Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout

Bailhache, Thierry; Arazam, Aïcha; Klungland, Helge; Aleström, Peter; Breton, Bernard; Jégo, Patrick

doi:10.1002/cne.903470310

Cited by 39 publications

(25 citation statements)

References 34 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA probe which was used for hybridisation (AS1-UpGnRH 236 bp fragment) detected both transgenic antisense and endogenous sGnRH transcripts with a better specificity for sGnRH-antisense (100% homology, 236 bp of complementary sequence) than for rainbow trout sGnRH mRNA (50 bp of complementary sequence with three and one mismatches for sGnRH-I and -II respectively). The antisense RNA was only present in the brain of AS(+) males and females, whereas sGnRH messengers were also present in AS( ) fish, and their size (650-700 bp) corresponded to that reported previously (Bailhache et al 1994, Ashihara et al 1995). An additional smear band of approximately 900 bp was only detected in AS(+) fish and could be the sGnRH-antisense transcript.…”

Section: Pab-directed Expression Of Inserted Genes In Transgenic Troutsupporting

confidence: 66%

Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon

Uzbekova¹,

Chyb²,

Ferrière³

et al. 2000

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS( ) individuals from the same F2 progeny. sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS( ) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described.

show abstract

Section: Pab-directed Expression Of Inserted Genes In Transgenic Troutsupporting

confidence: 66%

Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon

Uzbekova¹,

Chyb²,

Ferrière³

et al. 2000

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…In particular, Parhar et al (1996) could not localize GnRH mRNA by in situ hybridization to preoptic area cells in a cichlid (Oreochromis mossambicus) or salmon (O. nerka) using a probe for 5Trp 7 Leu 8 6GnRH mRNA, yet they readily demonstrated immunoreactivity to GnRH in this region. Similarly, in situ hybridization in masu salmon (Suzuki et al, 1992), rainbow trout, and Atlantic salmon (Bailhache et al, 1994), using 5Trp 7 Leu 8 6GnRH-specific probes, found robust GnRH gene expression in the terminal nerve region yet only weak expression in the preoptic area. Comparing the immunocytochemical staining of GnRH neurons by Amano et al (1991) in masu salmon and Bailhache et al (1994) in Atlantic salmon and rainbow trout with in situ labeling in these species shows significantly less labeling in the preoptic area with in situ probes.…”

Section: Surprising Similarity Between 5trp 7 Leu 8 6gnrh In H Burtomentioning

confidence: 88%

“…Similarly, in situ hybridization in masu salmon (Suzuki et al, 1992), rainbow trout, and Atlantic salmon (Bailhache et al, 1994), using 5Trp 7 Leu 8 6GnRH-specific probes, found robust GnRH gene expression in the terminal nerve region yet only weak expression in the preoptic area. Comparing the immunocytochemical staining of GnRH neurons by Amano et al (1991) in masu salmon and Bailhache et al (1994) in Atlantic salmon and rainbow trout with in situ labeling in these species shows significantly less labeling in the preoptic area with in situ probes. Indeed, Bailhache et al concluded that this could ''.…”

Section: Surprising Similarity Between 5trp 7 Leu 8 6gnrh In H Burtomentioning

confidence: 88%

Genomic Structure and Expression Sites of Three Gonadotropin-Releasing Hormone Genes in One Species

White

Fernald

1998

General and Comparative Endocrinology

View full text Add to dashboard Cite

In the teleost fish, Haplochromis burtoni, gonadotropinreleasing hormone (GnRH) peptide has been localized to three distinct regions in the brain. Each GnRH population is associated with expression of a distinct cDNA as previously described. Here we report the complete genomic sequences encoding these three forms and compare their structural organization, putative regulatory elements, and expression patterns in the body. All three genes share a common structure of four exons: the first exon encodes the 5Ј untranslated region; the second exon encodes the signal sequence, GnRH decapeptide, and the 5Ј end of the GnRH-associated peptide (GAP); the third exon consists entirely of GAP coding sequence; and the fourth exon encodes the 3Ј end of GAP and the 3Ј untranslated region. Each of the three GnRH genes has been shown previously to have a distinct spatial expression pattern in the brain, and here we use reverse transcription and cDNA amplification to demonstrate that each gene is expressed in the body. The gene encoding the releasing form, 5Ser 8 6GnRH, is expressed in the heart, liver, spleen, kidney, and testis, as well as in the preoptic area. The 5His 5 Trp 7 Tyr 8 6GnRH gene is expressed in the testis as well as in the midbrain. The 5Trp 7 Leu 8 6GnRH gene is expressed in the testis and the terminal nerve area. We examined the 500 bp upstream of exon 1 in all three H. burtoni genes and identified putative binding sites for glucocorticoid receptor, androgen receptor, and progesterone receptor, as well as the transcription factors Ap-1 and Sp-1. The genomic sequence encoding the terminal nerve form of GnRH (i.e., 5Trp 7 Leu 8 6GnRH) in H. burtoni is remarkably similar to that encoding the presumed releasing form of GnRH in salmonids, especially in the 3Ј intergenic region. Taken together with phylogenetic and mRNA localization data in salmonids, these data suggest that the gene encoding the releasing form of GnRH in salmonids may not yet be described.

show abstract

“…In situ hybridization was performed according to Salbcrt et al [ 18] and Bailhache et al [19] either on paraffin or cryostat sections. In the case of paraffin sections, the tissue was rinsed first in RNAscfrec phosphate buffer (PB.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Distribution and Expression of Glucocorticoid Receptor mRIMA in the Forebrain of the Rainbow Trout

et al. 1997

Self Cite

View full text Add to dashboard Cite

The expression and distribution of glucocorticoid receptor mRNA was studied in the forebrain of mature female and immature undifferentiated rainbow trout (Oncorhynchus mykiss) by means of Northern blotting and in situ hybridization. A single mRNA species of 7.5 kb was detected in mRNA polyA⁺ prepared from the anterior brain. In situ hybridization was carried out using a ³⁵S-labelled riboprobe corresponding to the A/B-domain (between nucleotides 1224 and 1763) of the recently cloned rainbow trout glucocorticoid receptor cDNA. Comparison of adjacent sections hybridized with the sense and antisense probes allowed detection of a specific signal with a similar distribution pattern in all animals studied. In the telencephalon, a specific hybridization was detected in scattered cells of the dorsal telencephalic hemisphere, but the stronger signal was consistently observed in the dorsal nucleus, and to a lesser degree in the ventral nucleus of the ventral telencephalon. Heavy hybridization staining was consistently observed in all subdivisions of the preoptic nucleus and the nucleus lateralis tuberis, which are the main hypophysiotrophic regions in fish. A weaker signal was detected in the nucleus anterioris periventricularis, nucleus suprachiasmaticus and thalamic region. The presence of a strong signal in virtually all magnocellular neurons of the nucleus preopticus, known for producing vasotocin, isotocin and corticotropin-releasing factor favors a direct function of cortisol in regulating its own secretion.

show abstract

Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout

Cited by 39 publications

References 34 publications

Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon

Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon

Genomic Structure and Expression Sites of Three Gonadotropin-Releasing Hormone Genes in One Species

Distribution and Expression of Glucocorticoid Receptor mRIMA in the Forebrain of the Rainbow Trout

Contact Info

Product

Resources

About