Localization of types I, II and III collagen and glycosaminoglycans in the mandibular condyle of growing monkeys: an immunohistochemical study

Abstract

In order to analyse the regional and age-related variations of primate condyles, immunohistochemical techniques were used to examine the localization of types I, II and III collagen and a variety of glycosaminoglycans in distinct anteroposterior regions of the mandibular condyle of two growing female rhesus monkeys (Macaca mulatta). In the juvenile monkey staining for types I and III collagen was weak in the fibrous tissue layer, intense in the pre-cartilaginous tissue layer and faint in the cartilaginous tissue layer; staining was significantly more intense in the posterosuperior and posterior regions than in the anterior region. Similarly, staining for cartilage-characteristic extracellular matrices, including type II collagen and keratan sulfate, was intense in the cartilaginous tissue layer of the posterior condyle. In contrast, in the late-adolescent monkey staining for the extracellular matrices was more intense in the anterior half of the condyle (i.e. from the anterior to the posterosuperior region) than in the posterior region, and most intense in the posterosuperior region. The results demonstrate that marked regional differences exist in the phenotypic expression of the extracellular matrices in the mandibular condyles of growing monkeys and that these differences vary between different developmental stages. The variations probably reflect the predominance of competing growth and articulatory functions in the mandibular condyles.

An Immunohistochemical Study of the Localization of Types I, II and III Collagen in the Temporomandibular Joint of Growing Monkeys.

“…The mandibular condylar cartilage and glenoid fossa showed similar layer-specific staining patterns for the three types of collagen, as described previously [10,20,22,23,29], although the staining intensities decreased in the late-adolescent monkey. Interestingly, in the condylar cartilage in the late-adolescent stage, the staining for type II collagen was limited to the pericellular region and chondrocytes became small.…”

“…Collagens and proteoglycans (PG)s are prominent extracellular matrix (ECM) components in the TMJ [2,4,8,10,14,16,17,22,23,29,33]. Over 10 genetically and structurally distinct types of collagen have been found in various connective tissues, and these can be divided into two different subgroups, i.e., fibrillar and FACIT (fibril-associated collagen with interrupted triple helices) [15].…”

“…Our previous study using immunohistochemical techniques showed that the distribution of types I, II and III collagen was markedly different in the central and peripheral areas of the condylar cartilage of growing monkeys [23]. However, little information is available concerning the localization of the collagens in various regions of the monkey TMJ during growth [20].…”

Mineral Surface in Calcified Plaque Is Like That of Bone

“…Colocalization of GAGs and mineralization has been detected histologically in calcifying tissue, [12][13][14] and importantly there is accumulating evidence that the type of GAG in the matrix may exert tight control over mineralization, particularly during its initiation. 15,16 An early event in bone mineralization is release of membrane-bound matrix vesicles which act as the nidus for mineral nucleation by concentrating calcium and phosphate.…”

Análise da estrutura e padrão de expressão de lubricina, SMAD2 fosforilada na cadeia de ligação e colágeno tipo I na cartilagem articular da mandíbula durante o envelhecimento

“…O colágeno tipo II está presente apenas nas camadas madura e hipertrófica da CAM em crescimento, sendo mais intenso na primeira 56,57,59,[66][67][68]70 . A quantidade de colágeno tipo II na camada madura também varia de acordo com a região da CAM, sendo mais evidente nas partes anterior e média do que na posterior 68 .…”