Location and severity of UVB irradiation damage in the rat lens

Wu, Kaili; Shui, Ying Bo; Kojima, Masami; Murano, Hidekazu; Sasaki, Kohsuke; Hockwin, O.

doi:10.1016/s0021-5155(97)00078-6

Cited by 14 publications

(7 citation statements)

References 19 publications

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“…The inbred Brown-Norway rat was chosen as the pigmented strain because it is probably the most widely used pigmented rat strain in UVR eye research, and comparison information is therefore available (Wegener 1994;Wu et al 1997). The non-pigmented Fischer-344 is also inbred and matches the growth curve of the Brown-Norway.…”

Section: Discussionmentioning

confidence: 99%

Impact of iris pigment and pupil size in ultraviolet radiation cataract in rat

Löfgren

Michael

Söderberg

2012

Acta Ophthalmologica

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ABSTRACT.Purpose: To investigate the effect of iris pigment and pupil size in ultraviolet radiation (UVR)-induced cataract. Methods: Brown-Norway rats (pigmented) and Fischer-344 rats (non-pigmented) were unilaterally exposed in vivo to 5 kJ ⁄ m 2 UVR. Each strain was split into two groups, each receiving either mydriatic (tropicamide) or miotic (pilocarpine) eye-drops. One week after exposure, the degree of ocular inflammation and damage in the anterior segment was determined. The lenses were extracted, photographed and the degree of forward light scattering (cataract) was quantified. Results: The cataract types differed between the two strains. All Fischer rats developed macroscopically identifiable UVR cataract while only 41% of Brown-Norway rats did so. All groups except the miotic Brown-Norway developed significant light scattering. The Fischer rats developed 3-4-fold more lens light scattering than the Brown-Norway rats. The miotic Fischer group exhibited significantly more light scattering than the mydriatic Fischer group. There was no significant difference in light scattering between the two Brown-Norway groups. There was a correlation between ocular inflammation and degree of light scattering, with Brown-Norway rats exhibiting less inflammation and lens light scattering. Conclusions: Pigmented rats develop less UVR cataract and less ocular inflammation than non-pigmented rats. Pupil size plays a smaller role in UVR cataract development in pigmented rats than in non-pigmented. The role of UVR-induced ocular inflammation in cataract development is still ambiguous.

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Section: Discussionmentioning

confidence: 99%

Impact of iris pigment and pupil size in ultraviolet radiation cataract in rat

Löfgren

Michael

Söderberg

2012

Acta Ophthalmologica

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“…The source of UV-B was the same as that used previously [9]. We used ten 20-watt lamps with peak wavelength at 312 nm; UV-B intensity was 0.15 mW/cm 2 at the level of the animals' eyes.…”

Section: Uv-b Irradiationmentioning

confidence: 99%

“…The eyeballs were dissected and fixed in ethanol/acetic acid (3:1 v/v) for at least 24 h. Through an anterior approach, the lens was removed. The lens epithelium was peeled off under a dissecting microscope, stained with hematoxylin, propidium iodide, and TUNEL technique [13] and observed under a light microscope or a fluorescence microscope [9]. The corneal specimens were processed for light microscopic evaluation after hematoxylin and eosin staining.…”

Section: Histological Studymentioning

confidence: 99%

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Wu¹,

Kojima²,

Shui³

et al. 2004

Ophthalmic Res

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To evaluate ocular morphological response to ultraviolet B (UV-B) exposure in guinea pigs fed with normal and low-ascorbic acid (ASA) diets, 24 guinea pigs were randomly divided into UVR (normal diet + UV-B), LVC (low-ASA diet + UV-B) and CTL (normal diet, without UV-B) groups. The irradiated animals were exposed to 8.2 J/cm² of UV-B over 29 weeks. Changes in the cornea and lens were examined using a Scheimpflug camera, specular microscope and histological studies. ASA contents in the plasma, aqueous humor and lens sections were measured by high-performance liquid chromatography. The results showed low contents of ASA in the plasma, aqueous humor and lens sections in LVC. Both irradiated groups displayed corneal epithelial edema, neovascularization in stroma, increase in corneal thickness, decrease in endothelial density, and lens epithelial damage. No significant morphological differences in cornea and lens were found between guinea pigs fed with low-ASA or normal diet.

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“…Cells were seeded in 60 mm plates (Fisher Scientific, USA) at a density of 5x10 5 cells/ml with fresh complete culture medium, and incubated for 24 h in a CO 2 incubator. Cells were grown to 70-80% confluence and then serum-starved overnight in DMEM medium prior to UV (30 mJ/cm 2 ), and collected at 4 h. Prior to UVB irradiation, cells were treated with DPI (10 μM) or EGCG (100 μg/ml) for 1 h. After treatment, the cells were washed with cold PBS. The cells were then fixed with 3.7% formaldehyde/PBS at room temperature for 5 min and washed with cold PBS, incubated with 800 μl of 3% BSA/0.3% NP40/PBS for 30 min, at room temperature, washed twice with PBS for 15 min, and incubated with anti-NF-κB p65 for 1 h at 25˚C.…”

Section: Uv Light Apparatusmentioning

confidence: 99%

“…Among the multiple factors affecting cataractogenesis, ultraviolet B irradiation (UVB) has received much attention, as the lens is regarded as an accumulating site of oxidative insults from radiant energy, including photo-oxidation by UVB (1,2). The notion that UV radiation could result in the lens opacification has been supported by accumulating epidemiologic and animal studies (3,4).…”

Section: Introductionmentioning

confidence: 99%

UVB radiation induces human lens epithelial cell migration via NADPH oxidase-mediated generation of reactive oxygen species and up-regulation of matrix metalloproteinases

Yao

Liu²,

Wang³

et al. 2009

Int J Mol Med

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Abstract. Ultraviolet (UV) radiation is one of the important cataract risk factors. The migration of human lens epithelial cells (HLECs) plays a crucial role in the remodeling of lens capsule and cataract formation. The purpose of the present study was to investigate the molecular mechanisms of UVinduced lens cell migration. We found that UVB radiation induces cell migration in cultured human lens epithelial cells. Further, we observed that UVB radiation induces NADPH oxidase activity and ROS generation which are inhibited by NADPH oxidase inhibitor diphenylene iodonium or DPI and antioxidant epigallocatechin gallate (EGCG). In addition, DPI and EGCG also block UVB irradiation-induced MMP-2, and -9 activity and expression and nuclear translocation of NF-κB. Collectively, our data suggest that NADPH oxidase may be a major source for the UVB-induced ROS generation, and it plays an essential role in the activation of NF-κB, which is involved in the activities of MMP-2 and MMP-9 and cell migration induced by UVB in HELCs. Understanding the cell signaling pathways may constitute potential therapeutic targets in for UVB-induced cataract.

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Location and severity of UVB irradiation damage in the rat lens

Cited by 14 publications

References 19 publications

Impact of iris pigment and pupil size in ultraviolet radiation cataract in rat

Impact of iris pigment and pupil size in ultraviolet radiation cataract in rat

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

UVB radiation induces human lens epithelial cell migration via NADPH oxidase-mediated generation of reactive oxygen species and up-regulation of matrix metalloproteinases

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