Localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase

Mizuno, Noboru; Konishi, Akira; Sato, Manabu

doi:10.1002/cne.901640109

Cited by 226 publications

(83 citation statements)

References 16 publications

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“…In addition, the positions of masseter motoneurons within Mo5 observed in our experiments were similar to those noted in previous studies (Mizuno et al 1975;Weijs 1996;Fay and Norgren 1997), indicating that PRV-BA had not been transported by efferents innervating other muscles. Furthermore, the locations of infected medullary neurons outside of the VN and PH were similar to those reported in a manuscript by Fay and Norgren (Fay and Norgren 1997), who also injected PRV-BA into the masseter muscle.…”

Section: Discussionsupporting

confidence: 91%

Transneuronal tracing of vestibulo-trigeminal pathways innervating the masseter muscle in the rat

et al. 2005

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Previous studies reported that the activity of trigeminal motoneurons innervating masseter muscles is modulated by vestibular inputs. We performed the present study to provide an anatomical substrate for these physiological observations. The transynaptic retrograde tracer pseudorabies virus-Bartha (PRV-BA) was injected into multiple sites of the lower third of the superficial layer of the masseter muscle in rats, a subset of which underwent a sympathectomy prior to virus injections, and the animals were euthanized 24-120 hrs later. Labeled masseteric motoneurons were first found in the ipsilateral trigeminal motor nucleus following a 24-hr post inoculation period; subsequent to 72-hr survival times, the number of infected motoneurons increased, and at ≥ 96-hrs many of these cells showed signs of cytopathic changes. Following 72-hr survival times, a few transynaptically-labeled neurons appeared bilaterally in the medial vestibular nucleus (MVe) and the caudal prepositus hypoglossi (PH) and in the ipsilateral spinal vestibular nucleus (SpVe). At survival times of 96-120 hrs, labeled neurons were consistently observed bilaterally in all vestibular nuclei (VN), although the highest concentration of infected cells was located in the caudal part of the MVe, the SpVe, and the caudal portion of PH. The distribution and density of labeling in the VN and PH were similar in sympathectomized and non-sympathectomized rats. These anatomical data provide the first direct evidence that neurons in the VN and PH project bilaterally to populations of motoneurons innervating the lower third of the superficial layer of the masseter muscle. The MVe, PH, and SpVe appear to play a predominant integrative role in producing vestibulo-trigeminal responses.

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Section: Discussionsupporting

confidence: 91%

Transneuronal tracing of vestibulo-trigeminal pathways innervating the masseter muscle in the rat

et al. 2005

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“…In accordance to Mizuno et al, the masseteric labeled motoneurons were located in the dorsolateral part of the trigeminal motor nucleus (Mizuno et al 1975). No contralateral neuronal labeling was observed.…”

Section: Masseter Hrp Labeled Motoneuronssupporting

confidence: 87%

Thyroid Hormones and Motoneurons Development

Ganji¹

2012

Hypothyroidism - Influences and Treatments

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“…1B shows photomicrographs of a subset of the injection sites. Furthermore, b-CT-labeled motoneurons were observed in the lateral and ventral portions of the ipsilateral Mo5 in every rat; these portions of the nucleus have been demonstrated to contain masseter motoneurons (Mizuno et al 1975;Lynch 1985;Giaconi et al 2006). …”

Section: Resultsmentioning

confidence: 95%

A monosynaptic pathway links the vestibular nuclei and masseter muscle motoneurons in rats

et al. 2007

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Physiological evidence indicates that vestibular signals modulate the activity of motoneurons innervating the masseter muscle. Recently, experiments using transynaptic retrograde transport of pseudorabies virus provided anatomical evidence that many neurons concentrated in the dorsomedial part of the parvicellular division of the medial vestibular nucleus (MVePC) and the caudal prepositus hypoglossi (PH) provide inputs to motoneurons innervating the lower third of the superficial layer of the masseter muscle. However, it was not clear whether this vestibulotrigeminal projection was monosynaptic or polysynaptic. The present study sought to determine whether neurons in the MVePC or PH project directly to motoneurons controlling the masseter muscle in rats. For this purpose, an anterograde tracer (biotinylated dextran amine, BDA) was injected into vestibular nuclei (mainly MVePC) or PH and a retrograde tracer (the β-subunit of cholera toxin, b-CT) was injected into the masseter muscle ipsilateral or contralateral to the BDA injection site. Following injections of BDA into the vestibular nuclei or PH, anterogradely-labeled axon terminals were observed bilaterally in the motor trigeminal nucleus (Mo5), particularly in the ventral, medial, and lateral portions of the nucleus; projections to dorsal Mo5 were sparse. In addition, retrogradely-labeled motoneurons were located in the ventral and lateral portions of the ipsilateral Mo5. Moreover, anterogradely-labeled terminals were observed to make contact with motoneurons in the Mo5 that were retrogradely labeled from b-CT injections into the masseter muscle. This study provides direct evidence that a monosynaptic pathway exists between the MVePC and PH and masseter motoneurons.

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Localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase

Cited by 226 publications

References 16 publications

Transneuronal tracing of vestibulo-trigeminal pathways innervating the masseter muscle in the rat

Transneuronal tracing of vestibulo-trigeminal pathways innervating the masseter muscle in the rat

Thyroid Hormones and Motoneurons Development

A monosynaptic pathway links the vestibular nuclei and masseter muscle motoneurons in rats

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