Localization of tumor suppressor gene associated with distant metastasis of urinary bladder cancer to a 1-Mb interval on 8p22

Ohgaki, Kenji; Iida, Aritoshi; Ogawa, Osamu; Kubota, Yoshinobu; Akimoto, Masao; Emi, Mitsuru

doi:10.1002/(sici)1098-2264(199905)25:1<1::aid-gcc1>3.0.co;2-3

Cited by 33 publications

(22 citation statements)

References 14 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were obtained by other researchers (Li et al, 2001;Wang et al, 2001a). Besides HCC, the deletion of chromosome 8p has also been shown to play an important role in the tumor progression and metastasis of many other kinds of human malignancies, including colorectal (Takanishi et al, 1997;Parada et al, 1999), bladder (Wagner et al, 1997;Ohgaki et al, 1999;Muscheck et al, 2000), breast (Yokota et al, 1999), larynx (Kujawski et al, 1999), renal (Bissig et al, 1999), and lung (Petersen et al, 2000) cancers. Therefore, 8p might harbor one or more tumorsuppressor genes that are important in progression, especially the metastasis of cancers, including HCC (Nihei et al, 2002).…”

Section: Discussionsupporting

confidence: 83%

HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma

Wang

et al. 2005

Oncogene

View full text Add to dashboard Cite

Our previous studies suggested that chromosome 8p deletion is associated with metastasis of hepatocellular carcinoma (HCC), in which some novel metastasis suppressor genes might be harbored. The present study aimed to identify the metastatic suppressor gene(s). A cDNA chip was constructed with the expressed sequence tags (ESTs) from chromosome 8p and used to compare the difference of expression profiling between the MHCC97-H and MHCC97-L cell lines with different metastatic potentials and similar genetic backgrounds. In all, 10 ESTs were significantly downregulated in MHCC97-H cell line with higher metastatic potential. One full-length gene, HTPAP (phosphatidic acid phosphatase type 2 domain containing 1B), was identified at chromosome 8p12. Sequencing and bioinformatic analyses revealed that HTPAP has 826 bp and encodes a putative protein of 175 amino acids with a transmembrane segment at the NH2 terminus, two protein kinase C phosphorylation site and one tyrosine kinase phosphorylation site. Its expression level in metastatic tumor tissues was much lower than that of primary HCC tissues. Both in vitro and in vivo assays suggested that HTPAP could suppress the invasion and metastasis of HCC. These suggested that HTPAP is a novel metastatic suppressor gene for HCC. The mechanism of the effect of HTPAP on HCC metastasis is not clear yet and deserves further investigation.

show abstract

Section: Discussionsupporting

confidence: 83%

HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma

Wang

et al. 2005

Oncogene

View full text Add to dashboard Cite

show abstract

“…Among those cases, a 41.0cM MDR on 8p22 was found. Frequent LOH of this region has been described previously in bladder cancer (14,32,33). Homozygous deletion has also been identified in prostate cancer and medulloblastoma (34)(35)(36) suggesting that multiple TSGs are located in this region.…”

Section: Discussionmentioning

confidence: 65%

Progressive increase of genetic alteration in urinary bladder cancer by combined allelotyping analysis and comparative genomic hybridization

Chan

Hui²,

Yip³

et al. 2009

Int J Oncol

View full text Add to dashboard Cite

Abstract. Bladder cancer is the ninth most common cancer in the world. Urothelial carcinoma (formerly known as transitional cell carcinoma) comprises the majority of bladder cancers. In order to decipher the genetic alteration leading to the carcinogenesis of urothelial cancer, we performed genome-wide allelotyping analysis using 384 microsatellite markers spanning 22 autosomes together with comparative genomic hybridization (CGH) in 21 urothelial cancer. High frequency of allelic imbalance was observed in chromosome arm 1q (61.9%), 3p (61.9%), 4q (66.67%), 8p (57.14%), 9p (76.2%) and 9q (66.67%). Allelic imbalance with frequency above average was also observed in chromosome arm 2q, 10p, 10q, 11p, 11q, 12q, 13q, 15q, 17p and 19q. The allelic imbalance of each case and fractional allelic loss for each chromosome was associated with higher tumor grade and stage (P<0.05). We have also delineated several minimal deletion regions on chromosome 3p, 4q, 8p, 9p, 9q, 11p, 13q, 16q and 17p. By CGH analysis, common chromosomal alterations included gain of 1p, 1q, 12q, 16p, 17q and 19p as well as loss of 4q and 9p in most of the cases. Our findings may provide valuable information to locate putative oncogenes and tumor suppressor genes in the carcinogenesis of bladder cancer in this locality.

show abstract

“…Subregional deletion analysis of chromosome 8 in a wide spectrum of tumors demonstrates a high degree of LOH for probes within 8p22, including prostate (Bova et al 1993;Macoska et al 1995;Cunningham et al 1996), colon (Cunningham et al 1993;Takanishi et al 1997), lung Lerebours et al 1999;Wistuba et al 1999), liver Pineau et al 1999), male breast (Chuaqui et al 1995), breast (Patel et al 1994;Kerangueven et al 1997), ovarian (Wright et al 1998), and urinary bladder (Ohgaki et al 1999) cancers, although current evidence suggests that there are several discrete, nonoverlapping sites of LOH on chromosomal bands 8pter → p23, 8p22, 8p21, and 8p12 → p11. The general interpretation of these findings is that there is more than one tumor suppressor gene on 8p and additional data are needed to further narrow the critical region(s) to identify the genes (The Third International Workshop on Human Chromosome 8 mapping 1996).…”

mentioning

confidence: 99%

High-Resolution Physical Map and Transcript Identification of a Prostate Cancer Deletion Interval on 8p22

Arbieva¹,

Banerjee²,

Kim³

et al. 2000

Genome Res.

View full text Add to dashboard Cite

A genomic interval of ∼1-1.5 Mb centered at the MSR marker on 8p22 has emerged as a possible site for a tumor suppressor gene, based on high rates of allele loss and the presence of a homozygous deletion found in metastatic prostate cancer. The objective of this study was to prepare a bacterial contig of this interval, integrate the contig with radiation hybrid (RH) databases, and use these resources to identify transcription units that might represent the candidate tumor suppressor genes. Here we present a complete bacterial contig across the interval, which was assembled using 22 published and 17 newly originated STSs. The physical map provides twofold or greater coverage over much of the interval, including 17 BACs, 15 P1s, 2 cosmids, and 1 PAC clone. The position of the selected markers across the interval in relation to the other markers on the larger chromosomal scale was confirmed by RH mapping using the Stanford G3 RH panel. Transcribed units within the deletion region were identified by exon amplification, searching of the Human Transcript Map, placement of unmapped expressed sequence tags (ESTs) from the Radiation Hybrid Database (RHdb), and from other published sources, resulting in the isolation of six unique expressed sequences. The transcript map of the deletion interval now includes two known genes (MSR and N33) and six novel ESTs.

show abstract

Localization of tumor suppressor gene associated with distant metastasis of urinary bladder cancer to a 1-Mb interval on 8p22

Cited by 33 publications

References 14 publications

HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma

HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma

Progressive increase of genetic alteration in urinary bladder cancer by combined allelotyping analysis and comparative genomic hybridization

High-Resolution Physical Map and Transcript Identification of a Prostate Cancer Deletion Interval on 8p22

Contact Info

Product

Resources

About