Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells.

Abstract

The N-terminal P1 proteinase of potato virus Y (ordinary strain group isolate PVY-O) was expressed in E. coli. Antiserum was raised against the expressed protein and used to detect the viral proteins in infected tobacco leaf tissue by Western blotting and by electron microscopy with immunogold labelling. In the immunogold localization studies P1 protein was detected in association with the cytoplasmic inclusion bodies characteristic of PVY infections and in the cytoplasm of the infected plant cells. No significant P1 antibody binding with other plant cell organelles, or with the cell wall and plasmodesmata, was detected by immunogold labelling.

P1 peptidase – a mysterious protein of family Potyviridae

“…However, parallel to this information, it has also been asserted that neither of these two proteins appears to be required for cell-to-cell movement. Arbatova et al (1998) later indirectly confirmed this assertion. Immunogold localization of PVY P1 protein in infected plants showed an absence of this protein in the cell wall and plasmodesmata (Arbatova et al 1998).…”

“…Arbatova et al (1998) later indirectly confirmed this assertion. Immunogold localization of PVY P1 protein in infected plants showed an absence of this protein in the cell wall and plasmodesmata (Arbatova et al 1998). On the other hand, we need to mention the situation in the sobemoviruses.…”

“…However, no significant P1 antibody binding with other plant cell organelles, or with the cell wall and plasmodesmata, was detected with the immunogold labelling. Arbatova et al (1998) described their findings of P1 associated with pinwheel-shaped inclusion bodies with two possible explanations: (1) P1 could stay associated with this complex due to its simultaneous synthesis there as well, as it has been shown by the P3 protein (Ammar et al , 1994Rodríguez-Cerezo et al 1991. (2) As suggested by Ammar et al (1994), all potyviral proteins might by synthesized in, on or near the inclusions, which are associated with a rough endoplasmic reticulum.…”

“…, Pea seed-borne mosaic virus (PSbMV) (Aparicio et al 2005), Potato virus A (PVA) Kekarainen et al 2002), Potato virus V (PVV) (Oruetxebarria and Valkonen 2001), Potato virus Y (PVY) (Arbatova et al 1998;Pehu et al 1995), Potato virus X (PVX) (Vance et al 1995;Pruss et al 1997), Yam mosaic virus (YMV) (Aleman-Verdaguer et al 1997), Tobacco etch virus (TEV) (Carrington and Dougherty 1988;Carrington and Freed 1990;Verchot et al 1992;Verchot and Carrington 1995a, b) and Turnip mosaic virus (TuMV) (Soumounou and Laliberté 1994). On the other hand, there are those experiments concerned with the sequence variability of the P1 coding region in YMV (Aleman-Verdaguer et al 1997), PVA (Kekarainen et al 1999), PVV (Oruetxebarria et al 2000), PVY (Tordo et al 1995;Mukherjee et al 2004), ZYMV (Lee and Wong 1998;Wisler et al 1995), TVMV andTEV (Domier et al 1987), within the group of Potyviruses (Riechmann et al 1992), within the Potyviridae family (Adams et al 2005b;Valli et al 2008), or among several viral families, including Potyviridae (Chare and Holmes 2006).…”

Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection

“…6A) and the mutationally changed polarity and acidic property, respectively, of these positions implied their criticality. P1 protein's involvement in genome amplification has been suggested for Tobacco etch virus (TEV) (Verchot and Carrington 1995) and Potato virus Y (PVY) (Arbatova et al 1998). Its RNA-binding activity has been attributed to its specific structure and ionic interaction with the phosphate backbone of viral RNA (Brantley and Hunt 1993;Merits et al 1998).…”