Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments

Chavrier, Philippe; Parton, Robert G.; Hauri, Hans Peter; Simons, Kai; Zerial, Marino

doi:10.1016/0092-8674(90)90369-p

Cited by 1,111 publications

(855 citation statements)

References 54 publications

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“…Th is indicates that at 1 h postinjection, endocytosed I-switch is resident mainly in the lysosomes. Similarly, using GFP RAB-5 as an early endosomal marker 26 and GFP RAB-7 as a late endosomal / lysosomal marker 27 , we determined that the I-switch is resident predominantly in the early and late endosomes at 5 and 17 min, respectively ( Fig. 4a ; Supple mentary Fig.…”

Section: Resultsmentioning

confidence: 93%

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

et al. 2011

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Structural DNA nanotechnology seeks to build synthetic molecular machinery from DNA. DNA nanomachines are artifi cially designed assemblies that switch between defi ned conformations in response to an external cue. Though it has proved possible to create DNA machines and rudimentary walkers, the function of such autonomous DNA-based molecular devices has not yet been achieved inside living organisms. Here we demonstrate the operation of a pH-triggered DNA nanomachine inside the nematode Caenorhabditis elegans . The nanomachine uses fl uorescence resonance energy transfer to effectively map spatiotemporal pH changes associated with endocytosis in wild type as well as mutant worms, demonstrating autonomous function within the organismal milieu in a variety of genetic backgrounds. From this fi rst demonstration of the independent functionality of a DNA nanomachine in vivo , we observe that rationally designed DNA-based molecular devices retain their in vitro functionality with quantitative precision. This positions DNA nanodevices as exciting and powerful tools to interrogate complex biological phenomena.

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Section: Resultsmentioning

confidence: 93%

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

et al. 2011

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“…Rab7 localizes to late endosomes [35] and controls the traffic of membrane proteins from the early endosome to lysosomes [36][37][38][39][40][41]. The enhanced colocalization of β 2 ARs and EGFP-rab7 in late endosomes in the presence of LY294002 suggests that PI 3-kinase is required for the transport of membrane proteins from late endosomes to lysosomes.…”

Section: Discussionmentioning

confidence: 99%

Inhibitors of phosphoinositide 3-kinase cause defects in the postendocytic sorting of β2-adrenergic receptors

Awwad

Iyer

Rosenfeld

et al. 2007

Experimental Cell Research

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Phosphatidylinositol 3-kinase inhibitors have been shown to affect the endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated β 2 -adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endocytosis, most internalized receptors are sorted to the cell surface, but some proportion is sorted to lysosomes for degradation. It is not known what governs the ratio of receptors that recycle versus receptors that undergo degradation. To determine if phosphatidylinositol 3-kinases regulate β 2 -adrenergic receptor trafficking, HEK293 cells stably expressing these receptors were treated with the phosphatidylinositol 3-kinase inhibitors LY294002 or wortmannin. We then studied agonist-induced receptor endocytosis and postendocytic sorting, including recycling and degradation of the internalized receptors. Both inhibitors amplified the internalization of receptors after exposure to the β-agonist isoproterenol, which was attributable to the sorting of a significant fraction of receptors to an intracellular compartment from which receptor recycling did not occur. The initial rate of β 2 -adrenergic receptor endocytosis and the default rate of receptor recycling were not significantly altered. During prolonged exposure to agonist, LY294002 slowed the degradation rate of β 2 -adrenergic receptors and caused the accumulation of receptors within rab7-positive vesicles. These results suggest that phosphatidylinositol 3-kinase inhibitors (1) cause a misrouting of β 2 -adrenergic receptors into vesicles that are neither able to efficiently recycle to the surface nor sort to lysosomes, and (2) delays the movement of receptors from late endosomes to lysosomes.

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“…36,38,39 The time-course images showed that the majority of viral particles were first located in organelles that were positive for DsRed-Rab5 (Supplementary Intracellular trafficking of the engineered lentivirus K-I Joo and P Wang…”

Section: Tracking Of Viral Transport Through Endosomesmentioning

confidence: 99%

Visualization of targeted transduction by engineered lentiviral vectors

Joo

Wang

2008

Gene Ther

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Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments

Cited by 1,111 publications

References 54 publications

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism

Inhibitors of phosphoinositide 3-kinase cause defects in the postendocytic sorting of β2-adrenergic receptors

Visualization of targeted transduction by engineered lentiviral vectors

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