Localization profiles of natriuretic peptides in hearts of pre-hibernating and hibernating Anatolian ground squirrels (Spermophilus xanthoprymnus)

Öztop, Mustafa; Özbek, Mehmet; Lïman, Narin; Beyaz, Feyzullah; Ergün, Emel; Ergün, Levent

doi:10.1007/s11259-019-9745-5

Cited by 8 publications

(7 citation statements)

References 92 publications

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“…The Anatolian ground squirrel atria were used as a positive control in order to determine the specificity of the antibodies. As seen in our previous study (Öztop et al, ), NPPA, NPPB and NPPC stainings were observed in cardiomyocytes (Figure ). For the negative control, nonimmune serum was used instead of the primary antibody and the other immunohistochemical procedures were applied in the same way.…”

Section: Resultssupporting

confidence: 85%

“…At the same time, we used nonspecific rabbit serum (sc‐2027; Santa Cruz Biotechnology) instead of primary antibodies for isotype control. Anatolian ground squirrel atria were used for positive controls, as reported in our previous study (Öztop et al, ). The same procedure was performed for all the immunostaining processing including the negative and positive controls.…”

Section: Methodsmentioning

confidence: 99%

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Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

et al. 2019

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Natriuretic peptide (NP) family is composed of atrial, brain and C-type NP (NPPA, NPPB and NPPC). Here, we aimed to investigate NP expression in testis and epididymis during postnatal development. NPPA expression was observed in gonocytes at prepubertal period but in only spermatocytes in pachytene and leptotene/ zygotene stage at pubertal period. In prepubertal and pubertal periods, we detected NPPB expression in only Leydig cells. However, NPPC expression was detected in all of the gonocytes and Sertoli cells, spermatocytes and some interstitial cells in prepubertal and pubertal periods. In postpubertal and mature periods, NPPA and NPPB staining were detected in Leydig cells, elongated and round spermatids but not in spermatogonia and spermatocytes. However, we observed NPPC expression in all cells of the seminiferous tubules and Leydig cells in the postpubertal and mature periods. Epididymal epithelium showed intense NPPC expression during postnatal period but weak NPPA and NPPB expression in prepubertal and pubertal periods.The expression of three NPs in the testis significantly increased after puberty. In conclusion, puberty had a significant effect on NP expression in testis. Unlike NPPA and NPPB, expression of NPPC in all cells of the seminiferous tubule suggests that NPPC is effective in each step of spermatogenesis. K E Y W O R D Satrial natriuretic peptide, brain natriuretic peptide, C-type natriuretic peptide, epididymis, rat, testis

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Section: Resultssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

et al. 2019

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“…Earlier studies [28][29][30] focused on the expression of ANP and its receptors in BAT, and these studies confirmed that there was a specific binding site for ANP in BAT, which was the basis for ANP to regulate BAT. Although ANP was recognized to be secreted by the heart, 76 Bae et al 32 and Öztop et al, 33 respectively, demonstrated that brown adipocytes and BAT could be stimulated to express and secrete ANP, which was consistent with the results in Figure 2 from Human Protein Atlas database.…”

Section: Discussionsupporting

confidence: 74%

“…HEK‐293 are kidney‐derived cells with ANP receptors, and Bae et al further demonstrated that ANP secreted by beige‐like adipocytes acted on receptor cells HEK‐293, leading to increased cGMP activity. Öztop et al 33 also discovered ANP expression in adipose tissue, and they further observed interesting changes in the expression levels of ANP in the heart and adipocytes before and after hibernation in Anatolian ground squirrels. By using immunohistochemistry, the authors revealed that the expression level of cardiac ANP during hibernation was lower than before hibernation.…”

Section: Resultsmentioning

confidence: 90%

Regulatory role of atrial natriuretic peptide in brown adipose tissue: A narrative review

Tian

et al. 2022

Obesity Reviews

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Summary Atrial natriuretic peptide (ANP) has been considered to exert an essential role as a cardiac secretory hormone in the regulation of hemodynamic homeostasis. As the research progresses, the role of ANP in the crosstalk between heart and lipid metabolism has become an interesting topic that is attracting the interest of researchers. The regulation of ANP in lipid metabolism shows favorable effects, particularly the activation of brown adipose tissue (BAT). The complex regulatory network of ANP on BAT has not been fully outlined. This narrative review critically evaluated the existing literature on the regulatory effects of ANP on BAT. In general, we have summarized the expression of ANP and its receptors in various human tissues, analyzed the progress of research on the relationship between the ANP and BAT, and described several potential pathways of ANP to BAT. Exogenous ANP, natriuretic peptide receptor C (NPRC) deficiency, cold exposure, bariatric surgery, and cardiac or renal insufficiency could all contribute to BAT expression by increasing circulating ANP levels.

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“…It has long been considered that BAT merely exists in some mammalian species, including human newborns, hibernating animals, and rodents [68][69][70]. However, recent studies have shown that BAT is metabolically activated in adult humans upon cold exposure [71][72][73].…”

Section: Brown Adipose Tissuementioning

confidence: 99%

Cardiac Natriuretic Peptide System: A Link between Adipose Tissue, Obesity, and Insulin Resistance

Öztop¹

2022

Evolving Concepts in Insulin Resistance

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Cardiac natriuretic peptides (NPs) play critical roles in body systems, besides essentially maintaining cardiovascular homeostasis. White adipose tissue exerts functions such as energy storage, hormone secretion, thermal insulation, regulation of insulin sensitization, and prevention of traumatic injuries to vital organs. Brown adipose tissue is a thermogenic tissue that protects the body from cold environments by dissipation of chemical energy derived from fuel substrates as heat. NPs have potent regulatory effects on adipose tissues having high expression of NP receptors. Evidence suggests that the NP system participates in the regulation of adipose tissue metabolism during obesity, insulin resistance, and type 2 diabetes. Reduced NP synthesis and changed clearance receptor expression may adversely affect NPs’ target organ metabolism during obesity, insulin resistance, and type 2 diabetes. Defective NP system might lead to adipose tissue dysfunction during obesity, type 2 diabetes, insulin resistance, and cardiovascular disease. Improved NP levels have been associated with positive metabolic outcomes. The positive association between increased NP levels and lower incidence of insulin resistance, obesity, and type 2 diabetes holds promise for future applications of NPs system in clinical settings. This chapter provides an overview of the impact of the NP system on adipose tissue metabolism in cardiometabolic diseases.

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Localization profiles of natriuretic peptides in hearts of pre-hibernating and hibernating Anatolian ground squirrels (Spermophilus xanthoprymnus)

Cited by 8 publications

References 92 publications

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

Regulatory role of atrial natriuretic peptide in brown adipose tissue: A narrative review

Cardiac Natriuretic Peptide System: A Link between Adipose Tissue, Obesity, and Insulin Resistance

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