Localization of oxytocin binding sites in the human brainstem and upper spinal cord: an autoradiographic study

Loup, Fabienne; Tribollet, Eliane; Dubois‐Dauphin, Michel; Pizzolato, Gilberto; Dreifuss, J. J.

doi:10.1016/0006-8993(89)90317-x

Cited by 109 publications

(79 citation statements)

References 20 publications

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“…So far, only 1 group has unsuccessfully attempted to localize OXT receptors in the amygdala, using a relatively nonspecific approach (autoradiography). 9,48 More studies are necessary to clearly show or exclude OXT receptor presence and abundance in the human brain. However, magnocellular OXT neurons project to areas such as the central amygdala, 90 which may indicate 1 way in which OXT influences the amygdala.…”

Section: Discussionmentioning

confidence: 99%

“…The reward system influences social behaviour by driving the evaluation and interpretation of choices and their associated values. 47 It also houses a large proportion of the OXT receptors in the human brain, the highest concentration of which is located in the substantia nigra (SN), 9,48 an area heavily implicated in reward processing, prediction error and dopamine (DA) signalling. 5,9,48 However, this area is very small, and its between-subjects variability makes it difficult to show robust activation in human imaging studies.…”

Section: Fmri Studies In Humansmentioning

confidence: 99%

“…5,76 In humans, the role of the amygdala in social cognition 77 and, notably, facial processing 65 has established it as a very important region in OXT research. Although no OXT receptors have been detected in the amygdala in any human studies 5,9,48 its size in humans has been shown to vary in relation to the OXT gene 78,79 and it is consistently implicated in studies involving OXT administration (Table 3).…”

Section: Amygdalamentioning

confidence: 99%

See 2 more Smart Citations

Neurophysiological effects of acute oxytocin administration: systematic review and meta-analysis of placebo-controlled imaging studies

Wigton

Raduà

Allen

et al. 2015

jpn

167

120

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Section: Discussionmentioning

confidence: 99%

Section: Fmri Studies In Humansmentioning

confidence: 99%

Section: Amygdalamentioning

confidence: 99%

See 1 more Smart Citation

Neurophysiological effects of acute oxytocin administration: systematic review and meta-analysis of placebo-controlled imaging studies

Wigton

Raduà

Allen

et al. 2015

jpn

167

120

View full text Add to dashboard Cite

“…As holds true for nesfatin-1-induced effects on food intake, it has been shown that the hypothalamic nesfatin-1-mediated effects on cardiovascular function are abolished by pharmacological blockade of central melanocortin and oxytocin receptors (28,29). Since both melanocortin and oxytocin receptors are expressed on neurons in the NTS (2,12,15), it is tempting to speculate that oxytocin and melanocortin responsive neurons, and potentially others as well, are acting together at the level of NTS to exert effects that are ultimately integrated to produce a tachycardic and vasopressor response. Future experiments will thus be aimed at evaluating the contribution of the melanocortin and oxytocin systems to nesfatin-1-induced cardiovascular effects in the medulla.…”

Section: Discussionmentioning

confidence: 99%

Nesfatin-1 influences the excitability of neurons in the nucleus of the solitary tract and regulates cardiovascular function

Mimee

Smith

Ferguson

2012

American Journal of Physiology-Regulatory, Integrative and Comp

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Nesfatin-1 has been identified as one of the most potent centrally acting anorexigenic peptides, and it has also been shown to play important roles in the control of cardiovascular function. In situ hybridization and immunohistochemical studies have revealed the expression of nesfatin-1 throughout the brain and, in particular, in the medullary autonomic gateway known as the nucleus of the solitary tract (NTS). The present study was thus undertaken to explore the cellular correlates and functional roles of nesfatin-1 actions in the medial NTS (mNTS). Using current-clamp electrophysiology recordings from mNTS neurons in slice preparation, we show that bathapplied nesfatin-1 directly influences the excitability of the majority of mNTS neurons by eliciting either depolarizing (42%, mean: 7.8 Ϯ 0.8 mV) or hyperpolarizing (21%, mean: Ϫ8. 2 Ϯ 1.0 mV) responses. These responses were observed in all electrophysiologically defined cell types in the NTS and were site specific and concentration dependent. Furthermore, post hoc single cell reverse transcriptase polymerase reaction revealed a depolarizing action of nesfatin-1 on NPY and nucleobindin-2-expressing mNTS neurons. We have also correlated these actions of nesfatin-1 on neuronal membrane potential with physiological outcomes, using in vivo microinjection techniques to demonstrate that nesfatin-1 microinjected into the mNTS induces significant increases in both blood pressure (mean AUC ϭ 3354.1 Ϯ 750.7 mmHg·s, n ϭ 6) and heart rate (mean AUC ϭ 164.8 Ϯ 78.5 beats, n ϭ 6) in rats. Our results provide critical insight into the circuitry and physiology involved in the profound effects of nesfatin-1 and highlight the NTS as a key structure mediating these autonomic actions. microinjection; platch clamp; peptides NESFATIN-1 has been identified as one of the most potent centrally acting anorexigenic peptides (17). An 82 amino acid cleavage product of the nucleobindin-2 (NUCB2) protein, nesfatin-1 acutely and chronically reduces feeding in rodents when injected either intracerebroventricularly or peripherally, and these effects are dependent on melanocortin and oxytocin signaling (16,17,22,29). Furthermore, treatment with nesfatin-1 neutralizing antibodies markedly increases food consumption and weight gain in animals, underscoring the critical physiological, tonic inhibitory control this peptide exerts over ingestive behaviors (17). In addition to its remarkable effects on food intake, nesfatin-1 has also been shown to play important roles in the control of cardiovascular function, water intake, blood glucose regulation, stress responses, and puberty onset (6,8,23,28).In line with its numerous functions, comprehensive immunohistochemical studies have revealed a widespread distribution of nesfatin-1 throughout the brain. Notably, the nesfatin-1 peptide is strongly expressed in regions involved in the control of energy homeostasis and autonomic function in both hypothalamic and medullary centres (5), including the autonomic gateway known as the nucleus of the solitary tract (...

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“…Oxytocin-responsive cells and OT-receptors are found along the neural axis (13,16,38,40,47). Cell signaling generated by OT is mediated via OT receptors, which are typical members of the rhodopsin-type (class I) G protein-coupled receptor (GPCR) family (5).…”

Section: Physiological Studiesmentioning

confidence: 99%

Stimulation of the hypothalamic paraventricular nucleus modulates cardiorespiratory responses via oxytocinergic innervation of neurons in pre-Bötzinger complex

Mack¹,

Wu²,

Kc³

et al. 2007

Journal of Applied Physiology

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Previously we reported that oxytocin (OT)-containing neurons of the hypothalamic paraventricular nucleus (PVN) project to the preBötzinger complex (preBötC) region and phrenic motoneurons innervating the diaphragm (D). The aim of these studies was to determine pathways involved in PVN stimulation-induced changes in upper airway and chest wall pumping muscle activity. In addition, we determined the role of OT-containing neurons in the PVN in mediating increased respiratory output elicited by PVN stimulation. Neuroanatomical experiments, using pseudorabies virus (PRV) as a transneuronal tracer in C8 spinalectomized animals showed that PVN neurons project to hypoglossal motoneurons innervating the genioglossus (GG) muscle. Furthermore, microinjection of the PVN with bicuculline, a GABA A receptor antagonist, significantly increased (P<0.05) peak electromyographic activity of GG (GG EMG ) and of D EMG , frequency discharge, and arterial blood pressure (BP) and heart rate. Prior injection of oxytocin antagonist [d-(CH2)5, Tyr(Me)2,Orn8]-vasotocin(OVT) intracisternally or blockade of oxytocin receptors in the preBötC region with oxytocin antagonist L-368,899, diminished GG EMG and D EMG responses and blunted the increase in BP and heart rate to PVN stimulation. These data show that PVN stimulation affects central regulatory mechanisms via the preBötC region controlling both respiratory and cardiovascular functions. The parallel changes induced by PVN stimulation were mediated mainly through an OT-OT receptor signaling pathway.

show abstract

Localization of oxytocin binding sites in the human brainstem and upper spinal cord: an autoradiographic study

Cited by 109 publications

References 20 publications

Neurophysiological effects of acute oxytocin administration: systematic review and meta-analysis of placebo-controlled imaging studies

Neurophysiological effects of acute oxytocin administration: systematic review and meta-analysis of placebo-controlled imaging studies

Nesfatin-1 influences the excitability of neurons in the nucleus of the solitary tract and regulates cardiovascular function

Stimulation of the hypothalamic paraventricular nucleus modulates cardiorespiratory responses via oxytocinergic innervation of neurons in pre-Bötzinger complex

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