Localization of membrane‐bound and soluble antigens using peroxidase‐labeled antibodies and preembedding immunoelectron microscopy

Osamura, R. Yoshiyuki

doi:10.1002/jemt.1060020607

Cited by 5 publications

(1 citation statement)

References 24 publications

(25 reference statements)

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“…After removal of the seminal vesicles, the epithelial side was cut in cubes of 1 mm'. The following procedures were performed based on the protocol described by Osamura and Watanabe (1985).…”

Section: Electron Microscopic Immunohistochemistrymentioning

confidence: 99%

Origin of a sperm motility inhibitor from boar seminal plasma

Iwamoto

Tanaka

Osada

et al. 1993

Molecular Reproduction Devel

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We have investigated the origin of the sperm motility inhibitor (SPMI) from boar seminal plasma. SPMI was measured by its capacity to inhibit the motility of demembranated spermatozoa and by an enzyme-linked immunosorbent assay (ELISA). Among the various reproductive and now reproductive tissues and fluids tested, only the seminal vesicle fluid and seminal plasma contained significant amounts of SPMI biological activity and SPMI antigen. Like other seminal vesicle fluid proteins, SPMI is diluted 6- to 8-fold upon ejaculation. By immunohistochemical detection at the light microscope with antibodies obtained from rabbits immunized with SPMI purified from boar seminal plasma, SPMI was found in the cytosol and/or on the plasma membrane bordering the lumen of the seminal vesicles. At the electron microscope level, SPMI appeared to be present only on the surface of the secretory cells. The data indicate that SPMI originates from a single tissue, the seminal vesicle, and suggest that only the mature form present on the luminal surface of the gland can react with the antibody generated from rabbits immunized with the secreted form of SPMI.

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Section: Electron Microscopic Immunohistochemistrymentioning

confidence: 99%

Origin of a sperm motility inhibitor from boar seminal plasma

Iwamoto

Tanaka

Osada

et al. 1993

Molecular Reproduction Devel

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Expression of the differentiation antigen F7D6 in tumorous tissues of Drosophila

Bedian

Jungklaus

1987

Dev. Genet.

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The 63-kDa antigen recognized by the monoclonal antibody F7D6 is present in all Drosophila embryonic cells and disappears from most tissues as each one reaches its final, differentiated state. Larval tissues lose the antigen around the time of hatching, imaginal tissues lose it during metamorphosis, and germ cells lose it during gametogenesis (Bedian et al: Devel Biol 115:105-118, 1986). The nervous system and spontaneously contracting musculature of the gut and gonads are exceptions and remain antigen positive at all stages. The F7D6 antigen appears to be associated with dividing, undifferentiated cells and electrogenic cells. This prompted us to test tumors for antigen presence. We tested four different recessive mutants that give rise to four different types of tumorous transformation: the embryonic tumor Notch, several larval melanotic tumors, the imaginal disc tumor 1(2)gl, and three alleles of the ovarian tumor otu. In all cases, tumorous tissues in homozygotes contained the F7D6 antigen. The electrophoretic mobility of the antigen appeared to be unaltered in tumorous tissues compared to normal cells, but the antigen is expressed at higher levels. The antigen is found on the cytoplasmic surface of plasma membranes and appears to be a marker of undifferentiated normal and tumorous cells. Similarities and differences between the F7D6 antigen and Drosophila c-src protein are discussed.

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Application of preembedding immunohistochemistry in the study of adenohypophysial plurihormonality

1991

J. Elec. Microsc. Tech.

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Localization of membrane‐bound and soluble antigens using peroxidase‐labeled antibodies and preembedding immunoelectron microscopy

Cited by 5 publications

References 24 publications

Origin of a sperm motility inhibitor from boar seminal plasma

Origin of a sperm motility inhibitor from boar seminal plasma

Expression of the differentiation antigen F7D6 in tumorous tissues of Drosophila

Application of preembedding immunohistochemistry in the study of adenohypophysial plurihormonality

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