Localization of mouse parathyroid hormone-like peptide gene (<i>Pthlh</i>) to distal chromosome 6 using interspecific backcross mice and in situ hybridization

Abstract

The single copy parathyroid hormone-like peptide gene (Pthlh) was mapped to distal mouse chromosome 6 using genetic linkage analysis with a panel of DNA samples from interspecific backcross mice. In all 114 meiotic events examined, the Pthlh locus cosegregated with the locus for the Kirsten ras-2 gene (Kras-2) which was previously localized to distal mouse chromosome 6. In addition, Pthlh was localized to chromosome 6 band F-G and the mouse parathyroid hormone Pth was localized to chromosome 7 band F, by in situ hybridization. These studies confirm the previous localization of Pthlh to mouse chromosome 6 using somatic cell hybrids and show that the Pthlh/PTHLH locus is a part of a conserved linkage group between distal mouse chromosome 6 and the proximal segment of the short arm of human chromosome 12.

Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene.

“…The mouse PTHrP gene has been localized to chromosome 6, region F-G (Seldin et al 1992). A literature search for naturally occurring mutations mapping to this region has not disclosed any known natural mouse mutants phenotypically similar to the PTHrP-deficient strain.…”