Localization ofN-cadherin in the normal and regenerating nerve fibers of the chicken peripheral nervous system

Shibuya, Yasuyuki; Mizoguchi, Akira; Takeichi, Masatoshi; Shimada, Keikichi; Idé, Chizuka

doi:10.1016/0306-4522(95)00015-b

Cited by 79 publications

(51 citation statements)

References 32 publications

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“…In regenerating nerves, Schwann cells also precede and guide axons by expressing several cell adhesion molecules including Ncadherin for S-A interaction [Shibuya et al, 1995;Torigoe et al, 1996;Fu and Gordon, 1997]. Thus, Schwann cells play a leading role in the early stages of S-A interaction.…”

Section: Importance Of Ecm Proteins In S-a Interactionmentioning

confidence: 99%

The Role of Schwann Cell-Axon Interaction in Peripheral Nerve Regeneration

Namgung

2014

Cells Tissues Organs

112

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After peripheral nerve injury, Schwann cells are released from the degenerating nerve, dedifferentiated, and then actively participate in axonal regeneration. Dedifferentiated Schwann cells, together with macrophages, are involved in eliminating myelin debris, forming bands of Büngner that provide pathways for regenerating axons, and redifferentiating for remyelination. Activation of Erk1/2 and c-Jun was shown to induce stepwise repair programs in Schwann cells, indicating that plastic changes in Schwann cell activity contribute to interaction with axons for regeneration. Schwann cell β1 integrin was identified to mediate the Cdc2-vimentin pathway and further connect to adaptor molecules in the growth cone of regenerating axons through the binding of extracellular matrix (ECM) proteins. Timely interaction between Schwann cells and the axon (S-A) is critical to achieving efficient axonal regeneration because the delay in S-A interaction results in retarded nerve repair and chronic nerve damage. By comparing with the role of Schwann cells in developing nerves, this review is focused on cellular and molecular aspects of Schwann cell interaction with axons at the early stages of regeneration.

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Section: Importance Of Ecm Proteins In S-a Interactionmentioning

confidence: 99%

The Role of Schwann Cell-Axon Interaction in Peripheral Nerve Regeneration

Namgung

2014

Cells Tissues Organs

112

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“…Our previous study also showed that N-cadherin was expressed on the surface of axolemma at cell-cell contacts, but not at cell-extracellular matrix contacts (Shibuya et al, 1995). However, there was no distinct difference in ~N-catenin density on the plasmalemma between the cell-cell and cell-extracellular matrix contacts areas.…”

Section: Discussionmentioning

confidence: 80%

“…However, the small foci of intense immunoreactivity adjacent to the axolemma suggest that there might be some local differences in the adhesion on the plasmalemma of growth cones. Our previous study showed that some growth cone vesicles exhibited N-cadherin immunoreactivity on the inner surface of the limiting membrane (Shibuya et al, 1995). As N-cadherin is a transmembrane protein, the monoclonal antibody against N-cadherin (NCD-2) (Hatta & Takeichi, 1986) used in that study recognized an extracellular domain of the N-cadherin molecule .…”

Section: Discussionmentioning

confidence: 96%

“…Regenerating sprouts usually emanate from the nodes of Ranvier near to the lesion, and elongate distally in touch with the inner surface of basal laminae, or the plasmalemma of Schwann cells (Ide & Kato, 1990;Tomatsuri et al, 1993;Kato & Ide, 1994). These sprouts and Schwann cells express N-cadherin on the plasmalemma where they are in contact with one another (Shibuya et al, 1995), Therefore, it is likely that aNcatenin comes to be expressed in the sprouts at the nodes of Ranvier of the myelinated nerve.…”

Section: Discussionmentioning

confidence: 99%

“…We have shown that normal unmyelinated nerves express N-cadherin on the plasmalemma of both axons and Schwann cells where they are in contact with each other, while myelinated nerves do so only at the mesaxon. Moreover, in regenerating nerves, N-cadherin is expressed on the plasmalemma of axons and Schwann cells where axon-axon and axon-Schwann cell contacts are made (Shibuya et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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αN-catenin expression in the normal and regenerating chick sciatic nerve

et al. 1996

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The Ca(2+)-dependent intercellular adhesion molecule cadherin is known to be linked to the cytoskeleton by the protein catenin, an association of which appears to be important for the cell-adhesion function of cadherin. Catenin consists of three subtypes-alpha, beta, and gamma. In our previous study, N-cadherin was shown to be localized on the plasmalemma of normal and regenerating chick peripheral nerve. Thus, as alpha N-catenin is a subtype of alpha-catenin (which is specifically associated with N-cadherin), we investigated the immunolocalization of alpha N-catenin in normal and regenerating chick sciatic nerve. In normal nerve, unmyelinated axons exhibited either intense or weak alpha N-catenin immunoreactivity throughout the axoplasm, whereas myelinated axons were completely immunonegative. Regenerating axons, including those derived from parent myelinated axons, showed alpha N-catenin immunoreactivity of variable intensities in growth cones and axon shafts. Schwann cells were invariably devoid of immunoreactivity. Thus alpha N-catenin is not necessarily bound to the surface plasmalemma, but is distributed throughout the cytoplasm, suggesting that most alpha N-catenin molecules are dissociated from N-cadherin.

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Upregulation and redistribution of cadherins reveal specific glial and muscle cell phenotypes during Wallerian degeneration and muscle denervation in the mouse

et al. 1999

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In the present work, we investigated the expression of cadherin mRNAs in the adult neuromuscular system either under normal conditions or following experimental neurotomy. Cadherin-6, a marker of Schwann cell precursors, was not expressed in the adult peripheral nerve, while M-cadherin, cadherin-11, and N-cadherin were expressed both by glial and conjunctive cells. Moreover, the three transcripts were transiently upregulated in the distal stump of neurotomized sciatic nerve during Wallerian degeneration: N-cadherin was abundant in myelinating Schwann cells during myelin degradation, while M-cadherin and cadherin-11 may be upregulated in proliferating Schwann cells. M-cadherin, cadherin-11, and N-cadherin were also detected in myofibres and endomysium of adult gastrocnemius muscle. Following neurotomy, cadherin-11 was only transiently increased in denervated myofibres, while M-cadherin was increased and sustained for at least 21 days postoperation. In contrast, N-cadherin was not upregulated in denervated myofibres. Thus, we defined here a combination of cadherins expressed in the adult nerve and muscle, and modulated during Wallerian degeneration and muscle denervation. The comparison of the expression pattern of this combination of cadherins to the one previously described during embryonic development shows that chronically denervated Schwann and muscle cells do not reverse to embryonic state (recapitulative hypothesis), but present specific phenotypic features.

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Localization ofN-cadherin in the normal and regenerating nerve fibers of the chicken peripheral nervous system

Cited by 79 publications

References 32 publications

The Role of Schwann Cell-Axon Interaction in Peripheral Nerve Regeneration

The Role of Schwann Cell-Axon Interaction in Peripheral Nerve Regeneration

αN-catenin expression in the normal and regenerating chick sciatic nerve

Upregulation and redistribution of cadherins reveal specific glial and muscle cell phenotypes during Wallerian degeneration and muscle denervation in the mouse

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