Localized and regulated peptide pigment formation inside liquid droplets through confined enzymatic oxidation

Barriales, Kenny; Kassem, Salma; Sementa, Deborah; Vidal Ceballos, Alfredo; Wang, Tong; Khandaker, Shadman; Abzalimov, Rinat R.; Jain, Ankit; Elbaum-Garfinkle, Shana; Ulijn, Rein V.

doi:10.1039/d3cc04231f

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…On the other hand, inspired by biosynthesis of eumelanin, melanin-like polymeric pigments are developed from supramolecular peptide self-assembly with adjustable supramolecular structure. Unique structures not available in nature can be synthesized by altering the sequence of self-assembled tyrosine-containing tripeptides under enzymatic oxidation. , Ulijn and co-workers prepared melanin-like fibers based on a self-assembling tripeptide made up of lysine–tyrosine–tyrosine. Two tyrosine molecules in this supramolecular structure exhibited enhanced electrical conductivity while preserving 1D morphology after oxidation by forming conductive tracks …”

Section: Introductionmentioning

confidence: 99%

Melanin-Inspired Composite Materials: From Nanoarchitectonics to Applications

Liu,

Ding,

Yuan

et al. 2024

ACS Appl. Mater. Interfaces

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Synthetic melanin is a mimic of natural melanin analogue with intriguing properties such as metal−ion chelation, redox activity, adhesion, and broadband absorption. Melanin-inspired composite materials are formulated by assembly of melanin with other types of inorganic and organic components to target, combine, and build up the functionality, far beyond their natural capabilities. Developing efficient and universal methodologies to prepare melaninbased composite materials with unique functionality is vital for their further applications. In this review, we summarize three types of synthetic approaches, predoping, surface engineering, and physical blending, to access various melanin-inspired composite materials with distinctive structure and properties. The applications of melanininspired composite materials in free radical scavenging, bioimaging, antifouling, and catalytic applications are also reviewed. This review also concludes current challenges that must be addressed and research opportunities in future studies.

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Section: Introductionmentioning

confidence: 99%

Melanin-Inspired Composite Materials: From Nanoarchitectonics to Applications

Liu,

Ding,

Yuan

et al. 2024

ACS Appl. Mater. Interfaces

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Chaos‐Assisted Production of Micro‐Architected Spheres (CAPAS)

Ceballos‐González,

Bolívar‐Monsalve,

Velásquez‐Marín

et al. 2024

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Hydrogel droplets with inner compartments are valuable in various fields, including tissue engineering. A droplet‐based biofabrication method is presented for the chaos‐assisted production of architected spheres (CAPAS) for the rapid generation of multilayered hydrogel spheres (ranging from 0.6 to 3.5 mm in diameter) at high‐throughput rates (up to 2000 spheres per min). This method is based on the use of chaotic advection generated by a Kenics static mixer (KSM) nozzle. The configuration of the KSM (i.e., the number of mixing elements) determines the number of compartments within the sphere. Sphere size is adjusted by flow rate, printhead outlet diameter, polymer concentration (sodium alginate or gelatin‐methacryloyl (GelMA)), and crosslinking bath composition. This versatile system operates in dripping and jetting modes, preserving multilayered architecture in both modes. Proof‐of‐concept experiments with breast cancer (MDA‐MB‐231), human dermal fibroblast (HDF), and murine myoblast (C2C12) lines show over 80% cell viability immediately post‐fabrication, maintained over extended culture (14 or 30 days). CAPAS is used to create a breast cancer model with cancer‐tissue‐like and healthy‐tissue‐like micro‐niches to test paclitaxel doses. It is envisioned that CAPAS will enable high‐throughput fabrication of hydrogel spheres for tissue engineering, chemical engineering, and material sciences applications.

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