Localization of Locusta-DP in locust CNS and hemolymph satisfies initial hormonal criteria

Patel, Manju; Chung, J. Sook; Kay, I. T.; Mallet, Anthony I.; Gibbon, Charles R.; Thompson, Kevin; Bacon, Jonathan P.; Coast, Geoffrey M.

doi:10.1016/0196-9781(94)90081-7

Cited by 63 publications

(35 citation statements)

References 38 publications

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“…In particular, immunoreactivity was observed in medial NSCs of the brain, which send projections to the CC, and posterior lateral NSCs in the MTGM, which send projections out to abdominal nerves 1 and 2. In all of the other species studied, including R. prolixus, strong CRF-like immunoreactivity has been observed in a subset of the medial NSCs of the brain (Te Brugge et al, 1999;Cabrero et al, 2002;Iaboni et al, 1998;Patel et al, 1994;Veenstra and Hagedorn, 1991;Emery et al, 1994). As well, the immunoreactivity observed in the posterior lateral NSCs in the abdominal neuromeres of the MTGM of R. prolixus is consistent with the immunoreactivity observed in abdominal ganglia of L. migratoria (Patel et al, 1994;Thompson et al, 1995) and M. sexta (Chen et al, 1994).…”

Section: Distribution Of the Rhopr Dhsupporting

confidence: 67%

“…CRF-like immunoreactivity and expression in posterior lateral NSCs in the MTGM of R. prolixus is consistent with the immunoreactivity and expression pattern found in other insects (Patel et al, 1994;Thompson et al, 1995;Chen et al, 1994;Roller et al, 2008). In B. mori, DH 41 and DH 34 CRF-like expression was observed in dorsolateral cells of the abdominal ganglia (2-7), which are thought to be the lateral NSCs, L3.…”

Section: Distribution Of the Rhopr Dhsupporting

confidence: 65%

“…The Rhopr DH sequence has the greatest amino acid identity (50-55%) to the sequences from D. melanogaster, L. migratoria, D. punctata, Z. nevadensis and P. americana. In common with the C-terminal sequence for Locmi DH, the Rhopr DH ends in QQI-amide. It is interesting that the most successful antisera used to look for CRF-like immunoreactivity in R. prolixus (Te Brugge et al, 1999) was that raised against the C-terminal sequence of Locmi DH, residues 29-46, which also ends in LQQI-amide (coupled at the N-terminus to thyroglobulin using glutaraldehyde) (Patel et al, 1994).…”

Section: Comparison Of Rhopr Dh Peptide Sequence With Other Insect Sementioning

confidence: 99%

“…The first insect CRF-like peptide demonstrated to be a true DH was from Locusta migratoria, Locusta DH (Locmi DH) , where it stimulates Malpighian tubule secretion (Coast et al, 1993), is present in NSCs of the brain and abdominal ganglia (Patel et al, 1994) and is released into the haemolymph after feeding Audsley et al, 1997). In R. prolixus we have shown that the medial NSCs of the brain and the posterior lateral NSCs of the MTGM are CRF-like immunoreactive, as are their associated neurohaemal sites (Te Brugge et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Identification of the elusive peptidergic diuretic hormone in the blood-feeding bugRhodnius prolixus: a CRF-related peptide

Brugge

Paluzzi

Schooley

et al. 2011

Journal of Experimental Biology

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SUMMARYProbing of a host and ingestion of a blood-meal in a fifth instar Rhodnius prolixus results in a cascade of tightly integrated events. The huge blood-meal is pumped into the anterior midgut during feeding, then modified by diuresis and stored until it is digested. While serotonin is known to be a diuretic hormone in R. prolixus, a peptidergic factor(s) was also known to play a role in diuresis. In the present study we employed molecular techniques and mass spectrometry to determine the sequence of a native CRF-like peptide from R. prolixus (Rhopr DH). In addition, we confirmed the distribution and localization of Rhopr DH using in situ hybridization and immunohistochemistry, and demonstrated its potent biological activity on both the anterior midgut and Malpighian tubules.

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Section: Distribution Of the Rhopr Dhsupporting

confidence: 67%

Section: Distribution Of the Rhopr Dhsupporting

confidence: 65%

Section: Comparison Of Rhopr Dh Peptide Sequence With Other Insect Sementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of the elusive peptidergic diuretic hormone in the blood-feeding bugRhodnius prolixus: a CRF-related peptide

Brugge

Paluzzi

Schooley

et al. 2011

Journal of Experimental Biology

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“…Coast et al, 1999). In several species, these factors are expressed throughout the central nervous system (CNS) and gut, often within identified neuroendocrine neurons (Cantera and Nässel, 1992;Chen et al, 1994;Patel et al, 1994;Iaboni et al, 1998;Te Brugge et al, 1999;Veenstra and Hagedorn, 1991;Tamarelle et al, 2000;Wiehart et al, 2002a). In some instances, they are co-expressed in the same cells (Thompson et al, 1995).…”

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confidence: 99%

Drosophila CG8422encodes a functional diuretic hormone receptor

Johnson

Bohn

Taghert

2004

Journal of Experimental Biology

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SUMMARYDiuretic hormone 44 (DH) is a bioactive neuropeptide that mediates osmotic balance in a wide variety of insects through increases in cAMP. It is structurally similar to mammalian corticotrophin releasing factor (CRF)peptides. In the moth Manduca and the cricket Acheta,functional studies have shown that its cognate receptor (DH-R) is related to the mammalian CRF receptor. The Drosophila genome contains two genes(CG8422 and CG12370) orthologous to Manduca and Acheta DH-Rs. Here, we present multiple lines of evidence to support the hypothesis that the orphan CG8422 G-protein-coupled receptor is a functional DH-R. When expressed in mammalian cells, CG8422 conferred selective sensitivity to DH, as indicated by translocation of aβ-arrestin-2-GFP reporter from the cytoplasm to the cell membrane. Consistent with its in vivo activities in other insects, DH activation of CG8422 elicited increases in a cAMP reporter system(CRE-luciferase), with an EC50 of 1.7 nmol l-1. CG8422 activation by DH also led to increases in intracellular calcium but at substantially higher doses (EC50 ∼300 nmol l-1). By microarray analysis, the CG8422 transcript was detectable in Drosophila head mRNA of different genotypes and under different environmental conditions. The identification of a Drosophila receptor for the DH neuropeptide provides a basis for genetic analysis of this critical factor's roles in maintaining physiological homeostasis.

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Multifactorial control of the release of hormones from the locust retrocerebral complex

Vullings

Diederen

Veelaert³

et al. 1999

Microsc. Res. Tech.

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Localization of Locusta-DP in locust CNS and hemolymph satisfies initial hormonal criteria

Cited by 63 publications

References 38 publications

Identification of the elusive peptidergic diuretic hormone in the blood-feeding bugRhodnius prolixus: a CRF-related peptide

Identification of the elusive peptidergic diuretic hormone in the blood-feeding bugRhodnius prolixus: a CRF-related peptide

Drosophila CG8422encodes a functional diuretic hormone receptor

Multifactorial control of the release of hormones from the locust retrocerebral complex

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