Localization of Rat Liver α-Foetoprotein by Cell Affinity Labelling with Tritiated Oestrogens

Tissular Immunoenzymatic Detection of Hepatic Alphafetoprotein in Human Hepatomas

“…The a-FP-secreting cell population represents a minor cell population as compared to tire whole tumoral tissue; on the other hand, the a-FP-secreting cells are not grouped in secreting focuses. These observations confirm previous results obtained in hepatomas with different histological a-FP-detection techniques (7,19,21,45,46). On the contrary, it has not been possible to discover any preferential localization of the a-FPsecreting cells inside the tumoral tissue as it was suggested by Uriel et al (46).…”

“…Furthermore, the demonstration of a-FP secretion in human and experimental hepatomas has been achieved using various histological methods: immuno fluorescence (7,19,21,41), autoradiography (45)(46)(47) and immunoenzymatic labelling using peroxidase (33,34). All these methods show the cytoplasmic localization of a-FP in tumoral cells, but, in human hepatomas, the fine a-FP cellular localization is still unknown.…”

Localization of T and B cells and alpha fetoprotein in hepatic biopsies from patients with liver disease.

“…Our findings appear to be the first clear documentation of cellular localization of AFP in the liver of patients with alcoholic hepatitis. However, cell localization of AFP does not constitute definitive evidence that such cells synthesize this material as stressed by previous workers using affinity labeling of cellular AFP with tritiated estrogens (47).…”

Alpha1-Fetoprotein Biosynthesis During the Growth Cycle of Differentiated Fetal Rat Hepatocytes in Primary Monolayer Culture

“…Because pancreatic, pituitary, adrenocortical, and thyroid hormones are implicated,' it seemed pertinent to explore the capac- ity of these cultured cells to synthesize a,F (I, 10), an oncoprotein (1, 2) that has been reported to have a high binding affinity for estrogens (30,31) . The initial observations described in this report suggest that the appearance of synthesized a,F is related to the position of cultured fetal hepatocytes in both the growth and the cell cycle .…”