Locating human splenic capillary sheaths in virtual reality

Steiniger, Birte; Wilhelmi, Verena; Berthold, Moritz; Guthe, Michael; Lobachev, Oleg

doi:10.1038/s41598-018-34105-3

Cited by 12 publications

(26 citation statements)

References 39 publications

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“…Being a highly vascular structure, the spleen appears spongy and reddish-purple in color. An adult human spleen is roughly the size of a clenched fist, although its size and weight vary, measuring about 10-12 cm (3.9-4.7 inch) vertically in the longest dimension while weighing 150-200 grams on average [1,2]. It is the only lymphoid organ that lies directly in the path of the blood circulation.…”

Section: Introductionmentioning

confidence: 99%

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Post-splenectomy Sepsis: A Review of the Literature

Tahir¹,

Ahmed²,

Malik³

2020

Cureus

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The spleen is an intraperitoneal organ that performs vital hematological and immunological functions. It maintains both innate and adaptive immunity and protects the body from microbial infections. The removal of the spleen as a treatment method was initiated from the early 1500s for traumatic injuries, even before the physiology of spleen was properly understood. Splenectomy has therapeutic effects in many conditions such as sickle cell anemia, thalassemia, idiopathic thrombocytopenic purpura (ITP), Hodgkin's disease, and lymphoma. However, it increases the risk of infections and, in some cases, can lead to a case of severe sepsis known as overwhelming post-splenectomy infection (OPSI), which has a very high mortality rate. Encapsulated bacteria form a major proportion of the invading organisms, of which the most common is Streptococcus pneumoniae. OPSI is a medical emergency that requires prompt diagnosis (with blood cultures and sensitivity, blood glucose levels, renal function tests, and electrolyte levels) and management with fluid resuscitation along with immediate administration of empirical antimicrobials. OPSI can be prevented by educating patients, vaccination, and antibiotic prophylaxis. This article summarizes the anatomy and physiology of the spleen and highlights its important functions. It primarily focuses on the pathophysiology of OPSI, its current management, and prevention strategies.

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Section: Introductionmentioning

confidence: 99%

“…In 1826, Quittembaum performed splenectomy in a 22-year-old woman for the removal of a large splenic tumor. In 1893, Jaboulay coined the 1 1 2…”

Section: Introductionmentioning

confidence: 99%

Post-splenectomy Sepsis: A Review of the Literature

Tahir¹,

Ahmed²,

Malik³

2020

Cureus

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“…a. Healing in general We routinely use our opticalflow-based interpolation [67] to reduce the anisotropy of the data in our reconstructions [99,101]. Having initially acquired the data with spatial resolution of 0.5 µm/voxel × 0.5 µm/voxel × 7 µm/voxel, we produce volumes with 0.5 µm/voxel × 0.5 µm/voxel × 1 µm/voxel for the actual 3D reconstruction.…”

Section: Healing Of Separate Channelsmentioning

confidence: 99%

“…In practice, the final meshes were visualized in our virtual-reality-based application [66]. With the ability to place annotations and perform mesh painting, our software is used by the domain experts for knowledge discovery [99][100][101]. Fig.…”

Section: Further Processing and Visualizationmentioning

confidence: 99%

“…The interaction of the experts with the model, the so-called visual analytics, is the actual way of finding insights and producing results. We developed a virtual realty application for this sake [66,99,101]. This paper, however, focuses on the cases when not everything works well in the required biomedical processing and the above 3D reconstruction pipeline.…”

Section: Introductionmentioning

confidence: 99%

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The tempest in a cubic millimeter: Image-based refinements necessitate the reconstruction of 3D microvasculature from a large series of damaged alternately-stained histological sections

Lobachev

2019

Preprint

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This work presents two methods that facilitate a 3D reconstruction of microscopic blood vessels in the volume slightly larger than 1 mm 3 . The source of the data are histological serial sections, i. e., microscopic images of probes, stained with immunohistochemistry. Odd and even sections have different stainings in our primary data set. Thus, firstly, an approach to register an alternately-stained series is presented. With image filtering and a feature-detection-based registration we obtain a registered stack of 148 serial sections. The series has missing sections, locally damaged sections, artifacts from acquisition. All these hinder correct connectivity of blood vessels. With our second approach we interpolate the missing information while maintaining the connectivity. We achieve this with deformations based on dense optical flow. The presented methodology is applicable to further histological series. A combination of both approaches allows us to reconstruct more than 76 % larger volumes. An important detail was the composition mode of images. Summarizing, we use methods from image processing and computer vision to create large-scale 3D models from immunostained histological serial sections.

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