Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization

Sotelo‐Silveira, José; Calliari, Aldo; Kun, Alejandra; Elizondo, Victoria; Canclini, Lucía; Sotelo, Julio

doi:10.1007/978-1-61779-005-8_8

Cited by 5 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A fragment (nucleotides 3171–6599) of the chicken Myo5a cDNA (clone 32a, kindly provided by Enilza Espreafico) was cloned into pBluescript SK +/−. ISH was carried out according to Sotelo‐Silveira et al () for axonal whole mounts and Sotelo‐Silveira et al () for sciatic nerves. The plasmid was digested at the HPA site and transcribed using the T7 promoter at the 5′ end.…”

Section: Methodsmentioning

confidence: 99%

Myosin Va associates with mRNA in ribonucleoprotein particles present in myelinated peripheral axons and in the central nervous system

Calliari

Farías

Puppo

et al. 2014

Developmental Neurobiology

Self Cite

View full text Add to dashboard Cite

Sorting of specific mRNAs to particular cellular locations and regulation of their translation is an essential mechanism underlying cell polarization. The transport of RNAs by kinesins and dyneins has been clearly established in several cell models, including neurons in culture. A similar role appears to exist in higher eukaryotes for the myosins. Myosin Va (Myo5a) has been described as a component of ribonucleoprotein particles (RNPs) in the adult rat nervous system and associated to ZBP1 and ribosomes in ribosomal periaxoplasmic plaques (PARPs), making it a likely candidate for mediating some aspects of RNA transport in neurons. To test this hypothesis, we have characterized RNPs containing Myo5a in adult brains of rats and mice. Microarray analysis of RNAs co-immunoprecipitated with Myo5a indicates that this motor may associate with a specific subpopulation of neuronal mRNAs. We found mRNAs encoding α-synuclein and several proteins with functions in translation in these RNPs. Immunofluorescence analyses of RNPs showed apparent co-localization of Myo5a with ribosomes, mRNA and RNA-binding proteins in discrete structures present both in axons of neurons in culture and in myelinated fibers of medullary roots. Our data suggest that PARPs include RNPs bearing the mRNA coding for Myo5a and are equipped with kinesin and Myo5a molecular motors. In conclusion, we suggest that Myo5a is involved in mRNA trafficking both in the central and peripheral nervous systems.

show abstract

Section: Methodsmentioning

confidence: 99%

Myosin Va associates with mRNA in ribonucleoprotein particles present in myelinated peripheral axons and in the central nervous system

Calliari

Farías

Puppo

et al. 2014

Developmental Neurobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…There is also evidence for the transport of subsets of mRNA traveling down the axon in association with RNA binding and transport proteins such as staufen and fragile X mental retardation protein (Price et al, 2006 ). In addition, both electronmicroscopy and in situ hybridization have revealed discrete ribosomal domains called periaxoplasmic ribosomal plaques (PARPs), distributed at periodic intervals along myelinated vertebrate peripheral axons (Koenig et al, 2000 ; Sotelo-Silveira et al, 2011 ). The weight of evidence, therefore, implies that the machinery for local translation is present in peripheral sensory fibers, particularly A-fibers.…”

Section: Mtorc1 and Primary Afferentsmentioning

confidence: 99%

Axonal protein synthesis and the regulation of primary afferent function

Obara

Hunt

2013

Developmental Neurobiology

View full text Add to dashboard Cite

Local protein synthesis has been demonstrated in the peripheral processes of sensory primary afferents and is thought to contribute to the maintenance of the neuron, to neuronal plasticity following injury and also to regeneration of the axon after damage to the nerve. The mammalian target of rapamycin (mTOR), a master regulator of protein synthesis, integrates a variety of cues that regulate cellular homeostasis and is thought to play a key role in coordinating the neuronal response to environmental challenges. Evidence suggests that activated mTOR is expressed by peripheral nerve fibers, principally by A-nociceptors that rapidly signal noxious stimulation to the central nervous system, but also by a subset of fibers that respond to cold and itch. Inhibition of mTOR complex 1 (mTORC1) has shown that while the acute response to noxious stimulation is unaffected, more complex aspects of pain processing including the setting up and maintenance of chronic pain states can be disrupted suggesting a route for the generation of new drugs for the control of chronic pain. Given the role of mTORC1 in cellular homeostasis, it seems that systemic changes in the physiological state of the body such as occur during illness are likely to modulate the sensitivity of peripheral sensory afferents through mTORC1 signaling pathways. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 269–278, 2014

show abstract

“…Animals were euthanized by exposure to CO 2 . Sciatic nerves were exposed and excised (Sotelo-Silveira et al, 2011). Segments were cultured in DMEM F12 (GIBCO) and treated with GCA (20 mM).…”

Section: Ex Vivo Treatments Of Sciatic Nervesmentioning

confidence: 99%