Localization of the pannexin1 protein at postsynaptic sites in the cerebral cortex and hippocampus

Zoidl, Georg; Petrasch‐Parwez, Elisabeth; Ray, Arundhati; Meier, Carola; Bunse, Stefanie; Habbes, Hans-Werner; Dahl, Gerhard; Dermietzel, Rolf

doi:10.1016/j.neuroscience.2007.01.061

Cited by 151 publications

(137 citation statements)

References 35 publications

Supporting

Mentioning

133

Contrasting

Order By: Relevance

“…3D and E). Furthermore, immunocytochemistry and detection by light and electron microscopy revealed Panx1 in rodent hippocampal and cortical principal neurons accumulating exclusively at postsynaptic densities 17 consistent with Panx1 localizing within plasma membrane domains that are clearly separated from apposing membranes.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 53%

“…natural conditions in cells endogenously expressing Panx1 such as CNS pyramidal 17,35 and Purkinje neurons, 18 erythrocytes, [19][20][21] T-cells, 22 astrocytes, 23 airway epithelial cells, 24 taste cells 25 and cochlear supporting cells. 26 Functional properties previously attributed to connexin hemichannels may be valid, but may need to be re-addressed in light of these pannexin findings.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

See 1 more Smart Citation

Pannexin channels are not gap junction hemichannels

et al. 2011

View full text Add to dashboard Cite

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 53%

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

Pannexin channels are not gap junction hemichannels

et al. 2011

View full text Add to dashboard Cite

“…57 Interestingly, Px1 has been demonstrated to colocalize with postsynaptic density protein, PSD95, suggesting that this hemichannel may have a role in synaptic function. 58 Px1 expression has been reported in cultured astrocytes, oligodendrocytes and neurons, 59 but is absent in C6 glioma cells. 60 It has not been determined to date if Px1 is expressed in astrocytes in vivo or in brain slices.…”

Section: Pannexin Hemichannelsmentioning

confidence: 99%

Connexin and pannexin hemichannels of neurons and astrocytes

Thompson

MacVicar²

2008

Channels

View full text Add to dashboard Cite

Hemichannels are large pore ion channels that in the traditional view are formed when half a gap connexin junction opens to the extracellular space. It is now evident that other ion channel families, including the newly discovered pannexin family can form channels with all the nascent properties of hemichannels. This suggests that hemichannels should now be defined to include members of non-connexin families. Several connexin, and two pannexins are expressed in neurons and astrocytes where they may function in release of ATP and glutamate. Additionally, pannexin-1 appears to play a role in neuronal death. Hemichannels form a novel and unique class of ion channels that likely have diverse physiological and pathophysiological roles in the nervous system.

show abstract

“…7 A, B). Because pannexin-1 is enriched in the dendrites of CA1 cells (Ray et al, 2005;Zoidl et al, 2007), this finding raises the possibility that pannexins allow neurons to promote calcium elevations in nearby astrocytes through a non-vesicular release of molecules such as ATP and glutamate.…”

Section: Mapping Astrocyte Responses To Neuronal Stimulationmentioning

confidence: 99%

Astrocytes Display Complex and Localized Calcium Responses to Single-Neuron Stimulation in the Hippocampus

Bernardinelli

Salmon²,

Jones³

et al. 2011

Journal of Neuroscience

View full text Add to dashboard Cite

Astrocytes show a complex structural and physiological interplay with neurons and respond to neuronal activation in vitro and in vivo with intracellular calcium elevations. These calcium changes enable astrocytes to modulate synaptic transmission and plasticity through various mechanisms. However, the response pattern of astrocytes to single neuronal depolarization events still remains unresolved. This information is critical for fully understanding the coordinated network of neuron-glial signaling in the brain. To address this, we developed a system to map astrocyte calcium responses along apical dendrites of CA1 pyramidal neurons in hippocampal slices using single-neuron stimulation with channelrhodopsin-2. This technique allowed selective neuronal depolarization without invasive manipulations known to alter calcium levels in astrocytes. Light-evoked neuronal depolarization was elicited and calcium events in surrounding astrocytes were monitored using the calcium-sensitive dye Calcium Orange. Stimulation of single neurons caused calcium responses in populations of astrocytes along the apical axis of CA1 cell dendrites. Calcium responses included single events that were synchronized with neuronal stimulation and poststimulus changes in calcium event frequency, both of which were modulated by glutamatergic and purinergic signaling. Individual astrocytes near CA1 cells showed low ability to respond to repeated neuronal depolarization events. However, the response of the surrounding astrocyte population was remarkably accurate. Interestingly, the reliability of responses was graded with respect to astrocyte location along the CA1 cell dendrite, with astrocytes residing in the primary dendrite subregion being most responsive. This study provides a new perspective on the dynamic response property of astrocyte ensembles to neuronal activity.

show abstract

Localization of the pannexin1 protein at postsynaptic sites in the cerebral cortex and hippocampus

Cited by 151 publications

References 35 publications

Pannexin channels are not gap junction hemichannels

Pannexin channels are not gap junction hemichannels

Connexin and pannexin hemichannels of neurons and astrocytes

Astrocytes Display Complex and Localized Calcium Responses to Single-Neuron Stimulation in the Hippocampus

Contact Info

Product

Resources

About