Localized large cell lymphoma cured by simple excision

Abstract

Diffuse large cell lymphoma is frequently localized at the time of presentation, but the aggressive natural history of the disease in most patients has suggested that extensive and intensive radiation and/or chemotherapy is necessary to control this disease. We describe a patient who has remained free of disease for 20 years after simple excision of a diffuse large cell lymphoma localized to the right cervical region. Review of the biopsy material showed characteristic histological features suggestive of an orderly growth pattern of tumor cells as well as sclerosis. The clinical course of this patient after minimal therapy emphasizes the possibility of long survival with localized large cell lymphoma and suggests that the characteristic morphologic features observed in this case should be sought in other cases, since it may imply a favourable prognosis.

Lectin binding patterns in diffuse large cell lymphoma

“…(8) The sections are then covered with PNA for 30 minutes; then mouse liver powder is sprinkled over the solution and incubated another 30 minutes. (9) After washing thoroughly with buffer (usually takes more than 5 changes of buffer-3-5 minutes each), (10) After rinsing in tap water, the sections are counterstained with methyl green, and mounted with Permount. RCA, WGA, Con A, PHA, LCA and P S A This procedure followed Steps 1 through 7 as above.…”

“…(2) (8) the sections are covered with a biotinylated lectin-PBS solution (10 pg/ml) containing 100 pg/ml of mouse liver powder for 1 hour; then mouse liver powder is sprinkled over the solution, and incubated for another hour, (9) and washed in PBS, with 4 changes. (10) The sections are incubated with the ABC for 1 hour. The complex is freshly prepared by incubating 20 p1 of avidin-D ( 10 mgl ml) and 20 pl of biotin-peroxidase (3 mg/ml) in 10 ml PBS, ( 1 1) and washed in PBS, with 2 changes.…”