Localization of Thioredoxin Reductase and Thioredoxin in Normal Human Placenta and Their Protective Effect Against Oxidative Stress

Ejima, Kuniaki; Nanri, Hiroki; Toki, Naoyuki; Kashimura, Masamichi; Ikeda, Masaharu

doi:10.1053/plac.1998.0338

Cited by 72 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The serum levels of oxidative stress-related molecules, such as lipid peroxides and malondialdehyde, have been found to be higher in pregnant women than in non-pregnant women [15]. The placenta, which is regarded as a source of such molecules [11], produces 4-hydroxynonenal (4-HNE) and thioredoxin (TRX) in normal pregnancies [1,3,14]. Meanwhile, maternal endothelial cell dysfunction has attracted attention as a key event in the development of the diverse clinical manifestations of preeclampsia [21], and the placenta is regarded as a major source of the factors that lead to such dysfunction in preeclampsia [20].…”

Section: Introductionmentioning

confidence: 99%

Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

et al. 2004

View full text Add to dashboard Cite

Recent evidence suggests that oxidative stress is involved in the pathophysiology of preeclampsia. Using immunohistochemistry and Western blotting, we investigated the oxidative stress- and redox-related molecules, such as 8-hydroxy-2'-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), thioredoxin (TRX) and redox factor-1 (ref-1) in the placenta in preeclampsia, intrauterine growth restriction (IUGR), preeclampsia + IUGR and in normal pregnancy. Using immunohistochemistry, the level of 8-OHdG was significantly higher in IUGR ( P=0.012) or preeclampsia + IUGR (P=0.0021) than in normal pregnancy, while TRX expression was significantly higher in preeclampsia (P=0.045), and ref-1 expression was significantly higher in preeclampsia (P=0.017), IUGR (P=0.016) and preeclampsia + IUGR (P=0.0038) than in normal pregnancy. The levels of 4-HNE did not differ significantly between either preeclampsia or IUGR and normal pregnancy. A significant positive correlation was observed between TRX and ref-1 expressions in both normal (rho=0.52) and complicated (rho=0.43) pregnancies. Using Western blotting, ref-1 expression tended to be higher in complicated pregnancies than in normal pregnancy (P=0.09). These results suggest that oxidative DNA damage is increased in IUGR and that redox function is enhanced in both preeclampsia and IUGR compared with normal pregnancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

et al. 2004

View full text Add to dashboard Cite

show abstract

“…We have reported that thioredoxin (Trx) and glutaredoxin (Grx) serve as a repair system for oxidatively damaged proteins via reduction of oxidized thiols in vascular endothelial cells (5)(6)(7). Furthermore, recent studies have shown that Trx and Trx reductase can also scavenge reactive oxygen species (8)(9)(10)(11), indicating that these proteins constitute a novel cellular defense system against oxidative injury in addition to the conventional antioxidant enzymes such as catalase, superoxide dismutase and glutathione peroxidase.…”

Section: Introductionmentioning

confidence: 99%

17beta-estradiol induces protein thiol/disulfide oxidoreductases and protects cultured bovine aortic endothelial cells from oxidative stress

Ejima

Nanri

Araki

et al. 1999

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: To examine whether or not estrogens induced the expression of protein thiol/disulfide oxidoreductases such as protein disulfide isomerase (PDI), thioredoxin (Trx), Trx reductase, and glutaredoxin (Grx) in vascular endothelial cells. Methods:The regenerative effects of the protein thiol/disulfide oxidoreductases, PDI, Trx and Grx, on oxidatively damaged proteins were assayed using H 2 O 2 -inactivated glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a reporter enzyme. The induction of protein thiol/disulfide oxidoreductases and the accumulation of protein adducts generated by lipid peroxidation were examined by Western blotting in estrogen-treated bovine aortic endothelial cells (BAECs). Results: Reduced PDI, Trx and Grx regenerated the H 2 O 2 -inactivated GAPDH in vitro. The levels of these protein disulfide oxidoreductases in BAECs were increased by pretreatment with 0.01-10 mmol/l 17b-estradiol, the largest increase (about fourfold of the control) being found for PDI. Other sex hormones such as progesterone and testosterone did not affect the contents of these oxidoreductases in BAECs. 4-Hydroxy-2-nonenal (HNE)-protein adducts, which are generated by lipid peroxidation, were accumulated in BAECs exposed to paraquat, whereas the pretreatment of BAECs with 17b-estradiol suppressed their accumulation. Conclusions:The estrogen-mediated induction of the protein thiol/disulfide oxidoreductases such as PDI, Trx, Trx reductase and Grx suggested a possible involvement of these oxidoreductases in the antioxidant protection of estrogen observed in the vascular system.

show abstract

“…It was reported that protein disulfide isomerase (PDI) serves as a repair system for oxidatively damaged proteins via reduction of oxidized thiols in vascular endothelial cells. 20) Upregulated PDI may play a critical role in resistance to ischemic damage, and the elevation of levels of this protein in the brain may have beneficial effects in brain stroke.…”

mentioning

confidence: 99%

Gastrodia elata Blume and an Active Component, p-Hydroxybenzyl Alcohol Reduce Focal Ischemic Brain Injury through Antioxidant Related Gene Expressions

Kim

Lee

et al. 2005

Biological & Pharmaceutical Bulletin

103

View full text Add to dashboard Cite

Ischaemic stroke is a leading cause of death and long-lasting disability. Gastrodia elata blume (GEB) is a Chinese herb that is widely used to treat convulsive disorders, such as epilepsy, and p-hydroxybenzyl alcohol (HBA) is the active ingredient in GEB. The present study was conducted to evaluate the effects of GEB and HBA on the brain damage and transcriptional levels of Protein disulfide isomerase (PDI) and 1-Cys peroxiredoxin (1-Cys Prx) genes known to play a role in antioxidant systems after transient focal ischemia in the rat brain. Focal ischemia was induced in rats by middle cerebral artery occlusion (MCAO). All animals underwent ischemia for 1 h, followed by 24 h of reperfusion. Coronal brain slices were stained with 2,3,5-triphenyltetrazolium chloride or total RNA was extracted for the analysis of gene expression. Histopathologic analysis revealed a significant ( pϽ0.05) decrease in infarct size in the ipsilateral brain with GEB extracts or HBA. Moreover, the levels of PDI and 1-Cys Prx transcription were significantly increased in the GEB extract-or HBA-treated group compared with the untreated group (pϽ0.05). This study therefore indicated that GEB and HBA provide neuroprotection by preventing brain damage through the increased expression of genes encoding antioxidant proteins after transient focal cerebral ischemia and may be effective as neuroprotective agents at the cellular and molecular levels in the brain.

show abstract

Localization of Thioredoxin Reductase and Thioredoxin in Normal Human Placenta and Their Protective Effect Against Oxidative Stress

Cited by 72 publications

References 0 publications

Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

17beta-estradiol induces protein thiol/disulfide oxidoreductases and protects cultured bovine aortic endothelial cells from oxidative stress

Gastrodia elata Blume and an Active Component, p-Hydroxybenzyl Alcohol Reduce Focal Ischemic Brain Injury through Antioxidant Related Gene Expressions

Contact Info

Product

Resources

About