Location of myostatin expression during bovine myogenesis in vivo and in vitro

Deveaux, V.; Picard, Brigitte; Bouley, J.; Cassar-Malek, Isabelle

doi:10.1051/rnd:2004003

Cited by 26 publications

(19 citation statements)

References 47 publications

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“…The current data demonstrated that myocyte proliferation was decreased with increasing expression of myostatin by aging. Our findings support previous reports indicating that aging-induced increase in myostatin expression caused the suppression of myocyte proliferation [30]. …”

Section: Discussionsupporting

confidence: 93%

Aerobic Exercise Affects Myostatin Expression in Aged Rat Skeletal Muscles: A Possibility of Antiaging Effects of Aerobic Exercise Related With Pelvic Floor Muscle and Urethral Rhabdosphincter

Jeong

Kim

et al. 2014

Int Neurourol J

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PurposeAging-induced loss of muscle mass and subsequent reduction of strength is a fundamental cause of frailty, functional decline, and disability. And this may lead to muscular dysfunction, voiding dysfunction, or urinary incontinence due to pelvic muscle weakness induced by aging. Physical exercise has been recommended for the prevention and the treatment of these age-related frail states. We investigated the effects of treadmill exercise on muscle strength, myostatin mRNA and protein expression, and gastrocnemius myocytes proliferation in aged rats to investigate the possible antiaging effects of aerobic exercise on skeletal muscles such as pelvic floor muscles and urethral rhabdosphincter muscle.MethodsIn this study, 5-month-old male Sprague-Dawley rats were used as the young-age group (n=20) and 24-month-old rats were used as the old-age group (n=20). Each group was randomly divided into two groups (n=10 in each group): the sedentary and the treadmill exercise group. The rats in the exercise groups were forced to run on a motorized treadmill for 30 minutes, once a day, for 6 weeks. For this study, a weight load test, hematoxylin and eosin staining, real-time and reverse transcription polymerase chain reaction for myostatin mRNA, myostatin western blot, and 5-bromo-2'-deoxyuridine immunohistochemistry were performed in the gastrocnemius muscle.ResultsThe age-induced reduction of muscle mass and strength was associated with a decrease in myocyte proliferation and an increase in myostatin mRNA and protein expression in the gastrocnemius. However, treadmill exercise improved muscle mass and strength through suppression of myostatin mRNA and protein expression, and myocyte proliferation increase in the gastrocnemius against the aging process.ConclusionsAerobic exercise is a useful strategy for enhancing muscle function against aging-induced loss of skeletal muscle mass and functions.

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Section: Discussionsupporting

confidence: 93%

Aerobic Exercise Affects Myostatin Expression in Aged Rat Skeletal Muscles: A Possibility of Antiaging Effects of Aerobic Exercise Related With Pelvic Floor Muscle and Urethral Rhabdosphincter

Jeong

Kim

et al. 2014

Int Neurourol J

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“…In fact Liu et al [20] have shown that GH had an inhibitory effect on myostatin expression. In our model, we showed that GHR mRNA level increased from 130 dpc mainly in double-muscled animals and, in another study, that myostatin mRNA expression (which was very high at 110 dpc) decreased considerably during the second third of gestation both in normal and double-muscled animals [22]. The increase in GHR mRNA was perhaps due to an increase in foetal GH or of the GH-like level.…”

Section: Discussionsupporting

confidence: 59%

“…One of the objectives of the present study was to investigate the ontogeny of GHR mRNA and to compare it to that of IGF-II [8] and myostatin [22] mRNA previously described in the same samples in our laboratory. In addition, in order to better understand the regulation of the differentiation of muscle fibres, we chose an hypertrophied muscle in double-muscled animals, the semitendinosus (ST) muscle.…”

Section: Introductionmentioning

confidence: 99%

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Listrat

Hocquette²,

Picard³

et al. 2005

Reprod. Nutr. Dev.

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-The expression of the growth hormone receptor (GHR) gene was investigated in semitendinosus muscle during bovine foetal development in both normal and double-muscled Charolais foetuses which differ with respect to muscle development. Northern-blot analysis of foetal muscle RNA preparations with a GHR cDNA probe identified the 4.5 kb GHR mRNA as early as 130 days post-conception. In double-muscled animals, the expression of GHR mRNA increased from 130 to 210 days of gestation while it stayed stable in normal ones. It was significantly higher (P < 0.05) in double-muscled foetuses compared to normal ones from the second third of gestation. Northern-blot analysis of foetal muscle RNA preparations from both genotypes with a β-actin cDNA probe, revealed lower β-actin gene expression in double-muscled foetuses than in normal ones, suggesting a delay in the differentiation of muscle cells. In situ hybridisation revealed the localisation of specific GHR mRNA in muscle cells at all gestation stages analysed (130, 170, 210 days postconception) but not in connective tissue surrounding the muscle cells. At the adult stage, the hybridisation signal was also very high and observed in muscle cells only. These results show the ontogeny of GHR mRNA in bovine muscle and demonstrate a difference between normal and doublemuscled animals. muscle / bovine / receptor / growth hormone / in situ hybridisation

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“…The properties of DM muscles appear during foetal development (Picard et al, 1995;Deveaux et al, 2001). MSTN expression is detected from 16 days in bovine embryos (Kambadur et al, 1997) and is regulated throughout gestation (Deveaux et al, 2003). An increase in the total number of fibres has been observed in DM muscles during the foetal period (Swatland and Kieffer, 1974;Gagniè re et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Myostatin inactivation induces a similar muscle molecular signature in double-muscled cattle as in mice

Chelh

Picard

Hocquette

et al. 2011

Animal

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Myostatin (MSTN), a member of the TGF-b superfamily, is a negative regulator of skeletal muscle mass. We have previously shown that the cell survival/apoptosis pathway is a downstream target of MSTN loss-of-function in mice through the regulation of the expression or abundance of many survival and apoptotic factors. In this study, we used western-blot and quantitative PCR (qPCR) analyses to validate these novel downstream targets of MSTN in double-muscled (DM) cattle v. their controls including 260-day-old foetuses and adult cows from the INRA95 strain. MSTN loss-of-function in DM foetuses and DM cows resulted in a glycolytic shift of the muscles (e.g. upregulation of H-MyBP, PGM1 and SNTA1 and downregulation of H-FABP), activation of cell survival pathway through regulation of some components of the PI3K/Akt pathway (e.g. upregulation of DJ-1 and Gsk-3bser9/ Gsk-3btotal ratio and downregulation of PTEN) and upregulation of cell survival factors translationally controlled tumour protein (14-3-3E, Pink1). We also found a lower abundance of pro-apoptotic transcripts and/or proteins (Caspase-3, caspase-8, caspase-9, BID, ID2 and Daxx) and a higher expression of anti-apoptotic transcripts (Traf2 and Bcl2l2) in DM muscles. All together, these results are in favour of activation of the cell survival pathway and loss of apoptosis pathway within the muscles of DM animals. Alteration of both pathways may increase myonuclear or satellite cell survival, which is crucial for protein synthesis. This could contribute to muscle hypertrophy in DM foetuses and DM cows.Keywords: myostatin, cattle, muscle hypertrophy, apoptosis, cell survival ImplicationsMuscle hypertrophy has been extensively studied in meatproducing animals. In double-muscled (DM) cattle, a loss-offunction mutation in the myostatin gene (MSTN ) is responsible for muscle hypertrophy. In order to get a thorough knowledge of the molecular mechanisms of MSTN action, we have examined the molecular phenotype of DM muscle based on our previous data in MSTN-null mice. The data bring new elements to the understanding of the DM muscle phenotype and of the regulation of muscle mass in farm animals.

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Location of myostatin expression during bovine myogenesis in vivo and in vitro

Cited by 26 publications

References 47 publications

Aerobic Exercise Affects Myostatin Expression in Aged Rat Skeletal Muscles: A Possibility of Antiaging Effects of Aerobic Exercise Related With Pelvic Floor Muscle and Urethral Rhabdosphincter

Aerobic Exercise Affects Myostatin Expression in Aged Rat Skeletal Muscles: A Possibility of Antiaging Effects of Aerobic Exercise Related With Pelvic Floor Muscle and Urethral Rhabdosphincter

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Myostatin inactivation induces a similar muscle molecular signature in double-muscled cattle as in mice

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