Localized drug delivery graphene bioscaffolds for cotransplantation of islets and mesenchymal stem cells

Abstract

A stem cell seeded graphene-dexamethasone bioscaffold significantly improves the survival and function of transplanted islets.

Long-Term Survival and Induction of Operational Tolerance to Murine Islet Allografts by Co-Transplanting Cyclosporine A Microparticles and CTLA4-Ig

“…The induction of local immunotolerance is an attractive strategy to prevent islet allograft rejection, which may improve graft function and broaden cellular therapy’s applicability while minimizing adverse side-effects of systemic immunosuppression [ 18 , 29 , 30 ]. Several strategies have been established to evoke immunoprotection by co-delivering anti-rejection drugs with particles/scaffolds to the islet grafts [ 3 , 18 , 31 , 32 , 33 ]. Immunomodulating drug-loaded microparticles provide multifaceted tools to locally modulate immune responses and represent an exciting tactic to aid cell transplantation applications.…”

Human Menstrual Blood-Derived Stem Cells Protect against Tacrolimus-Induced Islet Dysfunction via Cystathionine β-Synthase Mediated IL-6/STAT3 Inactivation

“…Each mouse was considered as a unit. To induce diabetes, mice (n = 18) were injected with 180 mg/kg streptozocin [ 10 ] (STZ, V900890, Sigma-Aldrich), which is a well-established model for inducing diabetes for studying islet transplantation [ 31 ]. Blood samples were collected from the tail vein daily for the following week to measure blood glucose levels.…”

“…Blood samples were collected from the tail vein daily for the following week to measure blood glucose levels. When nonfasting blood glucose was greater than 11.1 mmol/L for two consecutive days, the diabetic model was considered established and ready for islet transplantation [ 31 ]. Diabetic mice were euthanized using 2% isoflurane in oxygen.…”

Strategy for Clinical Setting of Co-transplantation of Mesenchymal Stem Cells and Pancreatic Islets

“…In addition, MSCs along with allograft islets were embedded in a hydrogel composite and transplanted intraperitoneally, and MSCs co-transplanted with allograft decreased pro-inflammatory cytokines 82 . Razavi et al 83 first placed bioscaffold pieces in murine internal epididymal fat pads and then co-transplanted murine islet cells co-cultured for 24 h with murine ADMSCs or murine pancreatic islet cells into the abdominal bioscaffolds. The mice with the seeded bioscaffolds achieved euglycemia by day 2 and demonstrated a significant increase in survival rate and insulin index compared with the control mice.…”