Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage

Bertram, Michael J.; Neubaum, Deborah M.; Wolfner, Mariana F.

doi:10.1016/s0965-1748(96)00064-1

Cited by 118 publications

(120 citation statements)

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“…These differences may result from low-level contamination by accessory gland fragments or cells in the large-scale testes preparations for the EST project or may indicate that Acp36DE is transcribed, but not translated, in the testes. Consistent with such models, all 15 Acp antibodies thus far generated detect exclusively accessory gland-specific proteins, even though 9 of 15 genes [CG8982 (Acp26Aa), CG4605 (Acp32CD), CG7157 (Acp36DE), CG6289, CG8137, CG9334, CG17575, CG1656, CG9029] (Monsma et al 1990;Coleman et al 1995;Bertram et al 1996;Lung et al 2002;Ravi Ram et al 2005) have testis EST hits (Andrews et al 2000;Rubin et al 2000a;Parisi et al 2003).…”

Section: Methodsmentioning

confidence: 82%

“…Occasionally adult testis ESTs (Rubin et al 2000a) included our Acp candidates. For example, Acp36DE [a highly expressed Acp (Wolfner et al 1997)] has 32 testes EST hits, but has been shown by Western blots to be an accessory gland-specific protein (Bertram et al 1996;Wolfner et al 1997). These differences may result from low-level contamination by accessory gland fragments or cells in the large-scale testes preparations for the EST project or may indicate that Acp36DE is transcribed, but not translated, in the testes.…”

Section: Methodsmentioning

confidence: 99%

“…These 52 D. melanogaster ACPs are expected to be extracellular and thus transferred to the female upon mating and to be produced primarily or exclusively in the male's accessory gland. Indeed, all 16 Acp genes tested so far encode seminal proteins detectable only in the male's accessory gland and transferred to the female during mating (Chen et al 1988;Monsma et al 1990;Coleman et al 1995;Bertram et al 1996;Lung et al 2002;Albright 2003;Ravi Ram et al 2005). Additional support that this set of 52 D. melanogaster ACPs truly represents accessory-gland predominant genes stems from the finding that 29 of 46 tested Acp genes showed twofold or higher expression values in germlineless male vs. germlineless female comparisons (6 ACPs were not present on the microarrays) .…”

Section: Methodsmentioning

confidence: 99%

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Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

et al. 2005

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Drosophila melanogaster males transfer seminal fluid proteins along with sperm during mating. Among these proteins, ACPs (Accessory gland proteins) from the male's accessory gland induce behavioral, physiological, and life span reduction in mated females and mediate sperm storage and utilization. A previous evolutionary EST screen in D. simulans identified partial cDNAs for 57 new candidate ACPs. Here we report the annotation and confirmation of the corresponding Acp genes in D. melanogaster. Of 57 new candidate Acp genes previously reported in D. melanogaster, 34 conform to our more stringent criteria for encoding putative male accessory gland extracellular proteins, thus bringing the total number of ACPs identified to 52 (34 plus 18 previously identified). This comprehensive set of Acp genes allows us to dissect the patterns of evolutionary change in a suite of proteins from a single male-specific reproductive tissue. We used sequence-based analysis to examine codon bias, gene duplications, and levels of divergence

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Section: Methodsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

et al. 2005

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“…The predicted molecular weight of Acp29AB (excluding the putative signal peptide) is 24.8 kDa. The higher apparent molecular weight may be due to posttranslational modifications, such as glycosylation; other Acps are known to be glycosylated (Monsma et al 1990;Bertram et al 1996), and sequence data predict that Acp29AB can be N-glycosylated at amino acids 61 and 164 (Wolfner et al 1997). Following mating, Acp29AB is transferred from the male to the female: Acp29AB is absent from the reproductive tracts of virgin females and females 5 min ASM, but is transferred to the female reproductive tract by 7 min ASM ( Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

A Role for Acp29AB, a Predicted Seminal Fluid Lectin, in Female Sperm Storage inDrosophila melanogaster

et al. 2008

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Females of many animal species store sperm for taxon-specific periods of time, ranging from a few hours to years. Female sperm storage has important reproductive and evolutionary consequences, yet relatively little is known of its molecular basis. Here, we report the isolation of a loss-of-function mutation of the Drosophila melanogaster Acp29AB gene, which encodes a seminal fluid protein that is transferred from males to females during mating. Using this mutant, we show that Acp29AB is required for the normal maintenance of sperm in storage. Consistent with this role, Acp29AB localizes to female sperm storage organs following mating, although it does not appear to associate tightly with sperm. Acp29AB is a predicted lectin, suggesting that sugar-protein interactions may be important for D. melanogaster sperm storage, much as they are in many mammals. Previous association studies have found an effect of Acp29AB genotype on a male's sperm competitive ability; our findings suggest that effects on sperm storage may underlie these differences in sperm competition. Moreover, Acp29AB's effects on sperm storage and sperm competition may explain previously documented evidence for positive selection on the Acp29AB locus.

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“…Insemination reactions, which are observed in many Drosophila and other Diptera, occur in the female reproductive tract and result in sperm inactivation. Insemination reactions often result in the formation of a congealed sperm mass that is thought to block egg laying or reinsemination physically (Patterson 1946;Patterson & Stone 1952;Ward & Heed 1970;Asada & Kitagawa 1988;Asada & Fukumitsu 1990;Pitnick et al 1995;Bertram et al 1996;Polak et al 1998; but see also Markow 1997). We saw no evidence of insemination reactions in our crosses, consistent with previous reports in the melanogaster group.…”

Section: Discussionmentioning

confidence: 99%

Gametic incompatibilities between races ofDrosophila melanogaster

Alipaz

Wu²,

Karr

2001

Proc. R. Soc. Lond. B

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Reproductive-isolating mechanisms between nascent species may involve sperm^egg recognition and have been best described in externally fertilizing organisms where such recognition is essential in preventing undesirable fertilizations. However, reproductive barriers in internally fertilizing species di¡er in signi¢cant ways, and a direct role for sperm^egg interactions has yet to be demonstrated. Females of many strains of Drosophila melanogaster from Zimbabwe, Africa, do not mate readily with cosmopolitan males. This polymorphism in mate choice is postulated to represent incipient speciation. We now report that, in one direction, crosses between the above populations produce far fewer o¡spring than reciprocal crosses due to a lower rate of egg hatch. We established that egg inviability in these crosses was due to defects in fertilization. Thus, even in taxa with internal fertilization, gametic incompatibility may be a mechanism relevant to reproductive isolation during incipient speciation.

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Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage

Cited by 118 publications

References 39 publications

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

A Role for Acp29AB, a Predicted Seminal Fluid Lectin, in Female Sperm Storage inDrosophila melanogaster

Gametic incompatibilities between races ofDrosophila melanogaster

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