Localization of nitric oxide synthase in saphenous vein grafts harvested with a novel “no-touch” technique: Potential role of nitric oxide contribution to improved early graft patency rates

Abstract

The new no-touch technique of saphenous vein harvesting preserves nitric oxide synthase, which suggests that improved nitric oxide availability may be an important mechanism in the success of this technique.

Effects of elevated perfusion pressure and pulsatile flow on human saphenous veins isolated from diabetic and non-diabetic patients

“…11,17,18 The immunohistochemical data With respect to the immunohistochemical expression of NOS isoforms, positive staining associated with eNOS, iNOS and nNOS was observed in all vein layers and in all groups, confirming the findings of previous studies. 7,13,18,19 In the present study, expression of eNOS in the endothelium was not decreased, confirming the findings of Dalio et al 11 Shear stress in the presence of flow is a stimulus for eNOS endothelial expression, resulting in consequential synthesis and release of NO in the remaining endothelium. 14,15 In addition, expression of eNOS was not reduced in the venous walls of HSVs from non-diabetic patients.…”

Coronary vein graft disease: Pathogenesis and prevention

“…A new method of harvesting vein grafts, the so-called 'no-touch' technique, whereby the graft is harvested with the surrounding tissue intact and manual distention is avoided, has been described. Tsui et al (35) examined excess samples from harvested saphenous veins from 10 patients undergoing CABG. The distal portion was stripped of its adventitia and distended, whereas the proximal portion was harvested using the no-touch technique.…”

Buccal mucosa is a promising graft in Peyronie’s disease surgery. Our experience and a brief literature review on autologous grafting materials

“…Prior of these evidences, the venous graft was advocated as the more physiologic than others autologous tissue (30)(31). The advantages presented to support this use were several (32)(33)(34)(35). As reported in Table 3, overall success rates for straightening of the penis with the use of saphenous vein grafting ranged between 66.6% and 96% (36)(37)(38)(39)(40)(41)(42)(43)(44)(45).…”