Localization of Paramyosin, Myosin, and a Heat Shock Protein 70 in Larval and Adult Brugia malayi

Abstract

Recombinant filarial proteins are of interest as potentially protective immunogens for lymphatic filariasis. We have previously identified paramyosin, myosin, and a heat shock protein 70 (HSP) 70 as prominent immunogens in individuals residing in an area endemic for lymphatic filariasis. Our goal in the present work was to identify the Brugia malayi tissues that contain these proteins. Polyclonal rabbit antisera with high levels of immunoglobulins to each of these proteins were prepared for use in indirect immunofluorescence microscopy studies of third-and fourth-stage larvae (L3's and L4's) and adult worms. Myosin and paramyosin were found within the longitudinal somatic musculature in all of these life stages. In L4's and adult worms, myosin and paramyosin were also detected within the walls of the reproductive and alimentary tracts of male and female worms. HSP 70 was evident within the somatic musculature, hypodermis, lateral chords, alimentary tract, and reproductive structures in L4's and adult worms. HSP 70 was not detected in sections of freshly obtained L3's. However, L3's cultured at 37 C for 24 hr before fixation demonstrated a classic heat shock response. In these larvae, intracellular HSP 70 was observed in all tissues. None of the antigens studied appeared to be located on cuticular surfaces.

Molecular cloning and characterization ofEchinostoma caproniheat shock protein-70 and differential expression in the parasite derived from low- and high-compatible hosts

“…These findings seem similar to those for HSP70 of Brugia malayi, being present in the somatic musculature, hypodermis and reproductive structures (Schmitz et al 1996) and also support the recent finding that HSP70 is one of the most abundant proteins in S. mansoni egg secretome (Cass et al 2007). These findings seem similar to those for HSP70 of Brugia malayi, being present in the somatic musculature, hypodermis and reproductive structures (Schmitz et al 1996) and also support the recent finding that HSP70 is one of the most abundant proteins in S. mansoni egg secretome (Cass et al 2007).…”

“…A study of Brugia malayi has detected HSP70 in musculature, hypodermis and reproductive organs (Schmitz et al 1996), and a recent report described it as one of the most abundant surface antigens in Schistosoma mansoni egg secretions based on proteomic analysis (Cass et al 2007). A study of Brugia malayi has detected HSP70 in musculature, hypodermis and reproductive organs (Schmitz et al 1996), and a recent report described it as one of the most abundant surface antigens in Schistosoma mansoni egg secretions based on proteomic analysis (Cass et al 2007).…”

Vaccination of calves with yeast‐ and bacterial‐expressed paramyosin from the bovine lungworm Dictyocaulus viviparus

“…Paramyosin (PMY), commonly known as a muscle protein of invertebrates including D. viviparus , has been considered as a promising vaccine candidate as it is transcribed throughout all developmental stages of bovine lungworms . PMY of D. viviparus displays immunomodulatory functions by binding to collagen and IgG , similar to those described for Taenia crassiceps ‐PMY .…”

The ImmunodominantBrugia malayiParamyosin as a Marker of Current Infection withWuchereria bancroftiAdult Worms

“…Paramyosin has been characterized as a myofibrillar protein found only in invertebrates. In B. malayi, it is located within the longitudinal somatic musculature of L 3 , L 4 , and adult worms (21). Here we show that it is expressed in Mf as well.…”