Localization of the progesterone receptor in the porcine ovary

Słomczyńska, Maria; Krok, Małgorzata; Pierściński, A

doi:10.1078/s0065-1281(04)70027-6

Cited by 45 publications

(33 citation statements)

References 24 publications

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“…In rodent and primate ovaries, follicles that developed beyond the primary stage no longer expressed PR in granulosa cells until after the surge of LH, and the LH surge induced a rapid increase in expression of PR in granulosa cells (Hild-Petito et al 1988, Park & Mayo 1991, Shao et al 2003. In pig, early antral follicles contained PR in granulosa cells, and the stimulation of FSH increased the level in granulosa cells (Slomczynska et al 2000). In dog, Vermeirsch et al (2001) reported that the amount of PR in granulosa cells increased with the stage of follicle development, the level being significantly higher in the secondary and tertiary follicles compared with the primordial and primary follicles.…”

Section: Pr In Cumulus Cells During Meiotic Resumption Of Oocytesmentioning

confidence: 99%

“…In in vitro culture of rat granulosa cells, high concentrations of either FSH or LH stimulated PR gene expression (Natraj & Richards 1993). In pigs, Slomczynska et al (2000) showed that in granulosa cells of small antral follicles, PRs were not detected; however, the treatment of small follicles with LH or FSH in vitro induced the expression of the PR protein. Although it is known that the expression of the PR in granulosa cells is regulated by the gonadotropin surge, there has until now been no description of the ratios of PR-A to PR-B mRNA in cumulus cells surrounding oocytes during in vitro meiotic maturation of COCs.…”

Section: Introductionmentioning

confidence: 99%

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Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Shimada¹,

Yamashita²,

Ito³

et al. 2004

Journal of Molecular Endocrinology

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The present study aimed to investigate progesterone receptor (PR) gene expression in cumulus cells and their roles during meiotic resumption of porcine oocytes. The amount of PR-A or PR-B mRNA was analyzed by RT-PCR using primer sets for the PR-B region or the PR-A/B common region. The level of PR-B mRNA in cumulus cells was up-regulated by FSH and LH during the first 8 h of cultivation but the level significantly decreased at 12 h. However, a high level of total PR mRNA was maintained up to a cultivation period of 20 h. The level of PR-B protein in cumulus cells reached its maximum at 4 to 12 h, whereas PR-A predominated in cumulus cells of cumulus-oocyte complexes (COCs) at 20 h. Accompanying the shift in expression of PR isoforms, progesterone production in cumulus cells was significantly increased, and both the proliferative activity of cumulus cells during a 10-to 20-h cultivation period and the level of connexin-43, a major component of the gap junction, in cumulus cells significantly decreased. When COCs were cultured with FSH and LH for 10 h and then further cultured with additional RU486, there was a significant suppression in the shift in PR isoforms and in progesterone production, a loss of proliferative activity, and a decrease in connexin-43 mRNA in cumulus cells. Moreover, treatment with RU486 after 10-h cultivation of COCs inhibited the meiotic resumption of oocytes and cumulus cell expansion. These results suggest that the induction of PR isoforms in cumulus cells and their binding to progesterone appear to impact on proliferation and differentiation in a time-dependent manner, and the shift from PR-B to PR-A may help mediate certain events.

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Section: Pr In Cumulus Cells During Meiotic Resumption Of Oocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Shimada¹,

Yamashita²,

Ito³

et al. 2004

Journal of Molecular Endocrinology

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“…In the same period, the concentration of 20 ng/ml of progesterone in association with FSH showed an oocyte diameter larger than control and MEM + alone. The effect of progesterone in the ovary are exerted by their receptors (PRA and PRB) present in theca and granulosa cells of preantral and antral follicles in mouse (Gava et al 2004), swine (Slomczynska et al 2000) and bovine (D´Haeseleer et al 2007) and these receptors increase progressively in conformity with follicular development. However, it was demonstrated that in mouse without progesterone receptors (PRA and PRB), follicular development can normally occurs (Lydon et al 1996), suggesting that other receptors can be involved in progesterone action.…”

Section: Discussionmentioning

confidence: 99%

Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

et al. 2012

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We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a signiϐicant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a signiϐicant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies conϐirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles. tos de tecido ovariano foram cultivados por 1 ou 7 dias em Meio Essencial Mínimo (MEM) sozinho ou contendo progesterona (1, 2.5, 5, 10 ou 20ng/mL), FSH (50ng/mL) ou a combinação entre esses dois hormônios. O tecido fresco (controle não-cultivado) e o cultivado foram processados para análise histológica e ultra-estrutural. Após 7 dias a adição de FSH a todas as concentrações de progesterone manteve o percentual de folículos normais similar ao controle fresco. No dia 7 de cultivo, um alto percentual de folículos em desenvolvimento foi observado somente no tratamento com 2,5ng/ml de progesterona associada ao FSH ou com 10ng/ml de progesterona sozinha, em relação ao controle fresco. Do dia 1 para o dia 7 de cultivo, um aumento signiϐicativo no percentual de folículos em desenvolvimento foi observado no MEM sozinho e adicionado de 2,5ng/ml de progesterona + FSH. Além disso, após 7 dias, Pesq. Vet. Bras. 32(4):361-367, abril 2012 362Isabel B. Lima-Verde et al.em todos os tratamentos, houve um aumento signiϐicativo no diâmetro folicular em relação ao controle, exceto nos tratamentos com MEM sozinho, 5ng/ml de progesterona + FSH ou 10ng/ml de progesterona sozinha. A análise ultra--estrutural conϐirmou a integridade follicular após 7 dias de cultivo no tratamento com 2,5ng/ml de progesterona + FSH. Em conclusão, este estudo demonstrou que a interação entre progesterona e FSH mantém a integridade ultra--estrutural, estimula a ativação de folículos primordiais e o posterior crescimento de folículos pré-antrais caprinos cultivados ...

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“…This is mainly because of the diagnosis and treatment of infertility, safety of hormone therapy in the perimenopausal period, as well as influence on oncogenesis in a nipple, endometrium and ovary. In the ovaries of reproductive-age women, PR is observed in stromal and surface epithelial cells, in the early stages of follicular development, and in theca and granular cells [5,7,22,23]. Its localization is also well known in corpus luteum [23,24].…”

Section: Introductionmentioning

confidence: 99%

“…of LH and estradiol [6,7,15,18,19], and to smaller degree on BMI and the levels of testosterone, androstendione, DHEAS, prolactin [20,21] and progesterone [19].…”

Section: Introductionmentioning

confidence: 99%

The presence and role of progesterone receptor in the ovaries of postmenopausal women who have not applied hormone replacement therapy.

Starczewski

Brodowska²,

Piasecka³

2008

Folia Histochem Cytobiol.

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Localization of the progesterone receptor in the porcine ovary

Cited by 45 publications

References 24 publications

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

The presence and role of progesterone receptor in the ovaries of postmenopausal women who have not applied hormone replacement therapy.

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