Localization of resistin and its possible roles in the ovary of a vespertilionid bat, Scotophilus heathi

Singh, Ajit; Suragani, Madhuri; Ehtesham, Nasreen Z.; Krishna, Amitabh

doi:10.1016/j.steroids.2014.12.018

Cited by 21 publications

(13 citation statements)

References 31 publications

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“…The first indication that resistin was involved in the regulation of the female reproductive system may be the discovery that resistin was expressed in rat ovaries throughout the oestrous cycle and was elevated in animals with induced ovarian cysts (Jones et al 2009). Subsequent study demonstrated resistin expression in ovarian cells of bovine (Maillard et al 2011), pigs , humans (Niles et al 2012, Reverchon et al 2013b) and the vespertilionid bat (Singh et al 2015). We demonstrated that both gonadotropins and steroid hormones significantly stimulated, while insulin growth factor type 1 (IGF1) and a synthetic agonist of PPARg reduced resistin expression and secretion by cultured porcine ovarian follicles, indicating that ovarian resistin expression can be regulated by several factor (Rak-Mardyła & Drwal 2015.…”

Section: Introductionmentioning

confidence: 75%

“…However, resistin significantly reduced P 4 and E 2 secretion in response to IGF1 in human granulosa cells (Reverchon et al 2013b) and porcine ovarian follicles . In the most recent study, Singh et al (2015) demonstrated also that in vespertilionid bats (Scotophilus heathi), resistin alone significantly stimulated P 4 synthesis, but when acting together with the luteinizing hormone (LH), it also stimulated androgen secretion. A previous study demonstrated that PPARg activation may represent one mechanism of endocrine action of resistin in porcine ovarian follicles (Rak-Mardyła & Drwal 2015).…”

Section: Introductionmentioning

confidence: 99%

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Resistin is a survival factor for porcine ovarian follicular cells

Rak¹,

Drwal²,

Wróbel³

et al. 2015

Reproduction

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Previously, we demonstrated the expression of resistin in the porcine ovary, the regulation of its expression and its direct effect on ovarian steroidogenesis. The objective of this study was to examine the effect of resistin on cell proliferation and apoptosis in a co-culture model of porcine granulosa and theca cells. First, we analysed the effect of resistin at 1 and 10 ng/ml alone or in combination with FSHand IGF1 on ovarian cell proliferation with an alamarBlue assay and protein expression of cyclins A and B using western blot. Next, the mRNA and protein expression of selected pro-apoptotic and pro-survival regulators of cell apoptosis, caspase-9, -8 and -3 activity and DNA fragmentation using real time PCR, western blot, fluorescent assay and an ELISA kit, respectively, were analysed after resistin treatment. Furthermore, we determined the effect of resistin on the protein expression of ERK1/2, Stat and Akt kinase. Using specific inhibitors of these kinases, we also checked caspase-3 activity and protein expression. We found that resistin, at both doses, has no effect on cell proliferation. The results showed that resistin decreased pro-apoptotic genes, which was confirmed on protein expression of selected factors. We demonstrate an inhibitory effect of resistin on caspase activity and DNA fragmentation. Finally, resistin stimulated phosphorylation of the ERK1/2, Stat and Akt and kinases inhibitors reversed resistin action on caspase-3 activity and protein expression to control. All of these results showed that resistin has an inhibitory effect on porcine ovarian cell apoptosis by activation of the MAPK/ERK, JAK/Stat and Akt/PI3 kinase signalling pathways. Reproduction (2015) 150 343-355

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Resistin is a survival factor for porcine ovarian follicular cells

Rak¹,

Drwal²,

Wróbel³

et al. 2015

Reproduction

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“…Similarly, studies on human granulosa cells treated with resistin have also shown reduced steroid hormone secretion in response to IGF1 (Reverchon et al 2013). However, in human theca cells in normal cycling premenopausal women (Munir et al 2005), pig ovary (Rak-Mardyła et al 2013 and bats (Singh et al 2015), a stimulatory effect of resistin at physiological doses on androgen production was observed. Conversely, in porcine ovarian follicles, resistin decreased gonadotropinand IGF1-induced steroid hormone secretion by inhibiting the protein expressions of 3βHSD, 17βHSD and CYP19A1 (Rak et al 2015a).…”

Section: Adiponectin and Resistin In Vitro Effects On Ovarian Steroidmentioning

confidence: 94%

“…↑, increase, ↓ decrease. (a) Richards et al 2012, (b) Maillard et al 2010, (c) Chabrolle et al 2007Chabrolle et al , 2009 Maleszka et al 2014, (e) Anuradha et al 2014, (f) Lagaly et al 2008, (g) Spicer et al 2011, (h) Reverchon et al 2013, (i) Rak et al 2015a, (j) Maillard et al 2011, (k) Munir et al 2005, (l) Rak-Mardyła et al 2013 Singh et al 2015.…”

Section: Adiponectin Adipors and Resistin Expression In The Ovarymentioning

confidence: 99%

Adiponectin and resistin: potential metabolic signals affecting hypothalamo-pituitary gonadal axis in females and males of different species

Rak¹,

Mellouk²,

Froment³

et al. 2017

Reproduction

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Adipokines, including adiponectin and resistin, are cytokines produced mainly by the adipose tissue. They play a significant role in metabolic functions that regulate the insulin sensitivity and inflammation. Alterations in adiponectin and resistin plasma levels, or their expression in metabolic and gonadal tissues, are observed in some metabolic pathologies, such as obesity. Several studies have shown that these two hormones and the receptors for adiponectin, AdipoR1 and AdipoR2 are present in various reproductive tissues in both sexes of different species. Thus, these adipokines could be metabolic signals that partially explain infertility related to obesity, such as polycystic ovary syndrome (PCOS). Species and gender differences in plasma levels, tissue or cell distribution and hormonal regulation have been reported for resistin and adiponectin. Furthermore, until now, it has been unclear whether adiponectin and resistin act directly or indirectly on the hypothalamo-pituitary-gonadal axis. The objective of this review was to summarise the latest findings and particularly the species and gender differences of adiponectin and resistin on female and male reproduction known to date, based on the hypothalamo-pituitary-gonadal axis.Reproduction (2017) 153 R215-R226

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“…However, approaches on the reproductive biology of females are limited and their reproductive pattern are defined based only on investigations of the external morphological characteristics of their body (dilated abdomen, visible nipples with secretion and the absence of hair around it) (Racey 1988, Gannon & Willig 1992, Esbérard 2002. In other countries, there is some recent research on female gonadal histophysiology focusing on folliculogenesis and ovulation of different species of bats located in Colombia (Komar et al 2007), China (Wang et al 2008), Canada (Rintoul & Brigham 2014), Mexico (Antonio-Rubio Porras-Gómez & Moreno-Mendoza 2013) and India (Sastry & Pillai 2013, Singh et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the reproductive stage and ovarian histomorphometry of Dermanura cinerea (Chiroptera: Phyllostomidae) in an Atlantic Forest fragment of Pernambuco, northeastern Brazil

et al. 2018

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This study aimed to analyze the reproductive stage, histology and morphometry of the ovary of Dermanura cinerea in an Atlantic Forest fragments in the Biological Reserve of Saltinho, Pernambuco, Brazil. Adult females were captured monthly by mist net, during two consecutive nights from June/2014 to November/2015. The meteorological data were provided by the National Institute of Meteorology and grouped together with the reproductive data, in six periods: period I (June to August/2014), period II (September to November/2014), period III (December/2014 to February/2015), period IV (March to May/2015), period V (June to August/2015) and period VI (September to November/2015). The females were classified according to their reproductive stages in: Inactive, pregnant, lactating and postlactating. Among the 25 females caught, 10 inactive females were selected for the histomorphometric analysis of the ovaries and then they were euthanized. After that, the specimens had their ovaries removed, fixed, processed and included in Paraplast. The organs were cut and stained by Hematoxylin - Eosin and the histological slides produced were submitted to the appropriate optical microscope analysis. Each type of ovarian follicle was estimated according to the histological characteristics evidenced for other mammals. In the morphometric analysis, it was considered: The area of the ovary (measured in square millimeter), Quantification of different types of ovarian follicles (unilaminar and multilaminar primary follicles, antral and matures) and corpus luteum, as well as the area of each by those different follicles (measured in square micrometer) The results indicated that 16 females were inactive, 5 were lactating, 4 were pregnant and no post-lactating. The histological analysis revealed that the ovaries of D. cinerea exhibit characteristics, structures and cellular types similar to other mammals. The morphometric data of the study area were statistically evaluated using the Student’s t-test. Therefore, ovarian histomorphometric analysis are very important to infer with greater certainty about the reproduction of a species. Thus, it was observed that females of D. cinerea exhibit two reproductive peaks, that is, they can give birth either at the end of dry periods or from the beginning to the middle of the rainy season. The main feature observed in the ovary of this species, found in the Atlantic Forest area of the state of Pernambuco, is the preparation of the ovarian cortex to ovulate in months of high rainfall throughout the year. Therefore, this species tries to adjust its reproductive period with the variations of the environmental conditions, so that the reproduction might occur during the period more favorable for the maintenance of their offspring.

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Localization of resistin and its possible roles in the ovary of a vespertilionid bat, Scotophilus heathi

Cited by 21 publications

References 31 publications

Resistin is a survival factor for porcine ovarian follicular cells

Resistin is a survival factor for porcine ovarian follicular cells

Adiponectin and resistin: potential metabolic signals affecting hypothalamo-pituitary gonadal axis in females and males of different species

Analysis of the reproductive stage and ovarian histomorphometry of Dermanura cinerea (Chiroptera: Phyllostomidae) in an Atlantic Forest fragment of Pernambuco, northeastern Brazil

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