Localization of replication origins in pea chloroplast DNA.

Meeker, R.; Nielsen, Brent L.; Tewari, Κ. Κ.

doi:10.1128/mcb.8.3.1216

Cited by 52 publications

(44 citation statements)

References 21 publications

(23 reference statements)

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“…This observation is surprising in the light of the fact that the in vivo mechanism [20] of ctDNA replication requires two origins to produce replicating molecules with theta structures. This discrepancy may be explained by an anomalous behaviour of the pea OriA sequence in the heterologous tobacco system.…”

Section: Discussionmentioning

confidence: 55%

“…with the replicative system, observed DNA synthesis as measured by the tritium incorporation assay [20] was 5 -6-fold less compared to template molecules bearing the Ori sequences. Analysis of the denatured nascent products revealed that the template-sized strands were few in number while the majority of the products was of small molecular size.…”

Section: Kinetics Of Dna Replicationmentioning

confidence: 99%

“…Reactions were carried out essentially as described by Meeker et al [20]. Replicative products were analysed using denaturing or non-denaturing agarose gel electrophoresis.…”

Section: Replication In Vitromentioning

confidence: 99%

“…The soluble replicative system from pea chloroplasts was prepared by the protocol of Meeker et al [20] with minor modifications. Intact pea chloroplasts were isolated and disrupted by Triton X-100 [17].…”

Section: Replicative Systemmentioning

confidence: 99%

“…This small Cairns' structure then proceeds bi-directionally until termination takes place. Meeker et al [20] have mapped the two replication origins or D-loops by electron…”

mentioning

confidence: 99%

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Characterisation and mode of in vitro replication of pea chloroplast OriA sequences

Reddy

Choudhury

Kumar

et al. 1994

European Journal of Biochemistry

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A partially purified replicative system of pea chloroplast that replicates recombinant DNAs containing pea chloroplast origin sequences has been characterised. Polymerisation by this system is very fast and insensitive to chain terminators like dideoxynucleotides, arabinosylcytosine 5'-triphosphate, etc. Both strands of template DNA are synthesized and single-stranded DNA templates undergo more than one round of replication.When sequences of either of the two chloroplast origins of replication (OriA or OriB) are used as templates, the replicative intermediates are found to have sigma structures. Electron microscopic analysis of the sigma structures restricted with various enzymes reveals that the initiation site of in vitro replication maps near the displacement-loop regions where replication initiates also in vivo. Although the observed replication initiation in the OriA recombinant template is chloroplast-DNAspecific, the mode of replication is different from that observed in vivo with intact ctDNA. However, when the template DNA contains both the OriA and OriB sequences, the in vitro replication proceeds in the theta mode, the mode of replication usually observed in vivo.The complex process of DNA replication has been defined for only a few biological systems such as plasmids, bacteriophages, bacteria and viruses [l-61. So far, little is known about the DNA replication processes in plant systems except for few reports in the field of plant organelles [7-111. Chloroplast DNA (ctDNA) of higher plants seems to be an attractive system for investigation of the molecular details of replication. The chloroplast genomic size of about 150 kb is large but not too large for recombinant manipulations and the genome exists in multiple copies. Understanding the molecular biology of ctDNA replication would not only reveal the detailed characteristics of this system [12-141 but also help solve problems related to stable transformation of the chloroplast organelle. Thus, development of an in vitro replication system derived from higher plant chloroplasts is important both in helping to define DNA metabolism in general as well as advancing the technology of transgenic plant production [15-181. Replicative intermediates of ctDNA from pea leaves have been well characterised using electron microscopic techniques [19]. In pea and maize ctDNA, it was shown that replication begins by forming two displacement loops (Dloops) which expand towards each other to form a theta structure. This small Cairns' structure then proceeds bi-directionally until termination takes place. Meeker et al. [20] have mapped the two replication origins or D-loops by electron Correspondence to S . K.

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Section: Discussionmentioning

confidence: 55%

Section: Kinetics Of Dna Replicationmentioning

confidence: 99%

“…Reactions were carried out essentially as described by Meeker et al [20]. Replicative products were analysed using denaturing or non-denaturing agarose gel electrophoresis.…”

Section: Replication In Vitromentioning

confidence: 99%

Section: Replicative Systemmentioning

confidence: 99%

“…This small Cairns' structure then proceeds bi-directionally until termination takes place. Meeker et al [20] have mapped the two replication origins or D-loops by electron…”

mentioning

confidence: 99%

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Characterisation and mode of in vitro replication of pea chloroplast OriA sequences

Reddy

Choudhury

Kumar

et al. 1994

European Journal of Biochemistry

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Plastid Genome Stability and Repair

Zampini

Truche

Lepage

et al. 2017

Somatic Genome Variation in Animals, Plants, and Microorganisms

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Detection and characterization of a plastid envelope DNA-binding protein which may anchor plastid nucleoids.

et al. 1993

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Chloroplast DNA (cpDNA) binds to the envelope membrane of actively dividing chloroplasts (plastids) in young pea leaves. South‐western blotting was used to identify and characterize the protein involved in the binding of cpDNA to the envelope membrane. A 130 kDa protein in the inner chloroplast (plastid) envelope membrane binds specific sequences within the cpDNA. These included a 0.41 kbp sequence located upstream of the psaAB gene, a 0.57 kbp sequence located downstream of the petA gene and a 1.2 kbp sequence located within the rpoC2 gene. The protein was detected in the envelope membrane of young pea leaves in which the cpDNA had been located by fluorescence microscopy at the chloroplast periphery, whereas it was undetectable in mature leaves. We therefore propose that the 130 kDa protein is involved in the binding of cpDNA to the envelope membrane, and named it plastid envelope DNA‐binding protein.

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Localization of replication origins in pea chloroplast DNA.

Cited by 52 publications

References 21 publications

Characterisation and mode of in vitro replication of pea chloroplast OriA sequences

Characterisation and mode of in vitro replication of pea chloroplast OriA sequences

Plastid Genome Stability and Repair

Detection and characterization of a plastid envelope DNA-binding protein which may anchor plastid nucleoids.

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