from different insect species. However, multiple peptidomics, proteomics, transcriptomics, and genome sequencing projects have led to increased discovery and prediction of peptide (precursor) and receptor sequences. In combination with physiological experiments, these large-scale projects have already led to important steps forward in unraveling the physiology of feeding and digestion in insects.Key words: insect, midgut, digestion, neuropeptides, stomatogastric nervous system. Résumé : Comme tous les organismes hétérotrophes, les insectes requièrent un contrôle strict de leur ingestion de nourriture et la digestion de leur nourriture en nutriments afin de maintenir leur homéostasie et de compléter leurs tâches physiologiques. L'alimentation et la digestion sont gouvernées par des signaux tant externes qu'internes qui sont transmis par un multitude de facteurs régulateurs, livrés ou bien par les neurones qui innervent le tube digestif ou les pièces buccales ou alors par les cellules endocrines du tube digestif moyen. Notre revue fournit une synthèse des régulateurs peptidiques connus pour contrôler l'alimentation et la digestion chez les insectes. Nous décrivons la découverte et le rôle fonctionnel de ces processus dans le cas des peptides allatorégulateurs, des hormones diurétiques, des peptides apparentés aux amides FMRF, du neuropeptide F (court), de la proctoline, des peptides stimulateurs de la production de salive, des kinines et des tachykinines des insectes. Ces peptides contrôlent la myoactivité du tube digestif, l'ingestion de nourriture et(ou) la libération des enzymes digestives. Certains peptides agissent à de multiples niveaux, possédant une fonction biologique qui dépend de leur site d'action. Plusieurs peptides régulateurs ont été extraits physiquement de différentes espèces d'insectes. Cependant, plusieurs projets de peptidomique, de protéomique, de transcriptomique et de génomique ont mené à de nombreuses découvertes supplémentaires et des prédict...

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Section: Tachykinin-related Peptidesmentioning

confidence: 99%

Peptidergic control of food intake and digestion in insects¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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“…Peptidomics is a relatively new direction in proteomics research that covers the gap between proteomics and metabolomics and overlaps with both areas. However, early contributions to this field in terms of novel peptide identifications and characterizations were made from experiments on invertebrates, such as locust, fruit fly, and flesh fly, even before the term peptidomics was invented (21,22). Peptidomics methodologies are generally based on separating complex endogenous peptide mixtures by LC-usually nanoscale capillary reversed-phase LC (nanoLC; 23,24), multistep chromatographic approaches (25,26), or gel-or liquid-based isoelectric focusing (27 )-combined with MS to identify peptides.…”

Section: Neuropeptidomicsmentioning

confidence: 99%

Neuropeptidomics: MS Applied to the Discovery of Novel Peptides from the Brain

Svensson¹,

Sköld²,

Nilsson³

et al. 2007

Anal. Chem.

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“…Tachykinins were initially identified in locusts as peptides that display a certain degree of homology with vertebrate neurokinins such as substance P. 49 All these peptides display a certain degree of homology at their C-terminus (-FXGXRamide). They have been immunolocalized in the gut and are also very abundant in the brain.…”

Section: Tachykinin Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

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Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis.

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Locustatachykinin I and II, two novel insect neuropeptides with homology to peptides of the vertebrate tachykinin family

Cited by 216 publications

References 26 publications

Neuropeptidomics: MS Applied to the Discovery of Novel Peptides from the Brain

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

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