Localization of uricase in mycorrhizas of Ophrys lutea Cav.

Abstract

SUM .VI A R YInfected roots of Ophrys Intea present numerous peroxisome profiles identified by diaminobenzidine (DAB) staining. The presence of uricase in these peroxisomes could also he recognized cytochemically. The activities of catalase and uricase, assayed using a crude fraction of peroxisomes, reached 0-8//kat min ' mg protein^' and 1-73 nkat min ' mg protein ' respectively. The role of uricase in purine metabolism is discussed in terms of host/ endophyte interactions in O. lutea mycorrhizas.

Unlocking the Mycorrhizal Nitrogen Pathway Puzzle: Metabolic Modelling and multi-omics unveil Pyrimidines’ Role in Maize Nutrition via Arbuscular Mycorrhizal Fungi Amidst Nitrogen Scarcity

“…Our findings highlight a metabolic interplay between the host and AMF, with pyrimidine and ureide metabolism serving as key players in this interaction (BARROSO et al, 1988;Thakur and Garg, 2023). Furthermore, the observed differences in how our two studied maize lines embrace different strategies to benefit from the fungus illustrate the importance of leveraging maize's extensive genetic diversity for a comprehensive understanding of this symbiotic interaction (Schmidt et al, 2016;Sawers et al, 2018b;Liu et al, 2020).…”

“…Physiological profiling combined with metabolic modelling revealed pyrimidine and ureide metabolism as previously unrecognized pivotal components in this interaction, warranting additional investigation 80,81 . Additionally, the contrasting strategies adopted by our two maize lines to capitalize on the fungus highlight the significance of harnessing maize's broad genetic diversity for a holistic comprehension of this symbiotic relationship 24,82,83 .…”

“…However, we did not detect variations in allantoin levels in inoculated plant roots compared to non-inoculated plants and did not detect allantoic acid (Supplementary Figure 8). Further investigations are required to elucidate the role of ureides in the nitrogen economy of the symbiotic association, as limited reports suggest mycorrhizal fungi's potential to produce ureides or induce allantoin and allantoic acid production in plants (BARROSO et al, 1988;Thakur and Garg, 2023).…”