Lock-In Amplified Fluorescence Spectroscopy in a Digital Microfluidic Configuration for Antibiotic Detection of Ciprofloxacin in Milk

Eswar, Rahul; Brodie, C. Harrison; Collier, Christopher M.

doi:10.1109/jmems.2022.3199167

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…In Eswar et al [42] we present the actuation and logistical methods for using a digital microfluidic platform (i.e., droplet-based microfluidics utilizing electrowetting-on-dielectric EWOD) for actuation of dairy milk, but do not explore sensing of antibiotics. In Eswar et al [43], we explore a basic digital microfluidic device for application to dairy testing. This digital microfluidic system includes a lock-in amplifier in its signal-flow diagram.…”

Section: Introductionmentioning

confidence: 99%

Lock-in Amplifier Dairy Sensor for Detection of Ciprofloxacin

et al. 2023

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This work introduces a sensor for dairy antibiotic detection of ciprofloxacin in milk. We provide an important tool for antibiotic detection in milk and the main contributions of this work are as follows. We introduce a technique based on florescence spectroscopy and lock-in amplification for sensing ciprofloxacin in milk that is capable of detection below the regulatory limit. We compare the system against one without the integrated lock-in amplification, as in traditional fluorescence sensors. We provide microchip capillary electrophoresis results and place our work in context for future integration with microchip capillary electrophoresis.

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Section: Introductionmentioning

confidence: 99%

Lock-in Amplifier Dairy Sensor for Detection of Ciprofloxacin

et al. 2023

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“…This article presents technical details for recent advancements at the University of British Columbia in lock-in detection and microfluidic devices for the dairy industry 1,2 .…”

Section: Introductionmentioning

confidence: 99%

A lock-in amplifier biosensor for dairy applications

Devasagayam,

Leclerc,

Richardson

et al. 2023

Organic and Hybrid Sensors and Bioelectronics XVI

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Antibiotic exposure can cause the development of antibiotic resistant bacteria and can induce allergic reactions in humans. A source of high antibiotic exposure is contaminated dairy milk. To prevent contaminated dairy milk, development of antibiotic biosensors for on-site detection is required. This is particularly important for dairy farmers as fines and suspension of license are consequences of shipping contaminated milk to processing plants, where antibiotic tests are currently performed. There are also environmental and economic consequences when whole dairy tanks are contaminated and go to waste. Our work addresses this problem by developing an antibiotic biosensor for farmers to test their milk onsite for ciprofloxacin prior to sending to processing plants. Ciprofloxacin is frequently used to treat common bacterial infections in cattle. Our work provides the following contributions. We introduce an antibiotic biosensor that integrates fluorescence spectroscopy, microfluidic processing, and lock-in amplification to improve the limit-of-detection of ciprofloxacin below the regulatory limit for milk. We also perform traditional fluorescence detection for comparison. Our antibiotic biosensor has a signal-flow starting with an ultraviolet light emitting diode for illumination of ciprofloxacin, and moving through a microfluidic platform, a photodiode for detection of the fluorescent wavelength, and a lock-in amplifier. Our antibiotic biosensor is well-suited for fast on-site analyses and is designed for ease-of-use. Overall, our work shows promise for the integration of real-time on-site antibiotic detection of antibiotics in dairy.

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