Localized and Systemic Inflammatory Mediators in a Murine Acute Mastitis Model

Takashima, Misako; Lalonde, Christian; Olszanski, Laura Ashley; Zhao, Feng‐Qi

doi:10.2147/jir.s313799

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…Blood cytokines (including MIG, MIP-1β, RANTES, G-CSF, IL-6) and tissue cytokines (including MCP-1, IL-6) were found to increase after LPS treatment in the murine acute mastitis model. 48 Consistent with previous studies, we also do not find a significant change in TNF-ɑ levels. 35 Meanwhile, TNF RII (also known as TNFRSF1B), a single-pass type I membrane protein belonging to the tumor necrosis factor (TNF) family and having a high ability to bind with tumor factor-alpha (TNF-α), was upregulated in IGM patients.…”

Section: Discussionsupporting

confidence: 93%

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Evaluation of significantly changed chemokine factors of idiopathic granulomatous mastitis in non‐puerperal patients

Li,

Nie,

Huang

et al. 2024

The FASEB Journal

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Idiopathic granulomatous mastitis (IGM), a recurrent inflammation disease of the non‐lactating breast, has had an increasing clinical morbidity rate in recent years, and its complicated symptoms and unclear etiology make it challenging to treat. This rare benign inflammatory breast disease, centered on the lobules, represents the most challenging type of non‐puerperal mastitis (NPM), also known as non‐lactating mastitis. In this study, patients diagnosed with IGM (M, n = 23) were recruited as cases, and patients with benign control breast disease (C, n = 17) were enrolled as controls. Cytokine microarray detection measured and analyzed the differentially expressed cytokine factors between IGM and control patients. Then, we verified the mRNA and protein expression levels of the significantly changed cytokine factors using Q‐RT‐PCR, ELISA, western blot, and IHC experiments. The cytokine factor expression levels significantly changed compared to the control group. We observed a significant increase between IGM and control patients in cytokine factors expression, such as interleukin‐1β (IL‐1β), monokine induced by gamma interferon (MIG), macrophage inflammatory protein (MIP)‐1α, MIP‐1β, tumor necrosis factor receptor 2 (TNF RII). Then, we verified the expression of these top five dysregulated factors in both mRNA and protein levels. Our results demonstrated the cytokine map in IGM and indicated that several cytokines, especially chemokines, were associated with and significantly dysregulated in IGM tissues compared to the control group. The chemokine factors involved might be essential in developing and treating IGM. These findings would be helpful for a better understanding of IGM and offer valuable insights for devising novel diagnostic and therapeutic strategies.

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Section: Discussionsupporting

confidence: 93%

“…It is also involved in immune regulation and inflammatory processes. Blood cytokines (including MIG, MIP‐1β, RANTES, G‐CSF, IL‐6) and tissue cytokines (including MCP‐1, IL‐6) were found to increase after LPS treatment in the murine acute mastitis model 48 …”

Section: Discussionmentioning

confidence: 98%

Evaluation of significantly changed chemokine factors of idiopathic granulomatous mastitis in non‐puerperal patients

Li,

Nie,

Huang

et al. 2024

The FASEB Journal

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“…They work antagonistically to the pro-inflammatory cytokines to prevent the exaggeration of the immune response. Il-10 decrease in the current research and previous studies was greatly involved in expanding the inflammatory response and the appearance of the disease symptoms [ 12 , 18 ].…”

Section: Discussionmentioning

confidence: 91%

Clinicopathological study on camel mastitis at Matrouh Governorate

Darwish¹

2023

J Adv Vet Anim Res

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Objective: Camel mastitis is indeed a serious problem that can have significant impacts on animal health and production as well as pose a potential public health hazard. This work aimed to identify the bacterial species responsible for camel mastitis and evaluate the associated immunological and clinicopathological alterations in infected camels. Materials and Methods: Raw milk and blood samples were collected from 40 apparently healthy she-camels, and 40 she-camels suffered from clinical mastitis (CMG). Milk samples were subjected to bacteriological examination. Serum immunological, biochemical, and hematological parameters were estimated and statistically analyzed. Results: Similar bacterial species were obtained from the two groups with different isolation rates. Staphylococcus epidermidis and Escherichia coli were the dominant species in the apparently healthy group, while Pseudomonas aeruginosa and Bacillus cereus were the dominant species in CMG. A significant ( p < 0.05) elevation of the pro-inflammatory cytokines, acute phase proteins (APPs), free radicals, total protein, Glob, kidney and liver function tests, and triglyceride concentrations were detected in CMG, and a significant ( p < 0.05) decrease in the anti-inflammatory cytokine, antioxidants, Alb, glucose, and T/LDL/HDL-cholesterol concentrations was observed in CMG. Microcytic hypochromic anemia with hypoferremia, hypotransferrinemia, hyperferritinemia, and neutrophilic leukocytosis was depicted in CMG. The estimated pro-inflammatory cytokines, APPs, and total antioxidant capacity (TAC) yielded high sensitivity and specificity, but the highest likelihood ratio was for TAC, fibrinogen (Fb), and ferritin, and the highest percentages of increase were for IL-1α and IL-1β. Conclusion: The study emphasizes the importance of hygienic preventive measures to control camel mastitis and the importance of supportive treatment to reverse the hemato-biochemicaland iron profile changes that result from the immune response in mastitic she-camels. TAC, Fb, ferritin, IL-1α, and IL-1β are good biomarkers for camel mastitis.

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“…Th1 cytokine increase has been seen in dromedaries with inflammatory and bacterial illnesses (Hussen and Schuberth 2021), as well as in clinical and SCM conditions in dairy cows and ewes (Katsafadou et al 2019;Akhtar et al 2020;Ji et al 2020;Sadat et al 2023). On the other hand, IL-10 inhibits the inflammatory immune response by preventing it from being overly exaggerated (Shaheen et al 2020;Takashima et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Impact of Age, Parity and Milking Frequency on Dromedary Camels' Susceptibility to Subclinical Mastitis

2024

Int J Vet Sci

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This study investigates the impact of age, parity, and frequency of milking on the susceptibility of three dromedary camel breeds to subclinical mastitis (SCM). The study involved 133 animals from the Al-Qassim region of Saudi Arabia, assessing systemic, milk, immunological, and bacterial characteristics, and determining the likelihood of SCM through somatic cell count (SCC) and bacterial count of milk. Blood and milk samples were analyzed for bacterial counts, somatic cell count, and immunoglobulin G (IgG), lactoferrin (LTF), and lactoperoxidase (LPO) concentrations. ELISA kits were used to quantify serum Cam-TNF-α and Cam-IL-6 Cam-IL-10 concentrations. SCC and milk examination were used to determine the tendency to form SCM. The study found a significant increase in SCC in Majahem and Shaele camels (P<0.001) after TAD milking and age >7 years (P<0.001), while a decline in Wadha camels (P<0.05). All breeds showed an increase in SCC at parity >2, with Majahem and Shaele most affected by factors like increased SCC and bacteriological examination. The study examined the impact of factors on serum IgG, LTF, and LPO in different breeds of camels. Results presented significant (P<0.05) elevations in serum IgG and LPO in Majahem, Wadha, and Shaele camels (P<0.01), while milk LTF increased significantly (P<0.05) in Shaele camels. The study found that after TAD milking, Majahem camels showed increased TNF-α (P<0.05), IL-6 (P<0.001) and IL-10 (P<0.05) levels, while Shaele camels showed a decrease in TNF-α (P<0.001) and a decline in IL-10 (P<0.05), indicating excellent indicator of udder status. The study highlights the importance of understanding microbiology, SCC, and immune parameters of milk camel breeds for hygienic practices. It highlights the need for early detection of SCM in camel milk, promoting further research to improve detection performance.

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Localized and Systemic Inflammatory Mediators in a Murine Acute Mastitis Model

Cited by 6 publications

References 55 publications

Evaluation of significantly changed chemokine factors of idiopathic granulomatous mastitis in non‐puerperal patients

Evaluation of significantly changed chemokine factors of idiopathic granulomatous mastitis in non‐puerperal patients

Clinicopathological study on camel mastitis at Matrouh Governorate

Impact of Age, Parity and Milking Frequency on Dromedary Camels' Susceptibility to Subclinical Mastitis

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