Location of ?-amylase sequences in wheat and its relatives

Sharp, P. J.; Kreis, Martin E.; Shewry, Peter R.; Gale, M. D.

doi:10.1007/bf00303966

Cited by 425 publications

(146 citation statements)

References 15 publications

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“…Genomic DNA was extracted from the seedlings of the F 2:3 family using the CTAB protocol (Sharp et al 1988). The DNA was quantified with a UV spectrophotometer, and diluted to a final concentration of 30 ng/µl.…”

Section: Methodsmentioning

confidence: 99%

QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8

Wang¹,

Li²,

Shi³

et al. 2015

CZECH J GENET PLANT

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Wang J., Li Z., Shi L., Zhu L., Ren Z., Li X., Liu D., Shah S.J.A. (2015): QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8. Czech J. Genet. Plant Breed., 51: 79-85.The wheat cultivar Weimai 8 is a good source of adult-plant resistance to leaf rust in China. In order to understand the genetic background of the resistance genes, 179 F 2:3 families derived from the cross Weimai 8 and Zhengzhou 5389 (susceptible to leaf rust) were used to construct a linkage map. Results showed that there was a major QTL on chromosome 2AS, temporarily named QLr.hbau-2AS, responsible for the resistance. QLr.hbau-2AS from the resistant parent Weimai 8 was between the SSR markers Xcfd36 and Xbarc1138, with an interval length of 2.58 cM. In the 2010-2011, 2011-2012 and 2012-2013 crop seasons, it explained 25.79, 71.55 and 60.72% of the phenotypic variation, respectively. The location of QLr.hbau-2AS was close to the leaf rust resistance gene Lr37. However, they may not be the same since QLr.hbau-2AS has a different virulence test response from Lr37. This study identified two closely linked flanking molecular markers QLr.hbau-2AS and these molecular markers will help marker-assisted selection in breeding resistant cultivars in the future.

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Section: Methodsmentioning

confidence: 99%

QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8

Wang¹,

Li²,

Shi³

et al. 2015

CZECH J GENET PLANT

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“…Genomic DNA of Th. ponticum and Chinese Spring wheat was extracted separately using the method by Sharp et al (1988). Genomic DNA of Th.…”

Section: Gish Analysismentioning

confidence: 99%

“…ponticum derivate line 1-27 and its parents following the method by Sharp et al (1988). Total 1210 primer pairs were used to characterize the genomic composition of the wheat-Th.…”

Section: Molecular Marker Analysismentioning

confidence: 99%

Identification of a novel wheat-Thinopyrum ponticumaddition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers

Li¹,

Hu²,

Hu³

et al. 2015

Cereal Research Communications

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“…Total genomic DNA of the parents and F 2 single plants was extracted from leaf material of individual plants using the CTAB protocol (Sharp et al, 1988). According to results of estimation of grain color on the F 2 and F 2-3 plants, the genomic DNA of 241 recessive lines were selected and used for molecular mapping.…”

Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Locus R-D1 Conferring Red-Grain-Color in Synthetic Derivative Wheat Chuanmai 42 Mapped with SSR Markers

Li¹,

HuiTing²,

Hu³

et al. 2010

mpb

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Synthetic hexaploid wheat (SHW) (Triticum durum×Aegilops tauschii) holds a large number of unfavorable traits, of which, red grain color was important to limit the wheat breeder using SHWs for common wheat improvement. The objectives of this study were to identify and map the red grain color gene derived from SHW in a Chinese wheat variety Chuanmai 42 by SSR molecular markers. 1015 individuals in F 2 population derived from the cross of Chuanmai 42 with a red grain color and ChuanW565 (CW565) with white grain color were used for tagging the gene of red grain color. A total of 40 SSR markers on the chromosome 3D were employed to detect the polymorphism of between the parents, Chuanmai 42 and CW565. 241 recessive individuals in F 2 population derived from the cross (Chuanmai 42×CW565) were employed for allelic test of the red-grain gene by eight polymorphic markers. The results indicated that the red-grain color gene of Chuanmai 42 derived from the D genome donor of synthetic hexaploid wheat and controlled by a single dominant gene R-D1 on the long arm chromosome 3D within an interval of 4.6 flanked by SSR markers Xgwm3 and Xgwm314, and genetic distance were 2.1 cM, 2.5 cM, respectively. Those two SSR markers can be used in marker-assisted selection for developing new white grain wheat variety based on the progenies of SHWs or Chuanmai 42 crossed with white grain color wheat in wheat breeding program.

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Location of ?-amylase sequences in wheat and its relatives

Cited by 425 publications

References 15 publications

QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8

QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8

Identification of a novel wheat-Thinopyrum ponticumaddition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers

Locus R-D1 Conferring Red-Grain-Color in Synthetic Derivative Wheat Chuanmai 42 Mapped with SSR Markers

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