Locomotor activity in Aedes aegypti with different insecticide resistance profiles

Abstract

OBJECTIVE: To evaluate locomotor activity in four field populations of Ae. aegypti with different insecticide resistance profiles from the state of São Paulo for two years. METHODS: This study comprised the susceptible Rockefeller strain and four populations from São Paulo, Brazil: two considered populations with “reduced susceptibility” to pyrethroids (Campinas and Marília), and two “resistant populations” (Santos and Ribeirão Preto). First, 2016 and 2017 eggs from these five populations were hatched in laboratory. Virgin females underwent experiments under laboratory conditions at 25°C, with 12:12h light/dark (LD) photoperiod; 24-hour individual activity was recorded using a locomotor activity monitor (LAM). RESULTS: In females from 2016 field populations, both resistant populations showed significant more locomotor activity than the two reduced susceptibility populations and the Rockefeller strain (p < 0.05). As for females from 2017 field populations, reduced susceptibility populations showed a significant increased locomotor activity than the Rockefeller strain, but no significant difference when compared to Santos resistant population (p > 0.05). CONCLUSIONS: Our results indicate that insecticide-resistant Ae. aegypti populations show increased locomotor activity, which may affect the transmission dynamics of their arboviruses.

Simulation Analysis and Physiological and Biochemical Evaluation of Sophora Flavescens Aboveground Against Aphids Using Network Pharmacology

“…(B) were reared at low density. The photoperiod was set at 12:12 (light:dark) (Colizzi et al 2021;Nakazato et al 2021). M. persicae grown on pakchoi and mandala were used as the experimental insects.…”

“…Uniform M. persicae were selected for the experiment. The crude extract of the aboveground part of Sophora avescens, matrine, oxymatrine, sophocarpine, and sophoridine (95% purity) (Chengdu Desite Biotechnology Co., Ltd.) were dissolved in acetone solution containing 20% (Colizzi et al 2021;Nakazato et al 2021). The compounds were tested at 100 μM, 1 mM, 10 mM, and 100 mM to determine the activity of G6PD, TS, and TSPO using a kit (Jiangsu enzyme industry Co., Ltd) (Ma et al 2020).…”