Location and nucleotide sequence of the gene for cytochrome b-559 in wheat chloroplast DNA

Hird, Sean M.; Willey, David L.; Dyer, Tristan A.; Gray, John C.

doi:10.1007/bf00330389

Cited by 38 publications

(13 citation statements)

References 34 publications

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“…The cytochrome b-559 encoding genes psbE and psbF of barley are located at equivalent positions on the chloroplast DNA as in spinach and other dicotyledones (5) as well as in wheat (13). The positions of the genes in the large single copy region adjacent to the gene for cytochrome fare indicated on the physical map of barley chloroplast DNA illustrated by SOGAARD and v.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleotide sequences of the psbE and psbF genes of barley are identical with those of wheat (13) and are more than 95% similar with those of spinach, tobacco and Oenothera (5). Likewise, the non-coding regions of the sequence 5' upstream of psbE and 3' downstream of psbF in barley are almost identical to those available in wheat.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, the non-coding regions of the sequence 5' upstream of psbE and 3' downstream of psbF in barley are almost identical to those available in wheat. Only one "C" in position 451 ( Figure 6) is exchanged by a "G" in wheat (13).…”

Section: Discussionmentioning

confidence: 99%

“…Hitherto, only the well-known 9 kD protein has been detected in isolated photosystem II preparations (2,18). Meanwhile the psbE-and psbF genes have been sequenced in Oenothera hookeri, Nicotiana tabacum (5) and wheat (13). More than 95% conservation of the nucleotide sequences is observed among the four species.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

Krupinska

Berry-Lowe

1988

Carlsberg Res. Commun.

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The psbE and psbF genes encoding the 9.4 and 4.4 kD apoproteins of cytochrome b-559 have been located in the chloroplast genome of barley. As in other plant species they are found adjacent to each other in the large single copy region of the chloroplast DNA. Both the nucleotide sequence and the deduced amino acid sequence for the two polypeptides are identical to that of wheat and more than 95% similar to those of spinach, tobacco and Oenothera. The region between the two genes spans 10 nucleotides (excluding the stopcodon) and contains a typical procaryotic ribosomal binding site. A dicistronic transcript is identified, but the presence of a ribosomal binding site between the two genes may allow independent translation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

Krupinska

Berry-Lowe

1988

Carlsberg Res. Commun.

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“…Eco RI and Alu I fragments were isolated from a 4.2 kbp Bam HI fragment (B12) derived from the plasmid pTac 5.44 [17] by agarose and polyacrylamide gel electrophoresis and purified by phenol extraction and ethanol precipitation before ligation into M13 mpl8 and mpl9 [17]. Isolation of single-stranded DNA and sequencing by the dideoxynucleotide chain 143 termination method was carried out according to Sanger et al [291.…”

Section: Nucleotide Sequencingmentioning

confidence: 99%

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

et al. 1989

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The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.

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Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II.

1988

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The genes encoding the two subunits (alpha and beta) of the cytochrome b559 (cyt b559) protein, psbE and psbF, were cloned from the unicellular, transformable cyanobacterium, Synechocystis 6803. Cyt b559, an intrinsic membrane protein, is a component of photosystem II, a membrane‐protein complex that catalyzes photosynthetic oxygen evolution. However, the role of cyt b559 in photosynthetic electron transport is yet to be determined. A high degree of homology was found between the cyanobacterial and green plant chloroplastidic psbE and psbE genes and in the amino acid sequences of their corresponding protein products. Cartridge mutagenesis techniques were used to generate a deletion mutant of Synechocystis 6803 in which the psbE and psbF genes were replaced by a kanamycin‐resistance gene cartridge. Physiological analyses indicated that the PSII complexes of the mutant were inactivated. We conclude that cyt b559 is an essential component of PSII.

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Location and nucleotide sequence of the gene for cytochrome b-559 in wheat chloroplast DNA

Cited by 38 publications

References 34 publications

Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

Characterization and in vitro expression of the cytochromeb-559 genes of barley I. Localization and sequence of the genes

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II.

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