Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling

Guzmán, Paula; Fernández, Victoria; García, María L.; Khayet, M.; Fernández, Agustín; Gil, Luis

doi:10.1016/j.plaphy.2013.12.023

Cited by 78 publications

(82 citation statements)

References 32 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cuticular waxes (soluble cuticular lipids) are complex mixtures of aliphatic (mainly long-chain fatty acids, alcohols, alkanes, aldehydes, esters and ketones) and aromatic compounds (Jetter et al, 2006). Polysaccharides present in the cuticle are similar to those found in the cell wall (i.e., cellulose, hemicelluloses and pectins; López-Casado et al, 2007; Guzmán et al, 2014b). Based on the relative abundance of chemical constituents (Schmidt and Schönherr, 1982; Heredia, 2003; Samuels et al, 2008), the cuticle has been conceived as a hydrophobic membrane.…”

Section: Introductionmentioning

confidence: 92%

“…It has been traditionally assumed that cutin/cutan and waxes are found both in the CP and CL, while the presence of polysaccharides is restricted to the CL (von Mohl, 1847; Jeffree, 2006). By contrast, the presence of cellulose and pectins in both cuticular layers has been recently reported (Guzmán et al, 2014b). For various species and organs, different patterns of cuticular ultra-structure have been categorized on the basis of transmission electron microscopy (TEM) observations (Holloway, 1982; Jeffree, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Guzmán

Fernández

Graça³

et al. 2014

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.

show abstract

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Guzmán

Fernández

Graça³

et al. 2014

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Isolation of the polysaccharide fraction of mature tomato fruit cuticles showed an equimolecular contribution of the three main components of cell wall: cellulose, hemicellulose, and pectin (López-Casado et al, 2007). Recently, using enzyme-gold labeling, Guzmán et al (2014) reported the identification and location of cellulose and pectin in isolated cuticles of Eucalyptus L'Hér., Populus L., and Pyrus L. leaves. In this work, results obtained by the use of antibodies against the ordered non-esterified pectin, the gelatinous esterified pectin, and crystalline cellulose allowed us to draw the following molecular scenario.…”

Section: The Discontinuous Construction Of the Cutinized Cell Wallmentioning

confidence: 99%

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

2015

View full text Add to dashboard Cite

The epidermis plays a pivotal role in plant development and interaction with the environment. However, it is still poorly understood, especially its outer epidermal wall: a singular wall covered by a cuticle. Changes in the cuticle and cell wall structures are important to fully understand their functions. In this work, an ultrastructure and immunocytochemical approach was taken to identify changes in the cuticle and the main components of the epidermal cell wall during tomato fruit development. A thin and uniform procuticle was already present before fruit set. During cell division, the inner side of the procuticle showed a globular structure with vesicle-like particles in the cell wall close to the cuticle. Transition between cell division and elongation was accompanied by a dramatic increase in cuticle thickness, which represented more than half of the outer epidermal wall, and the lamellate arrangement of the non-cutinized cell wall. Changes in this non-cutinized outer wall during development showed specific features not shared with other cell walls. The coordinated nature of the changes observed in the cuticle and the epidermal cell wall indicate a deep interaction between these two supramolecular structures. Hence, the cuticle should be interpreted within the context of the outer epidermal wall.

show abstract

“…In the case of holm oak adaxial leaf surfaces, trichomes and the remaining scars after trichome shedding may play a key role in water absorption. The high surface tension, polarity, and H-bonding capacity of water theoretically pose restrictions for the penetration of this liquid through the cuticle (Guzmán et al, 2014(Guzmán et al, a, 2014b and also via stomata (Schönherr and Bukovac, 1972;Burkhardt et al, 2012). The actual contribution of leaf trichomes to the absorption of water and solutes remains unclear (Fernández et al, 2014).…”

Section: Leaf Water Uptake and Ecophysiological Implicationsmentioning

confidence: 99%

Wettability, Polarity, and Water Absorption of Holm Oak Leaves: Effect of Leaf Side and Age

et al. 2014

Self Cite

View full text Add to dashboard Cite

Plant trichomes play important protective functions and may have a major influence on leaf surface wettability. With the aim of gaining insight into trichome structure, composition, and function in relation to water-plant surface interactions, we analyzed the adaxial and abaxial leaf surface of holm oak (Quercus ilex) as a model. By measuring the leaf water potential 24 h after the deposition of water drops onto abaxial and adaxial surfaces, evidence for water penetration through the upper leaf side was gained in young and mature leaves. The structure and chemical composition of the abaxial (always present) and adaxial (occurring only in young leaves) trichomes were analyzed by various microscopic and analytical procedures. The adaxial surfaces were wettable and had a high degree of water drop adhesion in contrast to the highly unwettable and water-repellent abaxial holm oak leaf sides. The surface free energy and solubility parameter decreased with leaf age, with higher values determined for the adaxial sides. All holm oak leaf trichomes were covered with a cuticle. The abaxial trichomes were composed of 8% soluble waxes, 49% cutin, and 43% polysaccharides. For the adaxial side, it is concluded that trichomes and the scars after trichome shedding contribute to water uptake, while the abaxial leaf side is highly hydrophobic due to its high degree of pubescence and different trichome structure, composition, and density. Results are interpreted in terms of water-plant surface interactions, plant surface physical chemistry, and plant ecophysiology.

show abstract

Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling

Cited by 78 publications

References 32 publications

Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

Wettability, Polarity, and Water Absorption of Holm Oak Leaves: Effect of Leaf Side and Age

Contact Info

Product

Resources

About