Localization of the genes for major ribosomal RNA on chromosomes of the house musk shrew, Suncus murinus, at meiotic and mitotic cells by fluorescence in situ hybridization and silver staining.

Rogatcheva, Margarita B.; Serdyukova, Natalia A.; Biltueva, Larisa S.; Perelman, Polina L.; Бородин, П. М.; Oda, Sen-ichi; Graphodatsky, Alexander S.

doi:10.1266/ggs.72.215

Cited by 5 publications

(3 citation statements)

References 15 publications

(18 reference statements)

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“…Therefore we paid special attention to those chromosome regions where genes for 18+28S rRNA genes of S. murinus were located (Rogatcheva et al, 1997b): 5p15-pter; 9q64-qter; 13q38-qter. However, no difference in the strength of the hybridisation signals between these loci and other chromosomal termini was detected.…”

Section: Resultsmentioning

confidence: 99%

Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area.

et al. 2000

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The house musk shrew, Suncus murinus, is polymorphic for five Robertsonian translocations (Rb8.17, 9.13, 10.12, 11.16, 14.15). Fluorescence in situ hybridisation with a biotin-labelled oligonucleotide, (TTAGGG) 7 , was performed to localise the telomeric DNA sequences at Rb chromosomes of heterozygous shrews. Hybridisation signals were observed at both ends of all chromosomes, but not at the pericentromeric areas of any of the Robertsonian metacentrics. Our results indicate a complete loss of the telomeric sequences at the fusion points of the Rb metacentrics in S. murinus.

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Section: Resultsmentioning

confidence: 99%

Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area.

et al. 2000

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“…NORs have been localized at telomeres of chromosomes of several mammalian species (Ray 1979, Rogatcheva et al 1997, Liu & Fredga 1999, Rakotoarisoa et al 2000, Raudsepp et al 2000. On the other hand, studies on the distribution of telomeric repeats showed frequent non-telomeric co-localizations with NORs (Meyne et al 1990).…”

Section: Discussionmentioning

confidence: 99%

The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA

Жданова¹,

Minina²,

Karamisheva³

et al. 2007

Chromosome Res

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Two closely related shrew species, Sorex granarius and Sorex araneus, in which Robertsonian rearrangements have played a primary role in karyotype evolution, present very distinct telomere length patterns. S. granarius displays hyperlong telomeres specifically associated with the short arms of acrocentrics, whereas telomere lengths in S. araneus are rather short and homogenous. Using a combined approach of chromosome and fibre FISH, modified Q-FISH, 3D-FISH, Ag-NOR staining and TRF analysis, we carried out a comparative analysis of telomeric repeats and rDNA distribution on chromosome ends of Sorex granarius. Our results show that rDNA sequences forming active nuclear organizing regions are interspersed with the long telomere tracts of all short arms of acrocentrics. These observations suggest that the major rearrangements that gave rise to today's karyotype in S. granarius were accompanied by a profound reorganization of chromosome ends, which comprised extensive amplification of telomeric and rDNA repeats on the short arms of acrocentrics and finally contributed to the stabilization of telomeres. This is the first time that such telomeric structures have been observed in any mammalian species.

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“…Detection of ribosomal clusters DNA of a plasmid carrying human 28S ribosomal gene (Rogatcheva et al 1997) was labelled with biotin by nick translation (Invitrogen) and used in standard dual and multicolor fluorescence in situ hybridisation (FISH) experiments.…”

Section: Multidirectional Cross-species Chromosome Paintingmentioning

confidence: 99%

Chromosomal evolution in Gekkonidae. I. Chromosome painting between Gekko and Hemidactylus species reveals phylogenetic relationships within the group

et al. 2011

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Geckos are a large group of lizards characterized by a rich variety of species, different modes of sex determination and diverse karyotypes. In spite of many unresolved questions on lizards' phylogeny and taxonomy, the karyotypes of most geckos have been studied by conventional cytogenetic methods only. We used flow-sorted chromosome-specific painting probes of Japanese gecko (Gekko japonicus), Mediterranean house gecko (Hemidactylus turcicus) and flat-tailed house gecko (Hemidactylus platyurus) to reveal homologous regions and to study karyotype evolution in seven gecko species (Gekko gecko, G. japonicus, G. ulikovskii, G. vittatus, Hemidactylus frenatus, H. platyurus and H. turcicus). Generally, the karyotypes of geckos were found to be conserved, but we revealed some characteristic rearrangements including both fissions and fusions in Hemidactylus. The karyotype of H. platyurus contained a heteromorphic pair in all female individuals, where one of the homologues had a terminal DAPI-negative and C-positive heterochromatic block that might indicate a putative sex chromosome. Among two male individuals studied, only one carried such a polymorphism, and the second one had none, suggesting a possible ZZ/ZW sex determination in some populations of this species. We found that all Gekko species have retained the putative ancestral karyotype, whilst the fission of the largest ancestral chromosome occurred in the ancestor of modern Hemidactylus species. Three common fissions occurred in the ancestor of Mediterranean house and flat-tailed house geckos, suggesting their sister group relationships. PCR-assisted mapping on flow-sorted chromosome libraries with conserved DMRT1 gene primers in G. japonicus indicates the localization of DMRT1 gene on chromosome 6.

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Localization of the genes for major ribosomal RNA on chromosomes of the house musk shrew, Suncus murinus, at meiotic and mitotic cells by fluorescence in situ hybridization and silver staining.

Cited by 5 publications

References 15 publications

Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area.

Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area.

The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA

Chromosomal evolution in Gekkonidae. I. Chromosome painting between Gekko and Hemidactylus species reveals phylogenetic relationships within the group

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