Location of Neutralizing Epitopes on the Fusion Protein of Newcastle Disease Virus Strain Beaudette C

Abstract

SUMMARYA panel of eight neutralizing monoclonal antibodies (MAbs) against the fusion (F) protein of Newcastle disease virus (NDV) has been shown to locate a major antigenic site on the basis of competitive binding assay and additivity index studies. Five epitopes (A 1 to AS) have been located within this site on the F protein of the Beaudette C strain of NDV on the basis of cross-resistance plaque assays of MAb-resistant mutants raised against these MAbs. Epitopes A1, A4 and A5 are distinct; epitope A2 partially overlaps epitope A3. Nucleotide sequence analysis of the F genes of MAbresistant mutants showed that each predicted single amino acid substitutions ranging from amino acid residues 157 to 171 for epitope A4 and at residues 72, 78, 79 and 343 for epitopes A 1, A2, A3 and A5 respectively. These locations indicate that both the F 1 and F2 fragments are involved in the formation of a single antigenic site and suggest the involvement of extensive protein folding in the active form of this F protein.

Pathotypical Characterization and Molecular Epidemiology of Newcastle Disease Virus Isolates from Different Hosts in China from 1996 to 2005

“…Five genotype II isolates belonged to the LaSota type that corresponded to the poultry vaccines used in particular regions, and one belonged to the TEX/48 type that corresponded to the virulent NDV that emerged in the United States in 1948. For genotype III, there was a better relationship between the three isolates and the typical virulent strain F48E9 found in China in 1946 (30) than between the three isolates and strains with the same genotype as that of Australia-Victoria (Australia) or MIY/51 (Japan).…”

Sequence analysis of the gene encoding the fusion glycoprotein of pneumonia virus of mice suggests possible conserved secondary structure elements in paramyxovirus fusion glycoproteins

“…2 in Scopes et al, 1990). These residues include the N terminus of the F2h region and the cysteine-containing potential loop of variable length described earlier, and they correspond to regions in which neutralizing monoclonal antibodies select escape-mutations in NDV and parainfluenza virus 3 (Toyoda et al, 1988;Neyt et al, 1989;Yusoff et al, 1989;Coelingh & Tierney, 1989).…”

Hemagglutinin-Neuraminidase-Independent Fusion Activity of Simian Virus 5 Fusion (F) Protein: Difference in Conformation between Fusogenic and Nonfusogenic F Proteins on the Cell Surface

“…Our present data thus suggest that the putative F1-F2 interaction in the WR F protein involves the amino-terminal region of F2 and the middle region of F1 that includes the HR3 domain. Interestingly, a topological interrelationship between F1 (HR1 domain or cysteine-rich region) and F2 (middle region) of the Newcastle disease virus F protein has been suggested by studies on neutralization escape mutants (35,48,55) or on temperature-sensitive mutants and the revertants (59).…”