Location of the genes for cytochrome f, subunit IV of the b6/f complex, the α-subunit of CF1 ATP-synthase and subunit III of the CFα ATP-synthase on the barley chloroplast genome

Oliver, Richard P.

doi:10.1007/bf02908683

Cited by 7 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The corresponding position on the physical map (taken from [31]) is located in the center of the large single-copy region (Fig. 3) between the loci for the CI and E subunits of the ATPase [46]. From the hybridization data obtained with the other probes (data not shown), map positions were also deduced for the 32000 M , protein and the Ph80 chlorophyll a apoprotein genes.…”

Section: Resultsmentioning

confidence: 99%

The light‐dependent accumulation of the P700 chlorophyll a protein of the photosystem I reaction center in barley

Kreuz

Dehesh

Apel

1986

European Journal of Biochemistry

View full text Add to dashboard Cite

The light‐dependent accumulation of the P700 chlorophyll a protein of the photosystem I reaction center has been studied in greening barley (Hordeum vulgare L.) seedlings. Immunoblot analysis of total cellular protein fractions and immunogold labelling of the P700 chlorophyll a protein in ultrathin sections of Lowicryl‐embedded leaf tissue revealed that the concentration of this chlorophyll‐binding protein in plastids of dark‐grown barley seedlings is below the limit of detection. Upon illumination with white light, a rapid accumulation of this protein is induced. This light effect seems not to be regulated at the level of transcription. The gene for the P700 chlorophyll a protein has been mapped within the large single‐copy region of the plastid DNA of barley. High levels of transcripts of this gene are present already in dark‐grown seedlings and remain fairly constant throughout an extended illumination period. Polysomes were isolated from etioplasts and chloroplasts. The same high relative concentration of mRNA encoding the P700 chlorophyll a protein was present in both polysome fractions. This result suggests that the light‐dependent accumulation of the P700 chlorophyll a protein during chloroplast formation in barley seedlings is regulated at the translational, or posttranslational, level.

show abstract

Section: Resultsmentioning

confidence: 99%

The light‐dependent accumulation of the P700 chlorophyll a protein of the photosystem I reaction center in barley

Kreuz

Dehesh

Apel

1986

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…The gene for cytochromefwas subsequently located in chloroplast DNA from spinach by hybrid select translation and cell-free coupled transcription-translation (Alt et al 1983) and in wheat chloroplast DNA by coupled transcription-translation (Willey et al 1984b). The gene has also been localised in chloroplast DNA from Viciafaba (Ko et al 1984), Nicotiana tabacum (Lin andKung 1984, Shinozaki et al 1986), barley (Oliver 1984), tomato (Phillips 1985), Glycine max (Singh et al 1985), Vigna radiata, Pelargonium hortorum, Coriandrum sativum (Palmer 1985), Capsicum annuum (Gounaris et al 1986), Oenothera hookeri (Tyagi and Herrmann 1986), Ginkgo biloba (Palmer and Stein 1986), rice (Wu et al 1986), lettuce and Barnadesia (Asteraceae) (Jansen and Palmer 1987) by Southern hybridisation with heterologous probes from the pea or spinach genes. In the E. coli cell-free coupled transcription-translation system, pea, spinach and wheat cytochromefwere all synthesised as higher molecular weight precursors larger than the mature polypeptide (Alt et al 1983, Willey et al 1983.…”

Section: Genes For Components Of the Cytochrome B-f Complexmentioning

confidence: 99%

“…The genes, petB and petD, for the cytochrome b-563 and subunit IV polypeptides of the complex have been localised in chloroplast DNA from spinach (Alt et al 1983), pea (Phillips and Gray 1984b, Courtice et al 1985, Berends et al 1986), barley (Oliver 1984), wheat (Courtice et al 1985), tomato (Phillips 1985), Vigna radiata, Pelargonium hortorum, Coriandrum sativum (Palmer 1985), Capsicum annuum (Gounaris et al 1986), tobacco (Shinozaki et al 1986), Ginkgo biloba (Palmer and Stein 1986), Viciafaba (Michalowski et al 1987), lettuce, Barnadesia (Jansen and Palmer 1987) and maize (Rock et al 1987). The genes were first located in spinach chloroplast DNA by hybrid select translation and coupled transcription-translation of cloned chloroplast DNA restriction fragments (Alt et al 1983).…”

Section: Genes For Cytochrome B-563 and Subunit IVmentioning

confidence: 99%

Synthesis and assembly of the cytochrome b-f complex in higher plants

Willey

Gray

1988

Photosynth Res

View full text Add to dashboard Cite

The cytochrome b-f complex is composed of four polypeptide subunits, three of which, cytochrome f, cytochrome b-563 and subunit IV, are encoded in chloroplast DNA and synthesised within the chloroplast, and the fourth, the Rieske FeS protein, is encoded in nuclear DNA and synthesised in the cytoplasm. The assembly of the cytochrome b-f complex therefore requires the interaction of subunits encoded by different genomes. A key role for the nuclear-encoded Rieske FeS protein in the assembly of the complex is suggested by a study of cytochrome b-f complex mutants. The assembly of individual subunits of the complex may be regulated by the availability of prosthetic groups. The genes for the chloroplast-encoded subunits and cDNA clones for the Rieske FeS protein have been isolated and characterised. Cytochrome f and the Rieske FeS protein are synthesised initially with N-terminal presequences required for their correct assembly within the chloroplast. The deduced amino acid sequences of the four subunits have been used to suggest models for the arrangement of the polypeptides in the thylakoid membrane.

show abstract