Locally administrated single-dose teriparatide affects critical-size rabbit calvarial defects: A histological, histomorphometric and micro-CT study

Abstract

ObjectiveThe aim of this study was to evaluate the effect of teriparatide (PTH 1-34, rhPTH) on a rabbit defect model with local xenogen grafts histomorphometrically and radiologically.MethodsFor this purpose, two 10 mm diameter critical-size defects were created in the calvaria of 16 rabbits. In the control group, the defect area was filled with a xenogen graft, while in the teriparatide group (PTH 1-34), a xenogen graft combination with 20 mcg teriparatide was used. For both 4 – week and 8 – week study groups, new bone, residual graft, and soft tissue areas were evaluated as well as bone volume histomorphometrically and radiologically.ResultsHistomorphometrically, there was a significant difference in new bone area values at the 8th week (p < 0.05), but there was no significant difference between the 4 – week values (p > 0.05). There was no statistically significant difference between the groups at both 4 and 8 weeks (p > 0.05). In the radiologically measured total bone volume values, PTH1-34 group values were found to be significantly higher for both 4 – and 8 – weeks values compared to the control groups (p < 0.05).ConclusionIn this study, rhPTH, which is used locally in defect areas to be repaired with bone grafts, increases both new bone volume and total bone volume.

The Local Release of Teriparatide Incorporated in 45S5 Bioglass Promotes a Beneficial Effect on Osteogenic Cells and Bone Repair in Calvarial Defects in Ovariectomized Rats

“…Studies have shown that the local use of teriparatide intermittently and subcutaneously resulted in an increase in the amount of newly formed bone tissue [ 38 , 56 ]. Ozer et al [ 42 ] also addressed the local use of teriparatide with xenografts in rabbits and found that in periods of 4 and 8 weeks, the group with the presence of the drug showed higher mean values of new bone formation. However, these values were greater in the period of 8 weeks.…”

“…Because of the association between bioglass particles and local medicines for osteoporosis in the in vivo response, the choice of this drug was due to its favorable effects on bone tissue metabolism [ 39 , 40 , 42 , 43 ] and evidence in the literature on its effect on cell cultures [ 44 , 45 ].…”

Effects of local single dose administration of parathormone on the early stages of osseointegration: A pre‐clinical study

“…45 However, PTH systemic administration drawbacks include the need for daily/weekly self-injections, the exposure of not-target areas, and mild side-effects. 46 Although, literature is scarce regarding the effects of local application of PTH, a handful of pre-clinical studies in calvaria 34,[47][48][49] and femoral defects 33 of small rodents have offered interesting results for bone regeneration with the association of PTH and bone grafting materials. Özer et al 48 reported that the association of xenografts with 20 μg of PTH produced a significant increase in new bone formation in critical calvaria defects at 8 weeks regarding defects treated only with xenografts.…”

“…46 Although, literature is scarce regarding the effects of local application of PTH, a handful of pre-clinical studies in calvaria 34,[47][48][49] and femoral defects 33 of small rodents have offered interesting results for bone regeneration with the association of PTH and bone grafting materials. Özer et al 48 reported that the association of xenografts with 20 μg of PTH produced a significant increase in new bone formation in critical calvaria defects at 8 weeks regarding defects treated only with xenografts. Kajii et al 47 reported similar results, with the combination of an octacalcium phosphate and collagen composite with 1.0 or 0.1 μg of PTH, where histomorphometric analysis evidenced enhanced bone regeneration in calvaria critical defects with the use of PTH compared to the control group, regardless of PTH concentration.…”