Locating distinct objects within a thick scattering medium
remains
a long-standing challenge in the fields of materials science, health,
and engineering. Transmission Raman spectroscopy (TRS) with the use
of surface-enhanced Raman scattering (SERS) nanoparticles has proven
to be an effective approach to detect deep-seated lesions inside thick
biological tissues. However, it has not yet been proven to spatially
locate deep lesions in three dimensions using optical modalities.
Herein, we present the concept of tomographic TRS and report its successful
use for accurately locating SERS nanoparticles in elongated rod-like
thick tissues. Our work starts with theoretical simulations of Raman
photon propagation in tissues. We discovered a linear relationship
between the Raman spectral peak ratio and propagation distance of
Raman photons in tissues, allowing us to predict the location of lesions
tagged by SERS NPs. Based on this, we propose a two-step tomographic
TRS strategy, which includes axial scanning and ring scanning. We
demonstrate the robustness of our approach using ex vivo thick tissue (4.5 cm in thickness) and locate an embedded SERS phantom
lesion, with a ring scanning step of 10–30°. We successfully
locate multiple SERS phantom lesions in the ex vivo porcine muscle stack with high accuracy (absolute error of <2
mm). Our method is rapid, efficient, and of low cost compared to current
tomographic medical imaging techniques. This work advances Raman techniques
for three-dimensional positioning and offers new insights toward practical
diagnosis applications.