Localization of Nuclear Functions as Revealed by Ultrastructural Autoradiography and Cytochemistry

Bouteille, M.; Laval, Monique; Dupuy-Coin, A.M.

doi:10.1016/b978-0-12-147601-4.50009-9

Cited by 87 publications

(60 citation statements)

References 188 publications

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“…The diameter of these spherical structures varies between 0.1 and 1 ]lm. Further nuclear bodies have been described and classified according to their morphology (Bouteille et al 1974). In addition, electron microscopy studies revealed nuclear particles, which occur in clusters and mayaiso be part of a loose fibre network (Monneron & Bernhard 1969).…”

Section: Introductionmentioning

confidence: 99%

Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries

et al. 1993

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The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bodies, revealed distinctly different distribution patterns in these cell types. Heat shock experiments confirmed that the distribution patterns also depend on physiological parameters. Using a combination of fluorescence in situ hybridization and immunodetection protocols, individual chromosome domains were visualized simultaneously with the Sm antigen or the transcript of an integrated human papilloma virus genome. Three-dimensional analysis of fluorescence-stained target regions was performed by confocallaser scanning microscopy. RNA transcripts and components of the splicing machinery were found to be generally excluded from the interior of the territories occupied by the individual chromosomes. Based on these findings we present a model for the functional compartmentalization of the cell nucleus. According to this model the space between chromosome domains, including the surface areas of these domains, defines a three-dimensional networklike compartment, termed the interchromosome domain (ICO) compartment, in wh ich transcription and splicing of mRNA occurs.

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Section: Introductionmentioning

confidence: 99%

Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries

et al. 1993

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“…Since the pioneering electron microscopic studies of Bernhard and coworkers, it has became clear that the nucleus of higher eukaryotic cells is a very complex compartment organized into distinct structural domains (Monneron and Bernhard, 1969;Bouteille et al, 1974). Early work from these groups has established clearly that RNA/protein complexes in the nucleus are localized to distinct types of structures that include the nucleolus, the perichromatin fibrils and perichromatin granules, the clusters of interchromatin granules, and the coiled body (reviewed in Monneron and Bernhard, 1969;Smetana and Busch, 1974).…”

Section: Introductionmentioning

confidence: 99%

The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.

Rebelo

Almeida

Ramos

et al. 1996

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The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The results indicate that adenovirus infection induces the disassembly of coiled bodies and that this effect is probably secondary to the block of host protein synthesis induced by the virus. Furthermore, coiled bodies are shown to be very labile structures, with a half-life of -2 h after treatment of HeLa cells with protein synthesis inhibitors. After blocking of protein synthesis, p80 coilin was detected in numerous microfoci that do not concentrate snRNP. These structures may represent precursor forms of the coiled body, which goes through a rapid cycle of assembly/disassembly in the nucleus and requires ongoing protein synthesis to reassemble.

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“…These are electrondense, often spherical structures in nuclei [Bouteille et al, 1974;de The et al, 19601. PML bodies have a diameter of 0.3 to 1.0 km and are tightly associated to the nuclear matrix [Stuurman et al, 19921.…”

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confidence: 99%

“…PML bodies belong to a morphologically defined class of nuclear structures: the nuclear bodies [for reviews see Bouteille et al, 1974;Brasch and Ochs, 19921. These are electrondense, often spherical structures in nuclei [Bouteille et al, 1974;de The et al, 19601.…”

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PML-containing nuclear bodies: Their spatial distribution in relation to other nuclear components

et al. 1996

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PML-containing nuclear bodies: their spatial distribution in relation to other nuclear components Grande, M.A.; van der Kraan, K.; van Steensel, B.; Schul, W.; de The, H.; van der Voort, H.T.M.; de Jong, L.; van Driel, R. Link to publicationCitation for published version (APA): Grande, M. A., van der Kraan, K., van Steensel, B., Schul, W., de The, H., van der Voort, H. T. M., ... van Driel, R. (1996). PML-containing nuclear bodies: their spatial distribution in relation to other nuclear components. Journal of Cellular Biochemistry, 63, 280-291. 3.0.CO;2-T" class="link">https://doi.org/10.1002/(SICI)1097-4644(19961201)63:33.0.CO;2-T General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. The PML protein is a human growth suppressor concentrated in 10 to 20 nuclear bodies per nucleus (PML bodies). Disruption of the PML gene has been shown to be related to acute promyelocytic leukaemia (APL). To obtain information about the function of PML bodies we have investigated the 3D-distribution of PML bodies in the nucleus of T24 cells and compared it with the spatial distribution of a variety of other nuclear components, using fluorescence dual-labeling immunocytochemistry and confocal microscopy. Results show that PML bodies are not enriched in nascent RNA, the splicing component U2-snRNP, or transcription factors (glucocorticoid receptor, 'TFIIH, and E2F). These results show that PML bodies are not prominent sites of RNA synthesis or RNA splicing. We found that a large fraction of PML bodies (50 to 80%) is closely associated with DNA replication domains during exclusively middle-late S-phase. Furthermore, in most cells that we analysed we found at least one PML body was tightly associated with a coiled body. In the APL cell line NB4, the PML gene is fused with the RARa gene due to a chromosomal rearrangement. PML bodies have disappeared and the PML antigen, i.e., PML and the PML-RAR fusion protcin, is dispersed in a punctated pattern throughout the nucleoplasm. We showed that in NB4 cells the sites that are rich in PML antigen significantly colocalize with sites at which nascent RNA accumulates. This suggests that, in contrast to non-APL cells, in NB4 cells the PML antigen is associated with sites of transcription. The implications of these findings for the function of PML bod...

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Localization of Nuclear Functions as Revealed by Ultrastructural Autoradiography and Cytochemistry

Cited by 87 publications

References 188 publications

Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries

Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries

The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.

PML-containing nuclear bodies: Their spatial distribution in relation to other nuclear components

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