Localized Surface Plasmon Resonance as a Tool to Study Protein Corona Formation on Nanoparticles

Abstract

It is now well-accepted that nanoparticles (NPs) introduced into a biological environment will interact with the available proteins that deposit on their surface in a process known as protein corona (PC) formation which control NP interactions with cells and biological barriers. Several investigations have been conducted to understand the mechanisms and kinetics of PC formation. Among the model nanoprobes are gold (Au) NPs that possess unique optical and electromagnetic properties due to their surface plasmon resonance. These properties make Au NPs excellent probes for studying PC formation. In this Review, we describe techniques and approaches that a researcher interested in investigating PC on Au NPs may utilize in order to characterize the PC formation.

Protein-structure-dependent spectral shifts of near-infrared photoluminescence from locally functionalized single-walled carbon nanotubes based on avidin–biotin interactions

“…Notably, the increase in the f ( η 2 ) values evidences the NAV adsorption on lf-SWCNTs-b because it is reported that the estimated f ( η 2 ) values were 0.338 and 0.510 for D 2 O ( η D2O = 1.328) and the proteins ( η protein = ∼1.6 54,55 ), respectively. Focusing on the absolute values for Δ E 11 * and Δ E 11 (|Δ E 11 *| and |Δ E 11 |, respectively), the |Δ E 11 *| value was higher than the |Δ E 11 | value.…”

“…Negative-value signals are observed for ΔE 11 * and ΔE 11 because of the red-shifts of the PL peaks upon the introduction of the NAV. These values decreased further as the NAV concentration increased and eventually reached saturation at 250 nM.Notably, the increase in the f (η 2 ) values evidences the NAV adsorption on lf-SWCNTs-b because it is reported that the estimated f (η 2 ) values were 0.338 and 0.510 for D 2 O (η D2O = 1.328) and the proteins (η protein = ∼1.654,55 ), respectively.Focusing on the absolute values for ΔE 11 * and ΔE 11 (|ΔE 11 *| and |ΔE 11 |, respectively), the |ΔE 11 *| value was higher than the |ΔE 11 | value. This indicates the selective adsorption of NAV on the biotin-doped sites of lf-SWCNTs-b.…”

Photothermal Reshaping of One-Dimensional Plasmonic Polymers: From Colloidal Dispersion to Living Cells

“…28 Therefore, the investigation of physical chemistry perspectives through spectroscopic techniques and theoretical models to characterize the thermoplasmonic characteristics is indispensable to augment the conception from the colloidal dispersion inside the living cells. 29 In this article, we have demonstrated a thermoplasmonic footstep toward the in vitro photothermal reshaping of onedimensional plasmonic polymers upon laser-induced melting in confined media like a cellular environment. Five different sets of plasmonic polymers obtained through interdigitation of size-selective constituent monomers have been internalized, and subsequent photothermal reshaping leads to the transformation to quasi-rod-like nanostructures with different aspect ratios.…”

“… 28 Therefore, the investigation of physical chemistry perspectives through spectroscopic techniques and theoretical models to characterize the thermoplasmonic characteristics is indispensable to augment the conception from the colloidal dispersion inside the living cells. 29 …”

Nano-size dependent protein corona formation by SARS-CoV-2 Omicron spike protein over gold nano-colloid and reversible aggregation

“…The protein corona plays a major role in forming nano-aggregates. In order to characterize the aggregation of the bio-nano particles, understanding the conformation ofa forming protein corona is invaluable to design new bio-nano-materials [1] , [6] , [8] , [9] . A layer of protein corona was divided into two regimes: hard and soft.…”