Localization of telomeres in plant interphase nuclei by in situ hybridization and 3D confocal microscopy

Human telomeres are attached to the nuclear matrix.

“…locates telomeric loci near the surface of mammalian interphase nuclei (van Dekken et al, 1989). These and similar observations in other systems suggest that the ends of eukaryotic chromosomes are located close to the nuclear envelope (Agard and Sedat, 1983;Mathog et al, 1984;Foe and Alberts, 1985;Hochstrasser et al, 1986;Mathog and Sedat, 1989;Chung et al, 1990;Rawlins et al, 1991). This positioning throughout interphase in organisms as diverse as flies, trypanosomes, plants and mammals is likely to be a consequence of specific anchorage of telomeric DNA at the nuclear periphery.…”

“…Rabl (1885) (Sperling and Ludtke, 1981;Cremer et al, 1982;Foe and Alberts, 1985). Furthermore, direct analysis of chromosome topography by three-dimensional microscopy indicates that the ends of interphase chromosomes are close to the nuclear envelope in a wide variety of eukaryotes (Agard and Sedat, 1983;Mathog et al, 1984;Hochstrasser et al, 1986;Mathog and Sedat, 1989;van Dekken et al, 1989;Chung et al, 1990;Rawlins et al, 1991). These cytological data suggest that telomeres could be attached to the nuclear envelope.…”

Chromosomal Localization of 5S rRNA Genes in Vicia faba and Crepis capillaris.

“…1B). The terminal and proximal ends where telomere sequences were located (Rawlins et al 1991) and 18S-28S rRNA genes (Hizume 1992), respectively, were not hybridized with 5S RNA probe. At metaphase heavy signals and weak signals were separated to pairs of dots on sister chromatids of the satellite of M chromosomes (Fig.…”

Comparative Genome Organization in Plants: From Sequence and Markers to Chromatin and Chromosomes

“…Various levels of intranuclear compartmentalization can be regarded: individual chromosomes (Figure 2), euchromatic and heterochromatic regions, the nucleolus ( Figure 5), and regions of active RNA synthesis and processing. Furthermore, telomeres and centromeres may be attached to or closely adjacent to the nuclear envelope (Schwarzacher and Heslop-Harrison, 1990;Rawlins et al, 1991) and occupy defined parts (poles) of the nucleus in many species (Rabl, 1885;Cremer et al, 1982;Anamthawat-Jónsson and Heslop-Harrison, 1990). Cook (1997) has argued that each chromosome in a haploid set has a unique array of transcription units strung along its length and that chromatin fibers will therefore be folded into unique arrays of loops, with homologs sharing similar arrays.…”